Low risk for transmission of zoonotic Giardia duodenalis from dogs to humans in rural Cambodia
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Low risk for transmission of zoonotic Giardia duodenalis from dogs to humans in rural Cambodia. / Inpankaew, Tawin; Schär, Fabian; Odermatt, Peter; Dalsgaard, Anders; Chimnoi, Wissanuwat; Khieu, Virak; Muth, Sinuon; Traub, Rebecca J.
In: Parasites & Vectors, Vol. 7, 412, 2014.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Low risk for transmission of zoonotic Giardia duodenalis from dogs to humans in rural Cambodia
AU - Inpankaew, Tawin
AU - Schär, Fabian
AU - Odermatt, Peter
AU - Dalsgaard, Anders
AU - Chimnoi, Wissanuwat
AU - Khieu, Virak
AU - Muth, Sinuon
AU - Traub, Rebecca J.
PY - 2014
Y1 - 2014
N2 - BACKGROUND: A number of epidemiological studies have demonstrated Giardia as prevalent in both humans and dogs worldwide and have postulated the occurrence of anthroponotic, zoonotic and animal-specific cycles of transmission, which may be geographically and regionally unique in its epidemiology. The aim of this study was to utilise molecular tools to determine the prevalence and compare genotypes of Giardia duodenalis infecting humans and dogs living in a previously identified Giardia-endemic village in rural Cambodia in order to ascertain zoonotic transmission risk.FINDINGS: The prevalence of G. duodenalis in humans and dogs was 18.3% (40/218) and 10.6% (10/94) by PCR, respectively. Molecular characterisation of the small subunit of ribosomal RNA (SSU rRNA) gene, triose phosphate isomerase (TPI) gene and sub-assemblage characterisation of the glutamate dehydrogenase (gdh) gene placed 27.5% (11/40) of Giardia positive humans into assemblage AII and 72.5% (29/40) into assemblage BIII of G. duodenalis. In dogs, 20.0% (2/10) of Giardia-positive samples were characterised as G. duodenalis assemblage BIII, 40.0% (4/10) as assemblage C and 40.0% (4/10) as mix infection between assemblage C and D.CONCLUSION: Overall, just over 2% of dogs harboured potentially zoonotic assemblages of G. duodenalis in the studied communities and hence pose a minimal zoonotic risk for the transmission of Giardia to humans.
AB - BACKGROUND: A number of epidemiological studies have demonstrated Giardia as prevalent in both humans and dogs worldwide and have postulated the occurrence of anthroponotic, zoonotic and animal-specific cycles of transmission, which may be geographically and regionally unique in its epidemiology. The aim of this study was to utilise molecular tools to determine the prevalence and compare genotypes of Giardia duodenalis infecting humans and dogs living in a previously identified Giardia-endemic village in rural Cambodia in order to ascertain zoonotic transmission risk.FINDINGS: The prevalence of G. duodenalis in humans and dogs was 18.3% (40/218) and 10.6% (10/94) by PCR, respectively. Molecular characterisation of the small subunit of ribosomal RNA (SSU rRNA) gene, triose phosphate isomerase (TPI) gene and sub-assemblage characterisation of the glutamate dehydrogenase (gdh) gene placed 27.5% (11/40) of Giardia positive humans into assemblage AII and 72.5% (29/40) into assemblage BIII of G. duodenalis. In dogs, 20.0% (2/10) of Giardia-positive samples were characterised as G. duodenalis assemblage BIII, 40.0% (4/10) as assemblage C and 40.0% (4/10) as mix infection between assemblage C and D.CONCLUSION: Overall, just over 2% of dogs harboured potentially zoonotic assemblages of G. duodenalis in the studied communities and hence pose a minimal zoonotic risk for the transmission of Giardia to humans.
KW - Faculty of Health and Medical Sciences
KW - Giardia duodenalis
KW - Humans
KW - Dogs
KW - Zoonosis
KW - Cambodia
U2 - 10.1186/1756-3305-7-412
DO - 10.1186/1756-3305-7-412
M3 - Journal article
C2 - 25175607
VL - 7
JO - Parasites & Vectors
JF - Parasites & Vectors
SN - 1756-3305
M1 - 412
ER -
ID: 123734640