Mitochondria as determinant of nucleotide pools and chromosomal stability

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Mitochondria as determinant of nucleotide pools and chromosomal stability. / Desler, Claus; Munch-Petersen, Birgitte; Stevnsner, Tinna; Matsui, Sei-Ichi; Kulawiec, Mariola; Singh, Keshav K; Rasmussen, Lene Juel.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 625, No. 1-2, 01.12.2007, p. 112-24.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Desler, C, Munch-Petersen, B, Stevnsner, T, Matsui, S-I, Kulawiec, M, Singh, KK & Rasmussen, LJ 2007, 'Mitochondria as determinant of nucleotide pools and chromosomal stability', Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, vol. 625, no. 1-2, pp. 112-24. https://doi.org/10.1016/j.mrfmmm.2007.06.002

APA

Desler, C., Munch-Petersen, B., Stevnsner, T., Matsui, S-I., Kulawiec, M., Singh, K. K., & Rasmussen, L. J. (2007). Mitochondria as determinant of nucleotide pools and chromosomal stability. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 625(1-2), 112-24. https://doi.org/10.1016/j.mrfmmm.2007.06.002

Vancouver

Desler C, Munch-Petersen B, Stevnsner T, Matsui S-I, Kulawiec M, Singh KK et al. Mitochondria as determinant of nucleotide pools and chromosomal stability. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2007 Dec 1;625(1-2):112-24. https://doi.org/10.1016/j.mrfmmm.2007.06.002

Author

Desler, Claus ; Munch-Petersen, Birgitte ; Stevnsner, Tinna ; Matsui, Sei-Ichi ; Kulawiec, Mariola ; Singh, Keshav K ; Rasmussen, Lene Juel. / Mitochondria as determinant of nucleotide pools and chromosomal stability. In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2007 ; Vol. 625, No. 1-2. pp. 112-24.

Bibtex

@article{de972472d1c2451fb3b980e0151cef99,
title = "Mitochondria as determinant of nucleotide pools and chromosomal stability",
abstract = "Mitochondrial function plays an important role in multiple human diseases and mutations in the mitochondrial genome have been detected in nearly every type of cancer investigated to date. However, the mechanism underlying the interrelation is unknown. We used human cell lines depleted of mitochondrial DNA as models and analyzed the outcome of mitochondrial dysfunction on major cellular repair activities. We show that the deoxyribonucleoside triphosphate (dNTP) pools are affected, most prominently we detect a 3-fold reduction of the dTTP pool when normalized to the number of cells in S-phase. It is known that imbalanced dNTP pools are mutagenic and in accordance, we show that mitochondrial dysfunction results in chromosomal instability, which can explain its role in tumor development. We did not find any straightforward correlation between ATP levels and dNTP pools in cells with defective mitochondrial activity. Our results suggest that mitochondria are central players in maintaining genomic stability and in controlling essential nuclear processes such as upholding a balanced supply of nucleotides.",
keywords = "Chromosomal Instability, Comet Assay, DNA Repair, DNA, Mitochondrial, Deoxyribonucleotides, HeLa Cells, Humans, Micronucleus Tests, Mitochondria, Thymine Nucleotides",
author = "Claus Desler and Birgitte Munch-Petersen and Tinna Stevnsner and Sei-Ichi Matsui and Mariola Kulawiec and Singh, {Keshav K} and Rasmussen, {Lene Juel}",
year = "2007",
month = dec,
day = "1",
doi = "10.1016/j.mrfmmm.2007.06.002",
language = "English",
volume = "625",
pages = "112--24",
journal = "Mutation Research Letters",
issn = "0027-5107",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Mitochondria as determinant of nucleotide pools and chromosomal stability

AU - Desler, Claus

AU - Munch-Petersen, Birgitte

AU - Stevnsner, Tinna

AU - Matsui, Sei-Ichi

AU - Kulawiec, Mariola

AU - Singh, Keshav K

AU - Rasmussen, Lene Juel

PY - 2007/12/1

Y1 - 2007/12/1

N2 - Mitochondrial function plays an important role in multiple human diseases and mutations in the mitochondrial genome have been detected in nearly every type of cancer investigated to date. However, the mechanism underlying the interrelation is unknown. We used human cell lines depleted of mitochondrial DNA as models and analyzed the outcome of mitochondrial dysfunction on major cellular repair activities. We show that the deoxyribonucleoside triphosphate (dNTP) pools are affected, most prominently we detect a 3-fold reduction of the dTTP pool when normalized to the number of cells in S-phase. It is known that imbalanced dNTP pools are mutagenic and in accordance, we show that mitochondrial dysfunction results in chromosomal instability, which can explain its role in tumor development. We did not find any straightforward correlation between ATP levels and dNTP pools in cells with defective mitochondrial activity. Our results suggest that mitochondria are central players in maintaining genomic stability and in controlling essential nuclear processes such as upholding a balanced supply of nucleotides.

AB - Mitochondrial function plays an important role in multiple human diseases and mutations in the mitochondrial genome have been detected in nearly every type of cancer investigated to date. However, the mechanism underlying the interrelation is unknown. We used human cell lines depleted of mitochondrial DNA as models and analyzed the outcome of mitochondrial dysfunction on major cellular repair activities. We show that the deoxyribonucleoside triphosphate (dNTP) pools are affected, most prominently we detect a 3-fold reduction of the dTTP pool when normalized to the number of cells in S-phase. It is known that imbalanced dNTP pools are mutagenic and in accordance, we show that mitochondrial dysfunction results in chromosomal instability, which can explain its role in tumor development. We did not find any straightforward correlation between ATP levels and dNTP pools in cells with defective mitochondrial activity. Our results suggest that mitochondria are central players in maintaining genomic stability and in controlling essential nuclear processes such as upholding a balanced supply of nucleotides.

KW - Chromosomal Instability

KW - Comet Assay

KW - DNA Repair

KW - DNA, Mitochondrial

KW - Deoxyribonucleotides

KW - HeLa Cells

KW - Humans

KW - Micronucleus Tests

KW - Mitochondria

KW - Thymine Nucleotides

U2 - 10.1016/j.mrfmmm.2007.06.002

DO - 10.1016/j.mrfmmm.2007.06.002

M3 - Journal article

C2 - 17658559

VL - 625

SP - 112

EP - 124

JO - Mutation Research Letters

JF - Mutation Research Letters

SN - 0027-5107

IS - 1-2

ER -

ID: 119639828