NetworKIN: a resource for exploring cellular phosphorylation networks

Research output: Contribution to journalJournal articlepeer-review

  • Rune Linding
  • Jensen, Lars Juhl
  • Adrian Pasculescu
  • Marina Olhovsky
  • Karen Colwill
  • Peer Bork
  • Michael B Yaffe
  • Tony Pawson
Protein kinases control cellular responses by phosphorylating specific substrates. Recent proteome-wide mapping of protein phosphorylation sites by mass spectrometry has discovered thousands of in vivo sites. Systematically assigning all 518 human kinases to all these sites is a challenging problem. The NetworKIN database ( integrates consensus substrate motifs with context modelling for improved prediction of cellular kinase-substrate relations. Based on the latest human phosphoproteome from the Phospho.ELM and PhosphoSite databases, the resource offers insight into phosphorylation-modulated interaction networks. Here, we describe how NetworKIN can be used for both global and targeted molecular studies. Via the web interface users can query the database of precomputed kinase-substrate relations or obtain predictions on novel phosphoproteins. The database currently contains a predicted phosphorylation network with 20,224 site-specific interactions involving 3978 phosphoproteins and 73 human kinases from 20 families.
Original languageEnglish
JournalNucleic Acids Research
Issue numberDatabase issue
Pages (from-to)D695-9
Publication statusPublished - 2008
Externally publishedYes

ID: 40740202