Long-term conservation tillage with reduced nitrogen fertilization intensity can improve winter wheat health via positive plant–microorganism feedback in the rhizosphere

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  • Jan Helge Behr
  • Theresa Kuhl-Nagel
  • Loreen Sommermann
  • Narges Moradtalab
  • Soumitra Paul Chowdhury
  • Michael Schloter
  • Saskia Windisch
  • Ingo Schellenberg
  • Maccario, Lorrie
  • Sørensen, Søren Johannes
  • Michael Rothballer
  • Joerg Geistlinger
  • Kornelia Smalla
  • Uwe Ludewig
  • Günter Neumann
  • Rita Grosch
  • Doreen Babin

Microbiome-based solutions are regarded key for sustainable agroecosystems. However, it is unclear how agricultural practices affect the rhizosphere microbiome, plant–microorganism interactions and crop performance under field conditions. Therefore, we installed root observation windows in a winter wheat field cultivated either under long-term mouldboard plough (MP) or cultivator tillage (CT). Each tillage practice was also compared at two nitrogen (N) fertilization intensities, intensive (recommended N-supply with pesticides/growth regulators) or extensive (reduced N-supply, no fungicides/growth regulators). Shoot biomass, root exudates and rhizosphere metabolites, physiological stress indicators, and gene expression were analyzed together with the rhizosphere microbiome (bacterial/archaeal 16S rRNA gene, fungal ITS amplicon, and shotgun metagenome sequencing) shortly before flowering. Compared to MP, the rhizosphere of CT winter wheat contained more primary and secondary metabolites, especially benzoxazinoid derivatives. Potential copiotrophic and plant-beneficial taxa (e.g. Bacillus, Devosia, and Trichoderma) as well as functional genes (e.g. siderophore production, trehalose synthase, and ACC deaminase) were enriched in the CT rhizosphere, suggesting that tillage affected belowground plant–microorganism interactions. In addition, physiological stress markers were suppressed in CT winter wheat compared to MP. In summary, tillage practice was a major driver of crop performance, root deposits, and rhizosphere microbiome interactions, while the N-fertilization intensity was also relevant, but less important.

Original languageEnglish
Article numberfiae003
JournalFEMS Microbiology Ecology
Issue number2
Number of pages18
Publication statusPublished - 2024

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© 2024 Oxford University Press. All rights reserved.

    Research areas

  • 16S rRNA gene, ITS Illumina amplicon sequencing, mineral fertilization, root exudates, shotgun metagenome sequencing, sustainable agriculture

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