Prenatal Ambient Air Pollution, Placental Mitochondrial DNA Content, and Birth Weight in the INMA (Spain) and ENVIRONAGE (Belgium) Birth Cohorts

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Prenatal Ambient Air Pollution, Placental Mitochondrial DNA Content, and Birth Weight in the INMA (Spain) and ENVIRONAGE (Belgium) Birth Cohorts. / Clemente, Diana B. P.; Casas, Maribel; Vilahur, Nadia; Begiristain, Haizea; Bustamante, Mariona; Carsin, Anne-Elie; Fernández, Mariana F.; Fierens, Frans; Gyselaers, Wilfried; Iñiguez, Carmen; Janssen, Bram G.; Lefebvre, Wouter; Llop, Sabrina; Olea, Nicolás; Pedersen, Marie; Pieters, Nicky; Santa Marina, Loreto; Souto, Anna; Tardón, Adonina; Vanpoucke, Charlotte; Vrijheid, Martine; Sunyer, Jordi; Nawrot, Tim S.

In: Environmental Health Perspectives, Vol. 124, No. 5, 05.2016, p. 659-665.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Clemente, DBP, Casas, M, Vilahur, N, Begiristain, H, Bustamante, M, Carsin, A-E, Fernández, MF, Fierens, F, Gyselaers, W, Iñiguez, C, Janssen, BG, Lefebvre, W, Llop, S, Olea, N, Pedersen, M, Pieters, N, Santa Marina, L, Souto, A, Tardón, A, Vanpoucke, C, Vrijheid, M, Sunyer, J & Nawrot, TS 2016, 'Prenatal Ambient Air Pollution, Placental Mitochondrial DNA Content, and Birth Weight in the INMA (Spain) and ENVIRONAGE (Belgium) Birth Cohorts', Environmental Health Perspectives, vol. 124, no. 5, pp. 659-665. https://doi.org/10.1289/ehp.1408981

APA

Clemente, D. B. P., Casas, M., Vilahur, N., Begiristain, H., Bustamante, M., Carsin, A-E., ... Nawrot, T. S. (2016). Prenatal Ambient Air Pollution, Placental Mitochondrial DNA Content, and Birth Weight in the INMA (Spain) and ENVIRONAGE (Belgium) Birth Cohorts. Environmental Health Perspectives, 124(5), 659-665. https://doi.org/10.1289/ehp.1408981

Vancouver

Clemente DBP, Casas M, Vilahur N, Begiristain H, Bustamante M, Carsin A-E et al. Prenatal Ambient Air Pollution, Placental Mitochondrial DNA Content, and Birth Weight in the INMA (Spain) and ENVIRONAGE (Belgium) Birth Cohorts. Environmental Health Perspectives. 2016 May;124(5):659-665. https://doi.org/10.1289/ehp.1408981

Author

Clemente, Diana B. P. ; Casas, Maribel ; Vilahur, Nadia ; Begiristain, Haizea ; Bustamante, Mariona ; Carsin, Anne-Elie ; Fernández, Mariana F. ; Fierens, Frans ; Gyselaers, Wilfried ; Iñiguez, Carmen ; Janssen, Bram G. ; Lefebvre, Wouter ; Llop, Sabrina ; Olea, Nicolás ; Pedersen, Marie ; Pieters, Nicky ; Santa Marina, Loreto ; Souto, Anna ; Tardón, Adonina ; Vanpoucke, Charlotte ; Vrijheid, Martine ; Sunyer, Jordi ; Nawrot, Tim S. / Prenatal Ambient Air Pollution, Placental Mitochondrial DNA Content, and Birth Weight in the INMA (Spain) and ENVIRONAGE (Belgium) Birth Cohorts. In: Environmental Health Perspectives. 2016 ; Vol. 124, No. 5. pp. 659-665.

