Obesity shows preserved plasma proteome in large independent clinical cohorts
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Obesity shows preserved plasma proteome in large independent clinical cohorts. / Cominetti, Ornella; Núñez Galindo, Antonio; Corthésy, John; Valsesia, Armand; Irincheeva, Irina; Kussmann, Martin; Saris, Wim H M; Astrup, Arne; McPherson, Ruth; Harper, Mary-Ellen; Dent, Robert; Hager, Jörg; Dayon, Loïc.
In: Scientific Reports, Vol. 8, 16981, 2018.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Obesity shows preserved plasma proteome in large independent clinical cohorts
AU - Cominetti, Ornella
AU - Núñez Galindo, Antonio
AU - Corthésy, John
AU - Valsesia, Armand
AU - Irincheeva, Irina
AU - Kussmann, Martin
AU - Saris, Wim H M
AU - Astrup, Arne
AU - McPherson, Ruth
AU - Harper, Mary-Ellen
AU - Dent, Robert
AU - Hager, Jörg
AU - Dayon, Loïc
N1 - CURIS 2018 NEXS 389
PY - 2018
Y1 - 2018
N2 - Holistic human proteome maps are expected to complement comprehensive profile assessment of health and disease phenotypes. However, methodologies to analyze proteomes in human tissue or body fluid samples at relevant scale and performance are still limited in clinical research. Their deployment and demonstration in large enough human populations are even sparser. In the present study, we have characterized and compared the plasma proteomes of two large independent cohorts of obese and overweight individuals using shotgun mass spectrometry (MS)-based proteomics. Herein, we showed, in both populations from different continents of about 500 individuals each, the concordance of plasma protein MS measurements in terms of variability, gender-specificity, and age-relationship. Additionally, we replicated several known and new associations between proteins, clinical and molecular variables, such as insulin and glucose concentrations. In conclusion, our MS-based analyses of plasma samples from independent human cohorts proved the practical feasibility and efficiency of a large and unified discovery/replication approach in proteomics, which was also recently coined “rectangular” design.
AB - Holistic human proteome maps are expected to complement comprehensive profile assessment of health and disease phenotypes. However, methodologies to analyze proteomes in human tissue or body fluid samples at relevant scale and performance are still limited in clinical research. Their deployment and demonstration in large enough human populations are even sparser. In the present study, we have characterized and compared the plasma proteomes of two large independent cohorts of obese and overweight individuals using shotgun mass spectrometry (MS)-based proteomics. Herein, we showed, in both populations from different continents of about 500 individuals each, the concordance of plasma protein MS measurements in terms of variability, gender-specificity, and age-relationship. Additionally, we replicated several known and new associations between proteins, clinical and molecular variables, such as insulin and glucose concentrations. In conclusion, our MS-based analyses of plasma samples from independent human cohorts proved the practical feasibility and efficiency of a large and unified discovery/replication approach in proteomics, which was also recently coined “rectangular” design.
U2 - 10.1038/s41598-018-35321-7
DO - 10.1038/s41598-018-35321-7
M3 - Journal article
C2 - 30451909
VL - 8
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 16981
ER -
ID: 209089422