Proteoglycan isolation and analysis.

Research output: Contribution to journalJournal articleResearchpeer-review

Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.
Original languageEnglish
JournalCurrent Protocols in Cell Biology
VolumeChapter 10
Pages (from-to)Unit 10.7
Publication statusPublished - 2001

Bibliographical note

Keywords: Animals; Cells, Cultured; Extracellular Matrix; Glycosaminoglycans; Humans; Proteoglycans

ID: 5160669