Bibtex

@article{e6ad5672d286405d860117e97701fd1c,
title = "Prenatal Ambient Air Pollution, Placental Mitochondrial DNA Content, and Birth Weight in the INMA (Spain) and ENVIRONAGE (Belgium) Birth Cohorts",
abstract = "BACKGROUND: Mitochondria are sensitive to environmental toxicants due to their lack of repair capacity. Changes in mitochondrial DNA (mtDNA) content may represent a biologically relevant intermediate outcome in mechanisms linking air pollution and fetal growth restriction.OBJECTIVE: We investigated whether placental mtDNA content is a possible mediator of the association between prenatal NO2 exposure and birth weight.METHODS: We used data from two independent European cohorts: INMA (n=376; Spain) and ENVIRONAGE (n=550; Belgium). Relative placental mtDNA content was determined as the ratio of two mitochondrial genes (MT-ND1 and MTF3212/R3319) to two control genes (RPLP0 and ACTB). Effect estimates for individual cohorts and the pooled dataset were calculated using multiple linear regression and mixed models. We also performed a mediation analysis.RESULTS: Pooled estimates indicated that a 10µg/m(3) increment in average NO2 exposure during pregnancy was associated with a 4.9{\%} decrease in placental mtDNA content (95{\%} confidence interval (CI): -9.3, -0.3{\%}). and a 48g decrease (95{\%} CI: -87, -9g) in birth weight. However, the association with birth weight was significant for INMA (-66g; 95{\%} CI: -111, -23g) but not for ENVIRONAGE (-20g; 95{\%} CI: -101, 62g). Placental mtDNA content was associated with significantly higher mean birth weight (pooled analysis, IQR increase: 140g; 95{\%} CI: 43, 237g). Mediation analysis estimates, which were derived for the INMA cohort only, suggested that 10{\%} (95{\%} CI: 6.6, 13.0g) of the association between prenatal NO2 and birth weight was mediated by changes in placental mtDNA content.CONCLUSION: Our results suggest that mtDNA content can be one of the potential mediators of the association between prenatal air pollution exposure and birth weight.",
author = "Clemente, {Diana B. P.} and Maribel Casas and Nadia Vilahur and Haizea Begiristain and Mariona Bustamante and Anne-Elie Carsin and Fern{\'a}ndez, {Mariana F.} and Frans Fierens and Wilfried Gyselaers and Carmen I{\~n}iguez and Janssen, {Bram G.} and Wouter Lefebvre and Sabrina Llop and Nicol{\'a}s Olea and Marie Pedersen and Nicky Pieters and {Santa Marina}, Loreto and Anna Souto and Adonina Tard{\'o}n and Charlotte Vanpoucke and Martine Vrijheid and Jordi Sunyer and Nawrot, {Tim S}",
year = "2016",
month = "5",
doi = "10.1289/ehp.1408981",
language = "English",
volume = "124",
pages = "659--665",
journal = "Environmental Health Perspectives",
issn = "0091-6765",
publisher = "National Institute of Environmental Health Sciences",
number = "5",

}

RIS

TY - JOUR

T1 - Prenatal Ambient Air Pollution, Placental Mitochondrial DNA Content, and Birth Weight in the INMA (Spain) and ENVIRONAGE (Belgium) Birth Cohorts

AU - Clemente, Diana B. P.

AU - Casas, Maribel

AU - Vilahur, Nadia

AU - Begiristain, Haizea

AU - Bustamante, Mariona

AU - Carsin, Anne-Elie

AU - Fernández, Mariana F.

AU - Fierens, Frans

AU - Gyselaers, Wilfried

AU - Iñiguez, Carmen

AU - Janssen, Bram G.

