Monitoring plasmid-mediated horizontal gene transfer in microbiomes

Monitoring plasmid-mediated horizontal gene transfer in microbiomes: recent advances and future perspectives

Research output: Contribution to journal › Review › Research › peer-review

Standard

Monitoring plasmid-mediated horizontal gene transfer in microbiomes : recent advances and future perspectives. / Pinilla Redondo, Rafael; Cyriaque, Valentine; Jacquiod, Samuel; Sørensen, Søren Johannes; Riber, Leise.

In: Plasmid, Vol. 99, 2018, p. 56-67.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Pinilla Redondo, R, Cyriaque, V, Jacquiod, S, Sørensen, SJ & Riber, L 2018, 'Monitoring plasmid-mediated horizontal gene transfer in microbiomes: recent advances and future perspectives', Plasmid, vol. 99, pp. 56-67. https://doi.org/10.1016/j.plasmid.2018.08.002

APA

Pinilla Redondo, R., Cyriaque, V., Jacquiod, S., Sørensen, S. J., & Riber, L. (2018). Monitoring plasmid-mediated horizontal gene transfer in microbiomes: recent advances and future perspectives. Plasmid, 99, 56-67. https://doi.org/10.1016/j.plasmid.2018.08.002

Vancouver

Pinilla Redondo R, Cyriaque V, Jacquiod S, Sørensen SJ, Riber L. Monitoring plasmid-mediated horizontal gene transfer in microbiomes: recent advances and future perspectives. Plasmid. 2018;99:56-67. https://doi.org/10.1016/j.plasmid.2018.08.002

Author

Pinilla Redondo, Rafael ; Cyriaque, Valentine ; Jacquiod, Samuel ; Sørensen, Søren Johannes ; Riber, Leise. / Monitoring plasmid-mediated horizontal gene transfer in microbiomes : recent advances and future perspectives. In: Plasmid. 2018 ; Vol. 99. pp. 56-67.

Bibtex

@article{ae7401cf512a4a22ae8de426ab26d573,

title = "Monitoring plasmid-mediated horizontal gene transfer in microbiomes: recent advances and future perspectives",

abstract = "The emergence of antimicrobial resistant bacteria constitutes an increasing global health concern. Although it is well recognized that the cornerstone underlying this phenomenon is the dissemination of antimicrobial resistance via plasmids and other mobile genetic elements, the antimicrobial resistance transfer routes remain largely uncharted. In this review, we describe different methods for assessing the transfer frequency and host ranges of plasmids within complex microbiomes. The discussion is centered around the critical evaluation of recent advances for monitoring the fate of fluorescently tagged plasmids in bacterial communities through the coupling of fluorescence activated cell sorting and next generation sequencing techniques. We argue that this approach constitutes an exceptional tool for obtaining quantitative data regarding the extent of plasmid transfer, key disseminating taxa, and possible propagation routes. The integration of this information will provide valuable insights on how to develop alternative avenues for fighting the rise of antimicrobial resistant pathogens, as well as the means for constructing more comprehensive risk assessment models.",

author = "{Pinilla Redondo}, Rafael and Valentine Cyriaque and Samuel Jacquiod and S{\o}rensen, {S{\o}ren Johannes} and Leise Riber",

year = "2018",

doi = "10.1016/j.plasmid.2018.08.002",

language = "English",

volume = "99",

pages = "56--67",

journal = "Plasmid",

issn = "0147-619X",

publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Monitoring plasmid-mediated horizontal gene transfer in microbiomes

T2 - recent advances and future perspectives

AU - Pinilla Redondo, Rafael

AU - Cyriaque, Valentine

AU - Jacquiod, Samuel

AU - Sørensen, Søren Johannes

AU - Riber, Leise

PY - 2018

Y1 - 2018

N2 - The emergence of antimicrobial resistant bacteria constitutes an increasing global health concern. Although it is well recognized that the cornerstone underlying this phenomenon is the dissemination of antimicrobial resistance via plasmids and other mobile genetic elements, the antimicrobial resistance transfer routes remain largely uncharted. In this review, we describe different methods for assessing the transfer frequency and host ranges of plasmids within complex microbiomes. The discussion is centered around the critical evaluation of recent advances for monitoring the fate of fluorescently tagged plasmids in bacterial communities through the coupling of fluorescence activated cell sorting and next generation sequencing techniques. We argue that this approach constitutes an exceptional tool for obtaining quantitative data regarding the extent of plasmid transfer, key disseminating taxa, and possible propagation routes. The integration of this information will provide valuable insights on how to develop alternative avenues for fighting the rise of antimicrobial resistant pathogens, as well as the means for constructing more comprehensive risk assessment models.

AB - The emergence of antimicrobial resistant bacteria constitutes an increasing global health concern. Although it is well recognized that the cornerstone underlying this phenomenon is the dissemination of antimicrobial resistance via plasmids and other mobile genetic elements, the antimicrobial resistance transfer routes remain largely uncharted. In this review, we describe different methods for assessing the transfer frequency and host ranges of plasmids within complex microbiomes. The discussion is centered around the critical evaluation of recent advances for monitoring the fate of fluorescently tagged plasmids in bacterial communities through the coupling of fluorescence activated cell sorting and next generation sequencing techniques. We argue that this approach constitutes an exceptional tool for obtaining quantitative data regarding the extent of plasmid transfer, key disseminating taxa, and possible propagation routes. The integration of this information will provide valuable insights on how to develop alternative avenues for fighting the rise of antimicrobial resistant pathogens, as well as the means for constructing more comprehensive risk assessment models.

U2 - 10.1016/j.plasmid.2018.08.002

DO - 10.1016/j.plasmid.2018.08.002

M3 - Review

C2 - 30086339

VL - 99

SP - 56

EP - 67

JO - Plasmid

JF - Plasmid

SN - 0147-619X

ER -

ID: 200858797