Memory T helper cells identify patients with nickel, cobalt and chromium metal allergy
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Memory T helper cells identify patients with nickel, cobalt and chromium metal allergy. / Blom, Lars H; Elrefaii, Sali A; Zachariae, Claus; Thyssen, Jacob P; Poulsen, Lars K; Johansen, Jeanne D.
In: Contact Dermatitis, Vol. 85, No. 1, 2021, p. 7-16.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Memory T helper cells identify patients with nickel, cobalt and chromium metal allergy
AU - Blom, Lars H
AU - Elrefaii, Sali A
AU - Zachariae, Claus
AU - Thyssen, Jacob P
AU - Poulsen, Lars K
AU - Johansen, Jeanne D
N1 - This article is protected by copyright. All rights reserved.
PY - 2021
Y1 - 2021
N2 - BACKGROUND: Patch testing is the gold standard for identifying culprit allergens in allergic contact dermatitis; however, it's laborious and positive reactions are difficult to quantitate. Development of complementary in vitro tests is, therefore, of great importance.OBJECTIVES: This study aimed to improve the in vitro lymphocyte proliferation test (LPT) to detect allergic responses to nickel, cobalt and chromium.METHODS: Twenty-one metal-allergic patients with a positive patch test to nickel (16), cobalt (8) and chromium (3) and thirteen controls were included. All were tested by a flow cytometric LPT.RESULTS: Metal-reactive cells were identified as Th cells with high expression of the memory marker CD45RO. Skin-homing (CLA+ ) nickel-reactive memory Th (Thmemhi ) cells identified individuals with a positive patch test for nickel with 100 % sensitivity [81%,100%] and 92% specificity [67%,100%]. Moreover, cobalt-specific Thmemhi cells expressing CCR6 identified patients with a positive patch test for cobalt with 63% sensitivity [31%,86%] and 100% specificity [77%,100%]. In chromium allergic individuals, Chromium-reactive Thmemhi cells tended to increased CLA and CCR6 expression.CONCLUSION: Metal-reactive Th cells with high expression CD45RO and co-expression of CLA and CCR6 improved the LPT, making it an attractive supplement to the patch test. This article is protected by copyright. All rights reserved.
AB - BACKGROUND: Patch testing is the gold standard for identifying culprit allergens in allergic contact dermatitis; however, it's laborious and positive reactions are difficult to quantitate. Development of complementary in vitro tests is, therefore, of great importance.OBJECTIVES: This study aimed to improve the in vitro lymphocyte proliferation test (LPT) to detect allergic responses to nickel, cobalt and chromium.METHODS: Twenty-one metal-allergic patients with a positive patch test to nickel (16), cobalt (8) and chromium (3) and thirteen controls were included. All were tested by a flow cytometric LPT.RESULTS: Metal-reactive cells were identified as Th cells with high expression of the memory marker CD45RO. Skin-homing (CLA+ ) nickel-reactive memory Th (Thmemhi ) cells identified individuals with a positive patch test for nickel with 100 % sensitivity [81%,100%] and 92% specificity [67%,100%]. Moreover, cobalt-specific Thmemhi cells expressing CCR6 identified patients with a positive patch test for cobalt with 63% sensitivity [31%,86%] and 100% specificity [77%,100%]. In chromium allergic individuals, Chromium-reactive Thmemhi cells tended to increased CLA and CCR6 expression.CONCLUSION: Metal-reactive Th cells with high expression CD45RO and co-expression of CLA and CCR6 improved the LPT, making it an attractive supplement to the patch test. This article is protected by copyright. All rights reserved.
U2 - 10.1111/cod.13809
DO - 10.1111/cod.13809
M3 - Journal article
C2 - 33576045
VL - 85
SP - 7
EP - 16
JO - Contact Dermatitis
JF - Contact Dermatitis
SN - 0105-1873
IS - 1
ER -
ID: 256932876