Microbiological evaluation of jejunal aspirates and faecal samples after oral administration of bifidobacteria and lactic acid bacteria
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Microbiological evaluation of jejunal aspirates and faecal samples after oral administration of bifidobacteria and lactic acid bacteria. / Nielsen, O H; Jørgensen, S; Pedersen, K; Justesen, T.
In: Journal of Applied Bacteriology, Vol. 76, No. 5, 05.1994, p. 469-74.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Microbiological evaluation of jejunal aspirates and faecal samples after oral administration of bifidobacteria and lactic acid bacteria
AU - Nielsen, O H
AU - Jørgensen, S
AU - Pedersen, K
AU - Justesen, T
PY - 1994/5
Y1 - 1994/5
N2 - A double-blind placebo controlled investigation was carried out to study the effect of peroral colonization. Human volunteers were given mixtures of bifidobacteria and lactic acid bacteria. Measurements were made over a 1 week treatment period and for another week after the end of the treatment. Two different bacteriological preparations were used, one consisted of Enterococcus faecium and Bifidobacterium longum (a total of 6.4 x 10(8) cfu d-1); the other consisted of Lactobacillus acidophilus, Bif. bifidum, Lact. delbrueckii ssp. bulgaricus, and Streptococcus thermophilus (a total of 9 x 10(9) cfu d-1). Together with a placebo preparation, they were given to 24 healthy controls (eight in each group). Microbiological examinations of jejunal aspirates showed that viable counts of most species were below the detection limit. However, the test preparation containing Ent. faecium and Bif. longum significantly reduced the anaerobe: aerobe ratio in faeces by a factor of three during treatment (P = 0.03), and increased it by a factor of 30 during the following week (P < 0.02). This study shows that peroral administration of certain bacterial cultures may affect the distal intestinal microflora.
AB - A double-blind placebo controlled investigation was carried out to study the effect of peroral colonization. Human volunteers were given mixtures of bifidobacteria and lactic acid bacteria. Measurements were made over a 1 week treatment period and for another week after the end of the treatment. Two different bacteriological preparations were used, one consisted of Enterococcus faecium and Bifidobacterium longum (a total of 6.4 x 10(8) cfu d-1); the other consisted of Lactobacillus acidophilus, Bif. bifidum, Lact. delbrueckii ssp. bulgaricus, and Streptococcus thermophilus (a total of 9 x 10(9) cfu d-1). Together with a placebo preparation, they were given to 24 healthy controls (eight in each group). Microbiological examinations of jejunal aspirates showed that viable counts of most species were below the detection limit. However, the test preparation containing Ent. faecium and Bif. longum significantly reduced the anaerobe: aerobe ratio in faeces by a factor of three during treatment (P = 0.03), and increased it by a factor of 30 during the following week (P < 0.02). This study shows that peroral administration of certain bacterial cultures may affect the distal intestinal microflora.
KW - Adult
KW - Bacteria
KW - Bifidobacterium
KW - Double-Blind Method
KW - Enterococcus faecium
KW - Feces/microbiology
KW - Female
KW - Humans
KW - Jejunum/microbiology
KW - Lactates/pharmacology
KW - Lactic Acid
KW - Lactobacillus
KW - Male
KW - Middle Aged
KW - Streptococcus
M3 - Journal article
C2 - 8005834
VL - 76
SP - 469
EP - 474
JO - Journal of Applied Bacteriology
JF - Journal of Applied Bacteriology
SN - 0021-8847
IS - 5
ER -
ID: 218727409