Loss of Adgra3 causes obstructive azoospermia with high penetrance in male mice

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Loss of Adgra3 causes obstructive azoospermia with high penetrance in male mice. / Nybo, Maja L.; Kvam, Jone M.; Nielsen, John E.; Frederiksen, Hanne; Spiess, Katja; Jensen, Kristian H. R.; Gadgaard, Sarina; Walser, Anna L. S.; Thomsen, Jesper S.; Cowin, Pamela; Juul, Anders; Jensen, Martin B.; Rosenkilde, Mette M.

In: FASEB Journal, Vol. 37, No. 2, 22781, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nybo, ML, Kvam, JM, Nielsen, JE, Frederiksen, H, Spiess, K, Jensen, KHR, Gadgaard, S, Walser, ALS, Thomsen, JS, Cowin, P, Juul, A, Jensen, MB & Rosenkilde, MM 2023, 'Loss of Adgra3 causes obstructive azoospermia with high penetrance in male mice', FASEB Journal, vol. 37, no. 2, 22781. https://doi.org/10.1096/fj.202200762RR

APA

Nybo, M. L., Kvam, J. M., Nielsen, J. E., Frederiksen, H., Spiess, K., Jensen, K. H. R., Gadgaard, S., Walser, A. L. S., Thomsen, J. S., Cowin, P., Juul, A., Jensen, M. B., & Rosenkilde, M. M. (2023). Loss of Adgra3 causes obstructive azoospermia with high penetrance in male mice. FASEB Journal, 37(2), [22781]. https://doi.org/10.1096/fj.202200762RR

Vancouver

Nybo ML, Kvam JM, Nielsen JE, Frederiksen H, Spiess K, Jensen KHR et al. Loss of Adgra3 causes obstructive azoospermia with high penetrance in male mice. FASEB Journal. 2023;37(2). 22781. https://doi.org/10.1096/fj.202200762RR

Author

Nybo, Maja L. ; Kvam, Jone M. ; Nielsen, John E. ; Frederiksen, Hanne ; Spiess, Katja ; Jensen, Kristian H. R. ; Gadgaard, Sarina ; Walser, Anna L. S. ; Thomsen, Jesper S. ; Cowin, Pamela ; Juul, Anders ; Jensen, Martin B. ; Rosenkilde, Mette M. / Loss of Adgra3 causes obstructive azoospermia with high penetrance in male mice. In: FASEB Journal. 2023 ; Vol. 37, No. 2.

Bibtex

@article{b0e792711ac8470882c78008b7857855,
title = "Loss of Adgra3 causes obstructive azoospermia with high penetrance in male mice",
abstract = "The adhesion receptor ADGRA3 (GPR125) is a known spermatogonial stem cell marker, but its impact on male reproduction and fertility has not been examined. Using a mouse model lacking Adgra3 (Adgra3(-/-)), we show that 55% of the male mice are infertile from puberty despite having normal spermatogenesis and epididymal sperm count. Instead, male mice lacking Adgra3 exhibited decreased estrogen receptor alpha expression and transient dilation of the epididymis. Combined with an increased estradiol production, this indicates a post-pubertal hormonal imbalance and fluid retention. Dye injection revealed a blockage between the ejaculatory duct and the urethra, which is rare in mice suffering from infertility, thereby mimicking the etiologies of obstructive azoospermia found in human male infertility. To summarize, male reproductive tract development is dependent on ADGRA3 function that in concert with estrogen signaling may influence fluid handling during sperm maturation and storage.",
keywords = "adhesion G protein-coupled receptor, ejaculatory duct, ejaculatory duct obstruction, estrogen, GPR125, infertility, seminal vesicles, Wnt signaling, EFFERENT DUCTULES, UROGENITAL SINUS, MALE-INFERTILITY, BETA-CATENIN, EXPRESSION, ESTROGEN, MOUSE, EPIDIDYMIS, EPITHELIUM, RAT",
author = "Nybo, {Maja L.} and Kvam, {Jone M.} and Nielsen, {John E.} and Hanne Frederiksen and Katja Spiess and Jensen, {Kristian H. R.} and Sarina Gadgaard and Walser, {Anna L. S.} and Thomsen, {Jesper S.} and Pamela Cowin and Anders Juul and Jensen, {Martin B.} and Rosenkilde, {Mette M.}",
year = "2023",
doi = "10.1096/fj.202200762RR",
language = "English",
volume = "37",
journal = "F A S E B Journal",
issn = "0892-6638",
publisher = "Federation of American Societies for Experimental Biology",
number = "2",

