p38-MK2 signaling axis regulates RNA metabolism after UV-light-induced DNA damage

Research output: Contribution to journalJournal articlepeer-review

Standard

p38-MK2 signaling axis regulates RNA metabolism after UV-light-induced DNA damage. / Borisova, Marina E; Voigt, Andrea; Tollenaere, Maxim A.X.; Sahu, Sanjeeb Kumar; Juretschke, Thomas; Kreim, Nastasja; Mailand, Niels; Choudhary, Chunaram; Bekker-Jensen, Simon; Akutsu, Masato; Wagner, Sebastian A; Beli, Petra.

In: Nature Communications, Vol. 9, 1017, 01.12.2018, p. 1-16.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Borisova, ME, Voigt, A, Tollenaere, MAX, Sahu, SK, Juretschke, T, Kreim, N, Mailand, N, Choudhary, C, Bekker-Jensen, S, Akutsu, M, Wagner, SA & Beli, P 2018, 'p38-MK2 signaling axis regulates RNA metabolism after UV-light-induced DNA damage', Nature Communications, vol. 9, 1017, pp. 1-16. https://doi.org/10.1038/s41467-018-03417-3

APA

Borisova, M. E., Voigt, A., Tollenaere, M. A. X., Sahu, S. K., Juretschke, T., Kreim, N., Mailand, N., Choudhary, C., Bekker-Jensen, S., Akutsu, M., Wagner, S. A., & Beli, P. (2018). p38-MK2 signaling axis regulates RNA metabolism after UV-light-induced DNA damage. Nature Communications, 9, 1-16. [1017]. https://doi.org/10.1038/s41467-018-03417-3

Vancouver

Borisova ME, Voigt A, Tollenaere MAX, Sahu SK, Juretschke T, Kreim N et al. p38-MK2 signaling axis regulates RNA metabolism after UV-light-induced DNA damage. Nature Communications. 2018 Dec 1;9:1-16. 1017. https://doi.org/10.1038/s41467-018-03417-3

Author

Borisova, Marina E ; Voigt, Andrea ; Tollenaere, Maxim A.X. ; Sahu, Sanjeeb Kumar ; Juretschke, Thomas ; Kreim, Nastasja ; Mailand, Niels ; Choudhary, Chunaram ; Bekker-Jensen, Simon ; Akutsu, Masato ; Wagner, Sebastian A ; Beli, Petra. / p38-MK2 signaling axis regulates RNA metabolism after UV-light-induced DNA damage. In: Nature Communications. 2018 ; Vol. 9. pp. 1-16.

Bibtex

@article{366778a2b1734190b400b20355632b6c,
title = "p38-MK2 signaling axis regulates RNA metabolism after UV-light-induced DNA damage",
abstract = "Ultraviolet (UV) light radiation induces the formation of bulky photoproducts in the DNA that globally affect transcription and splicing. However, the signaling pathways and mechanisms that link UV-light-induced DNA damage to changes in RNA metabolism remain poorly understood. Here we employ quantitative phosphoproteomics and protein kinase inhibition to provide a systems view on protein phosphorylation patterns induced by UV light and uncover the dependencies of phosphorylation events on the canonical DNA damage signaling by ATM/ATR and the p38 MAP kinase pathway. We identify RNA-binding proteins as primary substrates and 14-3-3 as direct readers of p38-MK2-dependent phosphorylation induced by UV light. Mechanistically, we show that MK2 phosphorylates the RNA-binding subunit of the NELF complex NELFE on Serine 115. NELFE phosphorylation promotes the recruitment of 14-3-3 and rapid dissociation of the NELF complex from chromatin, which is accompanied by RNA polymerase II elongation.",
author = "Borisova, {Marina E} and Andrea Voigt and Tollenaere, {Maxim A.X.} and Sahu, {Sanjeeb Kumar} and Thomas Juretschke and Nastasja Kreim and Niels Mailand and Chunaram Choudhary and Simon Bekker-Jensen and Masato Akutsu and Wagner, {Sebastian A} and Petra Beli",
year = "2018",
month = dec,
day = "1",
doi = "10.1038/s41467-018-03417-3",
language = "English",
volume = "9",
pages = "1--16",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - p38-MK2 signaling axis regulates RNA metabolism after UV-light-induced DNA damage

AU - Borisova, Marina E

AU - Voigt, Andrea

AU - Tollenaere, Maxim A.X.

AU - Sahu, Sanjeeb Kumar

AU - Juretschke, Thomas

AU - Kreim, Nastasja

AU - Mailand, Niels

AU - Choudhary, Chunaram

AU - Bekker-Jensen, Simon

AU - Akutsu, Masato

AU - Wagner, Sebastian A

AU - Beli, Petra

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Ultraviolet (UV) light radiation induces the formation of bulky photoproducts in the DNA that globally affect transcription and splicing. However, the signaling pathways and mechanisms that link UV-light-induced DNA damage to changes in RNA metabolism remain poorly understood. Here we employ quantitative phosphoproteomics and protein kinase inhibition to provide a systems view on protein phosphorylation patterns induced by UV light and uncover the dependencies of phosphorylation events on the canonical DNA damage signaling by ATM/ATR and the p38 MAP kinase pathway. We identify RNA-binding proteins as primary substrates and 14-3-3 as direct readers of p38-MK2-dependent phosphorylation induced by UV light. Mechanistically, we show that MK2 phosphorylates the RNA-binding subunit of the NELF complex NELFE on Serine 115. NELFE phosphorylation promotes the recruitment of 14-3-3 and rapid dissociation of the NELF complex from chromatin, which is accompanied by RNA polymerase II elongation.

AB - Ultraviolet (UV) light radiation induces the formation of bulky photoproducts in the DNA that globally affect transcription and splicing. However, the signaling pathways and mechanisms that link UV-light-induced DNA damage to changes in RNA metabolism remain poorly understood. Here we employ quantitative phosphoproteomics and protein kinase inhibition to provide a systems view on protein phosphorylation patterns induced by UV light and uncover the dependencies of phosphorylation events on the canonical DNA damage signaling by ATM/ATR and the p38 MAP kinase pathway. We identify RNA-binding proteins as primary substrates and 14-3-3 as direct readers of p38-MK2-dependent phosphorylation induced by UV light. Mechanistically, we show that MK2 phosphorylates the RNA-binding subunit of the NELF complex NELFE on Serine 115. NELFE phosphorylation promotes the recruitment of 14-3-3 and rapid dissociation of the NELF complex from chromatin, which is accompanied by RNA polymerase II elongation.

U2 - 10.1038/s41467-018-03417-3

DO - 10.1038/s41467-018-03417-3

M3 - Journal article

C2 - 29523821

VL - 9

SP - 1

EP - 16

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 1017

ER -

ID: 192400297