AU - Lefebvre, Wouter

AU - Llop, Sabrina

AU - Olea, Nicolás

AU - Pedersen, Marie

AU - Pieters, Nicky

AU - Santa Marina, Loreto

AU - Souto, Anna

AU - Tardón, Adonina

AU - Vanpoucke, Charlotte

AU - Vrijheid, Martine

AU - Sunyer, Jordi

AU - Nawrot, Tim S

PY - 2016/5

Y1 - 2016/5

N2 - BACKGROUND: Mitochondria are sensitive to environmental toxicants due to their lack of repair capacity. Changes in mitochondrial DNA (mtDNA) content may represent a biologically relevant intermediate outcome in mechanisms linking air pollution and fetal growth restriction.OBJECTIVE: We investigated whether placental mtDNA content is a possible mediator of the association between prenatal NO2 exposure and birth weight.METHODS: We used data from two independent European cohorts: INMA (n=376; Spain) and ENVIRONAGE (n=550; Belgium). Relative placental mtDNA content was determined as the ratio of two mitochondrial genes (MT-ND1 and MTF3212/R3319) to two control genes (RPLP0 and ACTB). Effect estimates for individual cohorts and the pooled dataset were calculated using multiple linear regression and mixed models. We also performed a mediation analysis.RESULTS: Pooled estimates indicated that a 10µg/m(3) increment in average NO2 exposure during pregnancy was associated with a 4.9% decrease in placental mtDNA content (95% confidence interval (CI): -9.3, -0.3%). and a 48g decrease (95% CI: -87, -9g) in birth weight. However, the association with birth weight was significant for INMA (-66g; 95% CI: -111, -23g) but not for ENVIRONAGE (-20g; 95% CI: -101, 62g). Placental mtDNA content was associated with significantly higher mean birth weight (pooled analysis, IQR increase: 140g; 95% CI: 43, 237g). Mediation analysis estimates, which were derived for the INMA cohort only, suggested that 10% (95% CI: 6.6, 13.0g) of the association between prenatal NO2 and birth weight was mediated by changes in placental mtDNA content.CONCLUSION: Our results suggest that mtDNA content can be one of the potential mediators of the association between prenatal air pollution exposure and birth weight.

AB - BACKGROUND: Mitochondria are sensitive to environmental toxicants due to their lack of repair capacity. Changes in mitochondrial DNA (mtDNA) content may represent a biologically relevant intermediate outcome in mechanisms linking air pollution and fetal growth restriction.OBJECTIVE: We investigated whether placental mtDNA content is a possible mediator of the association between prenatal NO2 exposure and birth weight.METHODS: We used data from two independent European cohorts: INMA (n=376; Spain) and ENVIRONAGE (n=550; Belgium). Relative placental mtDNA content was determined as the ratio of two mitochondrial genes (MT-ND1 and MTF3212/R3319) to two control genes (RPLP0 and ACTB). Effect estimates for individual cohorts and the pooled dataset were calculated using multiple linear regression and mixed models. We also performed a mediation analysis.RESULTS: Pooled estimates indicated that a 10µg/m(3) increment in average NO2 exposure during pregnancy was associated with a 4.9% decrease in placental mtDNA content (95% confidence interval (CI): -9.3, -0.3%). and a 48g decrease (95% CI: -87, -9g) in birth weight. However, the association with birth weight was significant for INMA (-66g; 95% CI: -111, -23g) but not for ENVIRONAGE (-20g; 95% CI: -101, 62g). Placental mtDNA content was associated with significantly higher mean birth weight (pooled analysis, IQR increase: 140g; 95% CI: 43, 237g). Mediation analysis estimates, which were derived for the INMA cohort only, suggested that 10% (95% CI: 6.6, 13.0g) of the association between prenatal NO2 and birth weight was mediated by changes in placental mtDNA content.CONCLUSION: Our results suggest that mtDNA content can be one of the potential mediators of the association between prenatal air pollution exposure and birth weight.

U2 - 10.1289/ehp.1408981

DO - 10.1289/ehp.1408981

M3 - Journal article

C2 - 26317635

VL - 124

SP - 659

EP - 665

JO - Environmental Health Perspectives

JF - Environmental Health Perspectives

SN - 0091-6765

IS - 5

ER -

ID: 143934198