Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies.

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Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies. / Couchman, J R; Ljubimov, A V.

In: Matrix (Stuttgart, Germany), Vol. 9, No. 4, 1989, p. 311-21.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Couchman, JR & Ljubimov, AV 1989, 'Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies.', Matrix (Stuttgart, Germany), vol. 9, no. 4, pp. 311-21.

APA

Couchman, J. R., & Ljubimov, A. V. (1989). Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies. Matrix (Stuttgart, Germany), 9(4), 311-21.

Vancouver

Couchman JR, Ljubimov AV. Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies. Matrix (Stuttgart, Germany). 1989;9(4):311-21.

Author

Couchman, J R ; Ljubimov, A V. / Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies. In: Matrix (Stuttgart, Germany). 1989 ; Vol. 9, No. 4. pp. 311-21.

Bibtex

@article{f7bd30c0598011dd8d9f000ea68e967b,
title = "Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies.",
abstract = "A panel of nine monoclonal antibodies has been characterized, all of which have reactivity with the core protein of a large heparan sulfate proteoglycan derived from the murine EHS tumor matrix. These rat monoclonal antibodies stained mouse basement membranes intensely, including those of all muscle, endothelia, peripheral nerve fibers and epithelia so far examined. In addition, two of the monoclonal antibodies show cross-species reactivity, staining bovine and human basement membranes, and immunoprecipitating proteoglycans from human endothelial cell cultures. These antibodies do not, however, cross-react with avian tissues. These results show the ubiquitous distribution of a heparan sulfate proteoglycan in mammalian tissues, which will be useful in vitro and in vivo for studies on the biology of basement membrane proteoglycans and investigations of possible roles of these molecules in human disease processes.",
author = "Couchman, {J R} and Ljubimov, {A V}",
note = "Keywords: Animals; Antibodies, Monoclonal; Antibody Specificity; Basement Membrane; Cattle; Epitopes; Extracellular Matrix; Fluorescent Antibody Technique; Heparin; Histocytochemistry; Humans; Kidney; Mice; Muscles; Proteoglycans; Rats; Tissue Distribution; Umbilical Veins",
year = "1989",
language = "English",
volume = "9",
pages = "311--21",
journal = "Matrix",
issn = "0934-8832",
publisher = "Fischer Medical Publications",
number = "4",

}

RIS

TY - JOUR

T1 - Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies.

AU - Couchman, J R

AU - Ljubimov, A V

N1 - Keywords: Animals; Antibodies, Monoclonal; Antibody Specificity; Basement Membrane; Cattle; Epitopes; Extracellular Matrix; Fluorescent Antibody Technique; Heparin; Histocytochemistry; Humans; Kidney; Mice; Muscles; Proteoglycans; Rats; Tissue Distribution; Umbilical Veins

PY - 1989

Y1 - 1989

N2 - A panel of nine monoclonal antibodies has been characterized, all of which have reactivity with the core protein of a large heparan sulfate proteoglycan derived from the murine EHS tumor matrix. These rat monoclonal antibodies stained mouse basement membranes intensely, including those of all muscle, endothelia, peripheral nerve fibers and epithelia so far examined. In addition, two of the monoclonal antibodies show cross-species reactivity, staining bovine and human basement membranes, and immunoprecipitating proteoglycans from human endothelial cell cultures. These antibodies do not, however, cross-react with avian tissues. These results show the ubiquitous distribution of a heparan sulfate proteoglycan in mammalian tissues, which will be useful in vitro and in vivo for studies on the biology of basement membrane proteoglycans and investigations of possible roles of these molecules in human disease processes.

AB - A panel of nine monoclonal antibodies has been characterized, all of which have reactivity with the core protein of a large heparan sulfate proteoglycan derived from the murine EHS tumor matrix. These rat monoclonal antibodies stained mouse basement membranes intensely, including those of all muscle, endothelia, peripheral nerve fibers and epithelia so far examined. In addition, two of the monoclonal antibodies show cross-species reactivity, staining bovine and human basement membranes, and immunoprecipitating proteoglycans from human endothelial cell cultures. These antibodies do not, however, cross-react with avian tissues. These results show the ubiquitous distribution of a heparan sulfate proteoglycan in mammalian tissues, which will be useful in vitro and in vivo for studies on the biology of basement membrane proteoglycans and investigations of possible roles of these molecules in human disease processes.

M3 - Journal article

C2 - 2477673

VL - 9

SP - 311

EP - 321

JO - Matrix

JF - Matrix

SN - 0934-8832

IS - 4

ER -

ID: 5167215