Metabolism of short-chain fatty acid propionate induces surface expression of NKG2D ligands on cancer cells
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Metabolism of short-chain fatty acid propionate induces surface expression of NKG2D ligands on cancer cells. / Høgh, Rikke Illum; Møller, Sofie Hedlund; Jepsen, Stine Dam; Mellergaard, Maiken; Lund, Astrid; Pejtersen, Mikala; Fitzner, Emil; Andresen, Lars; Skov, Søren.
In: FASEB Journal, Vol. 34, No. 11, 2020, p. 15531-15546.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Metabolism of short-chain fatty acid propionate induces surface expression of NKG2D ligands on cancer cells
AU - Høgh, Rikke Illum
AU - Møller, Sofie Hedlund
AU - Jepsen, Stine Dam
AU - Mellergaard, Maiken
AU - Lund, Astrid
AU - Pejtersen, Mikala
AU - Fitzner, Emil
AU - Andresen, Lars
AU - Skov, Søren
PY - 2020
Y1 - 2020
N2 - SCFAs are primarily produced in the colon by bacterial fermentation of nondigestible carbohydrates. Besides providing energy, SCFAs can suppress development of colon cancer. The mechanism, however, remains elusive. Here, we demonstrate that the SCFA propionate upregulates surface expression of the immune stimulatory NKG2D ligands, MICA/B by imposing metabolic changes in dividing cells. Propionate-mediated MICA/B expression did not rely on GPR41/GPR43 receptors but depended on functional mitochondria. By siRNA-directed knockdown, we could further link phosphoenolpyruvate carboxykinase (PEPCK), the rate-limiting enzyme in gluconeogenesis to propionate regulation of MICA/B expression. Moreover, knockdown of Rictor and specific mTOR inhibitors implicated mTORC2 activity with metabolic changes that control MICA/B expression. SCFAs are precursors to short-chain acyl-CoAs that are used for histone acylation thereby linking the metabolic state to chromatin structure and gene expression. Propionate increased the overall acetylation and propionylation and inhibition of lysine acetyltransferases (KATs) that are responsible for adding acyl-CoAs to histones reduced propionate-mediated MICA/B expression, suggesting that propionate-induced acylation increases MICA/B expression. Notably, propionate upregulated MICA/B surface expression on colon cancer cells in an acylation-dependent manner; however, the impact of mitochondrial metabolism on MICA/B expression was different in colon cancer cells compared with Jurkat cells, suggesting that continuous exposure to propionate in the colon may provide an enhanced capacity to metabolize propionate. Together, our findings support that propionate causes metabolic changes resulting in NKG2D ligand surface expression, which holds potential as an immune activating anticancer therapy.
AB - SCFAs are primarily produced in the colon by bacterial fermentation of nondigestible carbohydrates. Besides providing energy, SCFAs can suppress development of colon cancer. The mechanism, however, remains elusive. Here, we demonstrate that the SCFA propionate upregulates surface expression of the immune stimulatory NKG2D ligands, MICA/B by imposing metabolic changes in dividing cells. Propionate-mediated MICA/B expression did not rely on GPR41/GPR43 receptors but depended on functional mitochondria. By siRNA-directed knockdown, we could further link phosphoenolpyruvate carboxykinase (PEPCK), the rate-limiting enzyme in gluconeogenesis to propionate regulation of MICA/B expression. Moreover, knockdown of Rictor and specific mTOR inhibitors implicated mTORC2 activity with metabolic changes that control MICA/B expression. SCFAs are precursors to short-chain acyl-CoAs that are used for histone acylation thereby linking the metabolic state to chromatin structure and gene expression. Propionate increased the overall acetylation and propionylation and inhibition of lysine acetyltransferases (KATs) that are responsible for adding acyl-CoAs to histones reduced propionate-mediated MICA/B expression, suggesting that propionate-induced acylation increases MICA/B expression. Notably, propionate upregulated MICA/B surface expression on colon cancer cells in an acylation-dependent manner; however, the impact of mitochondrial metabolism on MICA/B expression was different in colon cancer cells compared with Jurkat cells, suggesting that continuous exposure to propionate in the colon may provide an enhanced capacity to metabolize propionate. Together, our findings support that propionate causes metabolic changes resulting in NKG2D ligand surface expression, which holds potential as an immune activating anticancer therapy.
KW - cancer
KW - immunometabolism
KW - NKG2D ligands
KW - short-chain fatty acids
U2 - 10.1096/fj.202000162R
DO - 10.1096/fj.202000162R
M3 - Journal article
C2 - 32996653
AN - SCOPUS:85091725393
VL - 34
SP - 15531
EP - 15546
JO - F A S E B Journal
JF - F A S E B Journal
SN - 0892-6638
IS - 11
ER -
ID: 249773162