pH within pores in plant fiber cell walls assessed by Fluorescence Ratio Imaging

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

pH within pores in plant fiber cell walls assessed by Fluorescence Ratio Imaging. / Hidayat, Budi Juliman; Thygesen, Lisbeth Garbrecht; Johansen, Katja Salomon.

In: Cellulose, Vol. 20, No. 3, 2013, p. 1041-1055.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hidayat, BJ, Thygesen, LG & Johansen, KS 2013, 'pH within pores in plant fiber cell walls assessed by Fluorescence Ratio Imaging', Cellulose, vol. 20, no. 3, pp. 1041-1055. https://doi.org/10.1007/s10570-013-9912-8

APA

Hidayat, B. J., Thygesen, L. G., & Johansen, K. S. (2013). pH within pores in plant fiber cell walls assessed by Fluorescence Ratio Imaging. Cellulose, 20(3), 1041-1055. https://doi.org/10.1007/s10570-013-9912-8

Vancouver

Hidayat BJ, Thygesen LG, Johansen KS. pH within pores in plant fiber cell walls assessed by Fluorescence Ratio Imaging. Cellulose. 2013;20(3):1041-1055. https://doi.org/10.1007/s10570-013-9912-8

Author

Hidayat, Budi Juliman ; Thygesen, Lisbeth Garbrecht ; Johansen, Katja Salomon. / pH within pores in plant fiber cell walls assessed by Fluorescence Ratio Imaging. In: Cellulose. 2013 ; Vol. 20, No. 3. pp. 1041-1055.

Bibtex

@article{a9b81b2350cd4228978f37f3e5d1addb,
title = "pH within pores in plant fiber cell walls assessed by Fluorescence Ratio Imaging",
abstract = "The pH within cell wall pores of filter paper fibers and hemp fibers was assessed by Fluorescence Ratio Imaging (FRIM). It was found that the Donnan effect affected the pH measured within the fibers. When the conductivity of the added liquid was low (0. 7 mS), pH values were lower within the cell wall than in the bulk solution. This was not the case at high conductivity (22 mS). The occurrence of the Donnan effect allowed the pH values within pores in normal regions of the cell wall to be compared to the pH in regions with misaligned microfibrils (dislocations) when FRIM was carried out in a low conductivity solution. Surprisingly, no pH difference was observed between normal regions and dislocations, suggesting that pore sizes within the two different regions are approximately the same. In another experiment the Donnan effect was shown to have an effect on hydrolysis of hydrothermally pretreated wheat straw only when conducted in a low conductivity solution and only for xylanase, not cellulases. The hydrolysis experiments indicate that under typical conditions where conductivity is high, the Donnan effect does not lower the pH close to the substrate to an extent that affects enzymatic activity during hydrolysis of lignocellulose.",
author = "Hidayat, {Budi Juliman} and Thygesen, {Lisbeth Garbrecht} and Johansen, {Katja Salomon}",
year = "2013",
doi = "10.1007/s10570-013-9912-8",
language = "English",
volume = "20",
pages = "1041--1055",
journal = "Cellulose",
issn = "0969-0239",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - pH within pores in plant fiber cell walls assessed by Fluorescence Ratio Imaging

AU - Hidayat, Budi Juliman

AU - Thygesen, Lisbeth Garbrecht

AU - Johansen, Katja Salomon

PY - 2013

Y1 - 2013

N2 - The pH within cell wall pores of filter paper fibers and hemp fibers was assessed by Fluorescence Ratio Imaging (FRIM). It was found that the Donnan effect affected the pH measured within the fibers. When the conductivity of the added liquid was low (0. 7 mS), pH values were lower within the cell wall than in the bulk solution. This was not the case at high conductivity (22 mS). The occurrence of the Donnan effect allowed the pH values within pores in normal regions of the cell wall to be compared to the pH in regions with misaligned microfibrils (dislocations) when FRIM was carried out in a low conductivity solution. Surprisingly, no pH difference was observed between normal regions and dislocations, suggesting that pore sizes within the two different regions are approximately the same. In another experiment the Donnan effect was shown to have an effect on hydrolysis of hydrothermally pretreated wheat straw only when conducted in a low conductivity solution and only for xylanase, not cellulases. The hydrolysis experiments indicate that under typical conditions where conductivity is high, the Donnan effect does not lower the pH close to the substrate to an extent that affects enzymatic activity during hydrolysis of lignocellulose.

AB - The pH within cell wall pores of filter paper fibers and hemp fibers was assessed by Fluorescence Ratio Imaging (FRIM). It was found that the Donnan effect affected the pH measured within the fibers. When the conductivity of the added liquid was low (0. 7 mS), pH values were lower within the cell wall than in the bulk solution. This was not the case at high conductivity (22 mS). The occurrence of the Donnan effect allowed the pH values within pores in normal regions of the cell wall to be compared to the pH in regions with misaligned microfibrils (dislocations) when FRIM was carried out in a low conductivity solution. Surprisingly, no pH difference was observed between normal regions and dislocations, suggesting that pore sizes within the two different regions are approximately the same. In another experiment the Donnan effect was shown to have an effect on hydrolysis of hydrothermally pretreated wheat straw only when conducted in a low conductivity solution and only for xylanase, not cellulases. The hydrolysis experiments indicate that under typical conditions where conductivity is high, the Donnan effect does not lower the pH close to the substrate to an extent that affects enzymatic activity during hydrolysis of lignocellulose.

UR - http://www.scopus.com/inward/record.url?scp=84877628509&partnerID=8YFLogxK

U2 - 10.1007/s10570-013-9912-8

DO - 10.1007/s10570-013-9912-8

M3 - Journal article

AN - SCOPUS:84877628509

VL - 20

SP - 1041

EP - 1055

JO - Cellulose

JF - Cellulose

SN - 0969-0239

IS - 3

ER -

ID: 97315570