LPS from Porphyromonas gingivalis increases the sensitivity of contractile response mediated by endothelin-B (ET(B)) receptors in cultured endothelium-intact rat coronary arteries

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

LPS from Porphyromonas gingivalis increases the sensitivity of contractile response mediated by endothelin-B (ET(B)) receptors in cultured endothelium-intact rat coronary arteries. / Ghorbani, Bahareh; Holmstrup, Palle; Edvinsson, Lars; Kristiansen, Kim A; Sheykhzade, Majid; Ghorbani, Bahareh; Holmstrup, Palle; Edvinsson, Lars; Kristiansen, Kim A; Sheykhzade, Majid.

In: Vascular Pharmacology, Vol. 53, No. 5-6, 2010, p. 250-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ghorbani, B, Holmstrup, P, Edvinsson, L, Kristiansen, KA, Sheykhzade, M, Ghorbani, B, Holmstrup, P, Edvinsson, L, Kristiansen, KA & Sheykhzade, M 2010, 'LPS from Porphyromonas gingivalis increases the sensitivity of contractile response mediated by endothelin-B (ET(B)) receptors in cultured endothelium-intact rat coronary arteries', Vascular Pharmacology, vol. 53, no. 5-6, pp. 250-7. https://doi.org/10.1016/j.vph.2010.09.006, https://doi.org/10.1016/j.vph.2010.09.006

APA

Ghorbani, B., Holmstrup, P., Edvinsson, L., Kristiansen, K. A., Sheykhzade, M., Ghorbani, B., Holmstrup, P., Edvinsson, L., Kristiansen, K. A., & Sheykhzade, M. (2010). LPS from Porphyromonas gingivalis increases the sensitivity of contractile response mediated by endothelin-B (ET(B)) receptors in cultured endothelium-intact rat coronary arteries. Vascular Pharmacology, 53(5-6), 250-7. https://doi.org/10.1016/j.vph.2010.09.006, https://doi.org/10.1016/j.vph.2010.09.006

Vancouver

Ghorbani B, Holmstrup P, Edvinsson L, Kristiansen KA, Sheykhzade M, Ghorbani B et al. LPS from Porphyromonas gingivalis increases the sensitivity of contractile response mediated by endothelin-B (ET(B)) receptors in cultured endothelium-intact rat coronary arteries. Vascular Pharmacology. 2010;53(5-6):250-7. https://doi.org/10.1016/j.vph.2010.09.006, https://doi.org/10.1016/j.vph.2010.09.006

Author

Ghorbani, Bahareh ; Holmstrup, Palle ; Edvinsson, Lars ; Kristiansen, Kim A ; Sheykhzade, Majid ; Ghorbani, Bahareh ; Holmstrup, Palle ; Edvinsson, Lars ; Kristiansen, Kim A ; Sheykhzade, Majid. / LPS from Porphyromonas gingivalis increases the sensitivity of contractile response mediated by endothelin-B (ET(B)) receptors in cultured endothelium-intact rat coronary arteries. In: Vascular Pharmacology. 2010 ; Vol. 53, No. 5-6. pp. 250-7.

Bibtex

@article{6d752880d84211df825b000ea68e967b,
title = "LPS from Porphyromonas gingivalis increases the sensitivity of contractile response mediated by endothelin-B (ET(B)) receptors in cultured endothelium-intact rat coronary arteries",
abstract = "The purpose of our study was to examine if lipopolysaccharide (LPS) from Porphyromonas gingivalis (P.g.) modifies the vasomotor responses to Endothelin-1 (ET-1) and Sarafotoxin 6c (S6c) in rat coronary arteries. The arteries were studied directly or following organ culture for 24h in absence and presence of 2.5EU/ml LPS. The contractile responses of coronary arteries were investigated by using the selective ETB receptor agonist S6c (1 pM-0.3µM) and ET-1 (1 pM-0.3µM). The functional studies demonstrated an augmented contractile response only to S6c in isolated rat coronary arteries after organ culture (with or without LPS). These contractile responses by S6c were blocked by the selective ETB receptor antagonist BQ788 in both vessel groups. The augmented contractile response to S6c was supported by immunohistochemistry, where a significant increase in fluorescence intensity for ETB receptors in smooth muscle cells was observed after organ culture. The presence of LPS in the culture medium significantly increased the sensitivity of endothelium-intact coronary artery to S6c as compared to endothelium-denuded segments. Our results showed a significant increase in both ETB receptor protein levels and S6c-induced maximal contraction in coronary arteries upon 24h of organ culture, which was further sensitized by LPS.",
author = "Bahareh Ghorbani and Palle Holmstrup and Lars Edvinsson and Kristiansen, {Kim A} and Majid Sheykhzade and Bahareh Ghorbani and Palle Holmstrup and Lars Edvinsson and Kristiansen, {Kim A} and Majid Sheykhzade",
note = "Copyright {\textcopyright} 2010. Published by Elsevier Inc.",
year = "2010",
doi = "10.1016/j.vph.2010.09.006",
language = "English",
volume = "53",
pages = "250--7",
journal = "Vascular Pharmacology",
issn = "1537-1891",
publisher = "Elsevier",
number = "5-6",