}

RIS

TY - JOUR

T1 - Loss of Adgra3 causes obstructive azoospermia with high penetrance in male mice

AU - Nybo, Maja L.

AU - Kvam, Jone M.

AU - Nielsen, John E.

AU - Frederiksen, Hanne

AU - Spiess, Katja

AU - Jensen, Kristian H. R.

AU - Gadgaard, Sarina

AU - Walser, Anna L. S.

AU - Thomsen, Jesper S.

AU - Cowin, Pamela

AU - Juul, Anders

AU - Jensen, Martin B.

AU - Rosenkilde, Mette M.

PY - 2023

Y1 - 2023

N2 - The adhesion receptor ADGRA3 (GPR125) is a known spermatogonial stem cell marker, but its impact on male reproduction and fertility has not been examined. Using a mouse model lacking Adgra3 (Adgra3(-/-)), we show that 55% of the male mice are infertile from puberty despite having normal spermatogenesis and epididymal sperm count. Instead, male mice lacking Adgra3 exhibited decreased estrogen receptor alpha expression and transient dilation of the epididymis. Combined with an increased estradiol production, this indicates a post-pubertal hormonal imbalance and fluid retention. Dye injection revealed a blockage between the ejaculatory duct and the urethra, which is rare in mice suffering from infertility, thereby mimicking the etiologies of obstructive azoospermia found in human male infertility. To summarize, male reproductive tract development is dependent on ADGRA3 function that in concert with estrogen signaling may influence fluid handling during sperm maturation and storage.

AB - The adhesion receptor ADGRA3 (GPR125) is a known spermatogonial stem cell marker, but its impact on male reproduction and fertility has not been examined. Using a mouse model lacking Adgra3 (Adgra3(-/-)), we show that 55% of the male mice are infertile from puberty despite having normal spermatogenesis and epididymal sperm count. Instead, male mice lacking Adgra3 exhibited decreased estrogen receptor alpha expression and transient dilation of the epididymis. Combined with an increased estradiol production, this indicates a post-pubertal hormonal imbalance and fluid retention. Dye injection revealed a blockage between the ejaculatory duct and the urethra, which is rare in mice suffering from infertility, thereby mimicking the etiologies of obstructive azoospermia found in human male infertility. To summarize, male reproductive tract development is dependent on ADGRA3 function that in concert with estrogen signaling may influence fluid handling during sperm maturation and storage.

KW - adhesion G protein-coupled receptor

KW - ejaculatory duct

KW - ejaculatory duct obstruction

KW - estrogen

KW - GPR125

KW - infertility

KW - seminal vesicles

KW - Wnt signaling

KW - EFFERENT DUCTULES

KW - UROGENITAL SINUS

KW - MALE-INFERTILITY

KW - BETA-CATENIN

KW - EXPRESSION

KW - ESTROGEN

KW - MOUSE

KW - EPIDIDYMIS

KW - EPITHELIUM

KW - RAT

U2 - 10.1096/fj.202200762RR

DO - 10.1096/fj.202200762RR

M3 - Journal article

C2 - 36688818

VL - 37

JO - F A S E B Journal

JF - F A S E B Journal

SN - 0892-6638

IS - 2

M1 - 22781

ER -

ID: 335688773