Population genetics of Phytophthora infestans in Denmark reveals dominantly clonal populations and specific alleles linked to metalaxyl-M resistance
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Population genetics of Phytophthora infestans in Denmark reveals dominantly clonal populations and specific alleles linked to metalaxyl-M resistance. / Montes, Melanie Sarah; Nielsen, B. J.; Schmidt, S. G.; Bødker, L.; Kjøller, Rasmus; Rosendahl, Søren.
In: Plant Pathology, Vol. 65, No. 5, 2016, p. 744-753.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Population genetics of Phytophthora infestans in Denmark reveals dominantly clonal populations and specific alleles linked to metalaxyl-M resistance
AU - Montes, Melanie Sarah
AU - Nielsen, B. J.
AU - Schmidt, S. G.
AU - Bødker, L.
AU - Kjøller, Rasmus
AU - Rosendahl, Søren
PY - 2016
Y1 - 2016
N2 - Control of the potato late blight pathogen Phytophthora infestans relies heavily on chemicals. The fungicide metalaxyl-M (Mefenoxam) has played an important role in controlling the disease, but insensitivity to the fungicide in certain isolates is now of major concern. A genetic basis for resistance to metalaxyl suggests the possibility for linking resistance phenotypes to specific population genetic markers, but in order to do this, the population genetic structure and mode of reproduction in a population must first be well described. The dynamics of metalaxyl-M resistance in the Danish population of P. infestans was characterized over the course of the 2013 growing season, as was the population genetic structure, using simple sequence repeat (SSR) genotypes and single nucleotide polymorphism (SNP)-based mitochondrial haplotyping of over 80 isolates. Both mating types A1 and A2 were present in most fields, but tests for recombination showed that clonal reproduction dominates in Danish populations. Genotype was not linked to haplotype and no differentiation was observed at the haplotype level, but rather between fields. Resistance phenotypes were linked to specific SSR alleles, demonstrating the potential for a more precise SNP-based marker system for predicting resistance to metalaxyl-M.
AB - Control of the potato late blight pathogen Phytophthora infestans relies heavily on chemicals. The fungicide metalaxyl-M (Mefenoxam) has played an important role in controlling the disease, but insensitivity to the fungicide in certain isolates is now of major concern. A genetic basis for resistance to metalaxyl suggests the possibility for linking resistance phenotypes to specific population genetic markers, but in order to do this, the population genetic structure and mode of reproduction in a population must first be well described. The dynamics of metalaxyl-M resistance in the Danish population of P. infestans was characterized over the course of the 2013 growing season, as was the population genetic structure, using simple sequence repeat (SSR) genotypes and single nucleotide polymorphism (SNP)-based mitochondrial haplotyping of over 80 isolates. Both mating types A1 and A2 were present in most fields, but tests for recombination showed that clonal reproduction dominates in Danish populations. Genotype was not linked to haplotype and no differentiation was observed at the haplotype level, but rather between fields. Resistance phenotypes were linked to specific SSR alleles, demonstrating the potential for a more precise SNP-based marker system for predicting resistance to metalaxyl-M.
KW - Phytophthora infestans
KW - Linkage disequilibrium
KW - Metalaxyl resistance
KW - Mitochondrial haplotypes
KW - Potato late blight
KW - Simple sequence repeat
U2 - 10.1111/ppa.12462
DO - 10.1111/ppa.12462
M3 - Journal article
AN - SCOPUS:84951019723
VL - 65
SP - 744
EP - 753
JO - Plant Pathology
JF - Plant Pathology
SN - 0032-0862
IS - 5
ER -
ID: 154218268