}

RIS

TY - JOUR

T1 - LPS from Porphyromonas gingivalis increases the sensitivity of contractile response mediated by endothelin-B (ET(B)) receptors in cultured endothelium-intact rat coronary arteries

AU - Ghorbani, Bahareh

AU - Holmstrup, Palle

AU - Edvinsson, Lars

AU - Kristiansen, Kim A

AU - Sheykhzade, Majid

AU - Ghorbani, Bahareh

AU - Holmstrup, Palle

AU - Edvinsson, Lars

AU - Kristiansen, Kim A

AU - Sheykhzade, Majid

N1 - Copyright © 2010. Published by Elsevier Inc.

PY - 2010

Y1 - 2010

N2 - The purpose of our study was to examine if lipopolysaccharide (LPS) from Porphyromonas gingivalis (P.g.) modifies the vasomotor responses to Endothelin-1 (ET-1) and Sarafotoxin 6c (S6c) in rat coronary arteries. The arteries were studied directly or following organ culture for 24h in absence and presence of 2.5EU/ml LPS. The contractile responses of coronary arteries were investigated by using the selective ETB receptor agonist S6c (1 pM-0.3µM) and ET-1 (1 pM-0.3µM). The functional studies demonstrated an augmented contractile response only to S6c in isolated rat coronary arteries after organ culture (with or without LPS). These contractile responses by S6c were blocked by the selective ETB receptor antagonist BQ788 in both vessel groups. The augmented contractile response to S6c was supported by immunohistochemistry, where a significant increase in fluorescence intensity for ETB receptors in smooth muscle cells was observed after organ culture. The presence of LPS in the culture medium significantly increased the sensitivity of endothelium-intact coronary artery to S6c as compared to endothelium-denuded segments. Our results showed a significant increase in both ETB receptor protein levels and S6c-induced maximal contraction in coronary arteries upon 24h of organ culture, which was further sensitized by LPS.

AB - The purpose of our study was to examine if lipopolysaccharide (LPS) from Porphyromonas gingivalis (P.g.) modifies the vasomotor responses to Endothelin-1 (ET-1) and Sarafotoxin 6c (S6c) in rat coronary arteries. The arteries were studied directly or following organ culture for 24h in absence and presence of 2.5EU/ml LPS. The contractile responses of coronary arteries were investigated by using the selective ETB receptor agonist S6c (1 pM-0.3µM) and ET-1 (1 pM-0.3µM). The functional studies demonstrated an augmented contractile response only to S6c in isolated rat coronary arteries after organ culture (with or without LPS). These contractile responses by S6c were blocked by the selective ETB receptor antagonist BQ788 in both vessel groups. The augmented contractile response to S6c was supported by immunohistochemistry, where a significant increase in fluorescence intensity for ETB receptors in smooth muscle cells was observed after organ culture. The presence of LPS in the culture medium significantly increased the sensitivity of endothelium-intact coronary artery to S6c as compared to endothelium-denuded segments. Our results showed a significant increase in both ETB receptor protein levels and S6c-induced maximal contraction in coronary arteries upon 24h of organ culture, which was further sensitized by LPS.

U2 - 10.1016/j.vph.2010.09.006

DO - 10.1016/j.vph.2010.09.006

M3 - Journal article

C2 - 20888431

VL - 53

SP - 250

EP - 257

JO - Vascular Pharmacology

JF - Vascular Pharmacology

SN - 1537-1891

IS - 5-6

ER -

ID: 22524337