Mucoadhesive Electrospun Nanofiber-Based Hybrid System with Controlled and Unidirectional Release of Desmopressin
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The sublingual mucosa is an attractive route for drug delivery, although challenged by a continuous flow of saliva that leads to a loss of drug by swallowing. It is of great benefit that drugs absorbed across the sublingual mucosa avoid exposure to the harsh environment of the gastro-in-testinal lumen; this is especially beneficial for drugs of low physicochemical stability such as therapeutic peptides. In this study, a two-layered hybrid drug delivery system was developed for the sublingual delivery of the therapeutic peptide desmopressin. It consisted of peptide-loaded muco-adhesive electrospun chitosan/polyethylene oxide-based nanofibers (mean diameter of 183 ± 20 nm) and a saliva-repelling backing film to promote unidirectional release towards the mucosa. Desmo-pressin was released from the nanofiber-based hybrid system (approximately 80% of the loaded peptide was released within 45 min) in a unidirectional manner in vitro. Importantly, the nanofiber– film hybrid system protected the peptide from wash-out, as demonstrated in an ex vivo flow retention model with porcine sublingual mucosal tissue. Approximately 90% of the loaded desmopressin was retained at the surface of the ex vivo porcine sublingual mucosa after 15 min of exposure to flow rates representing salivary flow.
|Journal||International Journal of Molecular Sciences|
|Number of pages||9|
|Publication status||Published - 2022|
Funding: This research was funded by The Danish Council for Independent Research, Technology and Production, grant number DFF-6111-00333; the Novo Nordisk Foundation Grand Challenge Program, NNF16OC0021948; and Innovation Fund Denmark PROBIO, project-7076-00053B.
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
- Biopharmaceuticals, Electrospinning, Ex vivo flow retention model, Muco-adhesion, Peptide drug delivery, Sublingual delivery
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