Lower limb ischaemia and reperfusion injury in healthy volunteers measured by oxidative and inflammatory biomarkers
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Lower limb ischaemia and reperfusion injury in healthy volunteers measured by oxidative and inflammatory biomarkers. / Halladin, N. L.; Busch, Sarah Victoria Ekeløf; Alamili, M.; Bendtzen, K.; Lykkesfeldt, Jens; Rosenberg, Jacob; Gögenür, Ismayil.
In: Perfusion, Vol. 30, No. 1, 10.04.2015, p. 64-70.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Lower limb ischaemia and reperfusion injury in healthy volunteers measured by oxidative and inflammatory biomarkers
AU - Halladin, N. L.
AU - Busch, Sarah Victoria Ekeløf
AU - Alamili, M.
AU - Bendtzen, K.
AU - Lykkesfeldt, Jens
AU - Rosenberg, Jacob
AU - Gögenür, Ismayil
PY - 2015/4/10
Y1 - 2015/4/10
N2 - OBJECTIVE: Ischaemia-reperfusion (IR) injury is partly caused by the release of reactive oxygen species and cytokines and may result in remote organ injury. Surgical patients are exposed to surgical stress and anaesthesia, both of which can influence the IR response. An IR model without these interfering factors of surgery is, therefore, useful to test the potential of antioxidant and cytokine-modulatory treatments.The aim of this study was to characterize a human ischaemia-reperfusion model with respect to oxidative and inflammatory biomarkers.MATERIALS AND METHODS: Ten male volunteers were exposed to 20 minutes of lower limb ischaemia. Muscle biopsies and blood samples were taken at baseline and 5, 15, 30, 60 and 90 minutes after tourniquet release and analysed for malondialdehyde (MDA), ascorbic acid, dehydroascorbic acid, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-1 receptor antagonist (IL-1Ra), IL-6, IL-10, TNF-receptor (TNF-R)I, TNF-RII and YKL-40.RESULTS: We found no significant increase in MDA in the muscle biopsies after reperfusion. Plasma levels of oxidative and pro- and anti-inflammatory parameters showed no significant differences between baseline and after reperfusion at any sampling time.CONCLUSION: Twenty minutes of lower limb ischaemia does not result in an ischaemia-reperfusion injury in healthy volunteers, measurable by oxidative and pro- and anti-inflammatory biomarkers in muscle biopsies and in the systemic circulation.
AB - OBJECTIVE: Ischaemia-reperfusion (IR) injury is partly caused by the release of reactive oxygen species and cytokines and may result in remote organ injury. Surgical patients are exposed to surgical stress and anaesthesia, both of which can influence the IR response. An IR model without these interfering factors of surgery is, therefore, useful to test the potential of antioxidant and cytokine-modulatory treatments.The aim of this study was to characterize a human ischaemia-reperfusion model with respect to oxidative and inflammatory biomarkers.MATERIALS AND METHODS: Ten male volunteers were exposed to 20 minutes of lower limb ischaemia. Muscle biopsies and blood samples were taken at baseline and 5, 15, 30, 60 and 90 minutes after tourniquet release and analysed for malondialdehyde (MDA), ascorbic acid, dehydroascorbic acid, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-1 receptor antagonist (IL-1Ra), IL-6, IL-10, TNF-receptor (TNF-R)I, TNF-RII and YKL-40.RESULTS: We found no significant increase in MDA in the muscle biopsies after reperfusion. Plasma levels of oxidative and pro- and anti-inflammatory parameters showed no significant differences between baseline and after reperfusion at any sampling time.CONCLUSION: Twenty minutes of lower limb ischaemia does not result in an ischaemia-reperfusion injury in healthy volunteers, measurable by oxidative and pro- and anti-inflammatory biomarkers in muscle biopsies and in the systemic circulation.
U2 - 10.1177/0267659114530769
DO - 10.1177/0267659114530769
M3 - Journal article
C2 - 24722849
VL - 30
SP - 64
EP - 70
JO - Perfusion (United Kingdom)
JF - Perfusion (United Kingdom)
SN - 0267-6591
IS - 1
ER -
ID: 124843811