Proteoglycan isolation and analysis.
Research output: Contribution to journal › Journal article › Research › peer-review
Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.
|Journal||Current Protocols in Cell Biology|
|Pages (from-to)||Unit 10.7|
|Publication status||Published - 2001|
Keywords: Animals; Cells, Cultured; Extracellular Matrix; Glycosaminoglycans; Humans; Proteoglycans