TY - JOUR

T1 - Multi-species colloidosomes by surface-modified lactic acid bacteria with enhanced aggregation properties

AU - Jiang, Xiaoyi

AU - Martens, Helle Jakobe

AU - Shekarforoush, Elhamalsadat

AU - Muhammed, Musemma Kedir

AU - Whitehead, Kathryn A.

AU - Arneborg, Nils

AU - Risbo, Jens

N1 - Publisher Copyright: © 2022

PY - 2022

Y1 - 2022

N2 - Hypothesis: Surface modification of lactic acid bacteria enhances their adsorption and aggregation at air–water interface and enables stabilization of microbubbles that spontaneously transform into water-filled colloidosomes, which can be further modified using LBL formulations. Experiments: The bacterial physicochemical properties were characterized using water contact angle (WCA) measurement, bacterial aggregation assay and zeta potential measurement. Cell viability was enumerated using plate-counting method. The LBL reinforcement of colloidosomes was examined by zeta potential measurement and the formed microstructure was investigated using bright-field microscopy, confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Shell permeability of colloidosomes was evaluated using a dye release study. Findings: Bacteria surface-modified using octenyl succinic anhydride (OSA) expressed strong adsorption and aggregation at air–water interface when producing microbubbles. Bacteria with enhanced aggregation ability formed stable shells, enabling complete removal of air and air–water interface without shell disintegration. The formed colloidosomes were studied as they were, or were further reinforced by LBL deposition using polymer or hybrid formulations. Hybrid coating involved assembly of two bacterial species producing colloidosomes with low shell porosity. The findings can be exploited to organize different living bacteria into structured materials and to encapsulate and release substances of diverse sizes and surface properties.

AB - Hypothesis: Surface modification of lactic acid bacteria enhances their adsorption and aggregation at air–water interface and enables stabilization of microbubbles that spontaneously transform into water-filled colloidosomes, which can be further modified using LBL formulations. Experiments: The bacterial physicochemical properties were characterized using water contact angle (WCA) measurement, bacterial aggregation assay and zeta potential measurement. Cell viability was enumerated using plate-counting method. The LBL reinforcement of colloidosomes was examined by zeta potential measurement and the formed microstructure was investigated using bright-field microscopy, confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Shell permeability of colloidosomes was evaluated using a dye release study. Findings: Bacteria surface-modified using octenyl succinic anhydride (OSA) expressed strong adsorption and aggregation at air–water interface when producing microbubbles. Bacteria with enhanced aggregation ability formed stable shells, enabling complete removal of air and air–water interface without shell disintegration. The formed colloidosomes were studied as they were, or were further reinforced by LBL deposition using polymer or hybrid formulations. Hybrid coating involved assembly of two bacterial species producing colloidosomes with low shell porosity. The findings can be exploited to organize different living bacteria into structured materials and to encapsulate and release substances of diverse sizes and surface properties.

KW - Adsorption

KW - Aggregation

KW - Colloidosome

KW - Lactic acid bacteria

KW - Layer-by-layer

KW - Microbubbles

KW - Octenyl succinic anhydride

U2 - 10.1016/j.jcis.2022.04.136

DO - 10.1016/j.jcis.2022.04.136

M3 - Journal article

C2 - 35526410

AN - SCOPUS:85129538963

VL - 622

SP - 503

EP - 514

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

ER -