lncRNA Panct1 Maintains Mouse Embryonic Stem Cell Identity by Regulating TOBF1 Recruitment to Oct-Sox Sequences in Early G1

Research output: Contribution to journalJournal articlepeer-review

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lncRNA Panct1 Maintains Mouse Embryonic Stem Cell Identity by Regulating TOBF1 Recruitment to Oct-Sox Sequences in Early G1. / Chakraborty, Debojyoti; Paszkowski-Rogacz, Maciej; Berger, Nicolas; Ding, Li; Mircetic, Jovan; Fu, Jun; Iesmantavicius, Vytautas; Choudhary, Chunaram; Anastassiadis, Konstantinos; Stewart, A Francis; Buchholz, Frank.

In: Cell Reports, Vol. 21, No. 11, 12.12.2017, p. 3012-3021.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Chakraborty, D, Paszkowski-Rogacz, M, Berger, N, Ding, L, Mircetic, J, Fu, J, Iesmantavicius, V, Choudhary, C, Anastassiadis, K, Stewart, AF & Buchholz, F 2017, 'lncRNA Panct1 Maintains Mouse Embryonic Stem Cell Identity by Regulating TOBF1 Recruitment to Oct-Sox Sequences in Early G1', Cell Reports, vol. 21, no. 11, pp. 3012-3021. https://doi.org/10.1016/j.celrep.2017.11.045

APA

Chakraborty, D., Paszkowski-Rogacz, M., Berger, N., Ding, L., Mircetic, J., Fu, J., Iesmantavicius, V., Choudhary, C., Anastassiadis, K., Stewart, A. F., & Buchholz, F. (2017). lncRNA Panct1 Maintains Mouse Embryonic Stem Cell Identity by Regulating TOBF1 Recruitment to Oct-Sox Sequences in Early G1. Cell Reports, 21(11), 3012-3021. https://doi.org/10.1016/j.celrep.2017.11.045

Vancouver

Chakraborty D, Paszkowski-Rogacz M, Berger N, Ding L, Mircetic J, Fu J et al. lncRNA Panct1 Maintains Mouse Embryonic Stem Cell Identity by Regulating TOBF1 Recruitment to Oct-Sox Sequences in Early G1. Cell Reports. 2017 Dec 12;21(11):3012-3021. https://doi.org/10.1016/j.celrep.2017.11.045

Author

Chakraborty, Debojyoti ; Paszkowski-Rogacz, Maciej ; Berger, Nicolas ; Ding, Li ; Mircetic, Jovan ; Fu, Jun ; Iesmantavicius, Vytautas ; Choudhary, Chunaram ; Anastassiadis, Konstantinos ; Stewart, A Francis ; Buchholz, Frank. / lncRNA Panct1 Maintains Mouse Embryonic Stem Cell Identity by Regulating TOBF1 Recruitment to Oct-Sox Sequences in Early G1. In: Cell Reports. 2017 ; Vol. 21, No. 11. pp. 3012-3021.

Bibtex

@article{cf557d7f190a449f8a742533af38ec56,
title = "lncRNA Panct1 Maintains Mouse Embryonic Stem Cell Identity by Regulating TOBF1 Recruitment to Oct-Sox Sequences in Early G1",
abstract = "Long noncoding RNAs (lncRNAs) have been implicated in diverse biological processes, including embryonic stem cell (ESC) maintenance. However, their functional mechanisms remain largely undefined. Here, we show that the lncRNA Panct1 regulates the transient recruitment of a putative X-chromosome-encoded protein A830080D01Rik, hereafter referred to as transient octamer binding factor 1 (TOBF1), to genomic sites resembling the canonical Oct-Sox motif. TOBF1 physically interacts with Panct1 and exhibits a cell-cycle-specific punctate localization in ESCs. At the chromatin level, this correlates with its recruitment to promoters of pluripotency genes. Strikingly, mutating an octamer-like motif in Panct1 RNA abrogates the strength of TOBF1 localization and recruitment to its targets. Taken together, our data reveal a tightly controlled spatial and temporal pattern of lncRNA-mediated gene regulation in a cell-cycle-dependent manner and suggest that lncRNAs might function as barcodes for identifying genomic addresses for maintaining cellular states.",
keywords = "Journal Article",
author = "Debojyoti Chakraborty and Maciej Paszkowski-Rogacz and Nicolas Berger and Li Ding and Jovan Mircetic and Jun Fu and Vytautas Iesmantavicius and Chunaram Choudhary and Konstantinos Anastassiadis and Stewart, {A Francis} and Frank Buchholz",
note = "Copyright {\textcopyright} 2017 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2017",
month = dec,
day = "12",
doi = "10.1016/j.celrep.2017.11.045",
language = "English",
volume = "21",
pages = "3012--3021",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "11",

}

RIS

TY - JOUR

T1 - lncRNA Panct1 Maintains Mouse Embryonic Stem Cell Identity by Regulating TOBF1 Recruitment to Oct-Sox Sequences in Early G1

AU - Chakraborty, Debojyoti

AU - Paszkowski-Rogacz, Maciej

AU - Berger, Nicolas

AU - Ding, Li

AU - Mircetic, Jovan

AU - Fu, Jun

AU - Iesmantavicius, Vytautas

AU - Choudhary, Chunaram

AU - Anastassiadis, Konstantinos

AU - Stewart, A Francis

AU - Buchholz, Frank

N1 - Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2017/12/12

Y1 - 2017/12/12

N2 - Long noncoding RNAs (lncRNAs) have been implicated in diverse biological processes, including embryonic stem cell (ESC) maintenance. However, their functional mechanisms remain largely undefined. Here, we show that the lncRNA Panct1 regulates the transient recruitment of a putative X-chromosome-encoded protein A830080D01Rik, hereafter referred to as transient octamer binding factor 1 (TOBF1), to genomic sites resembling the canonical Oct-Sox motif. TOBF1 physically interacts with Panct1 and exhibits a cell-cycle-specific punctate localization in ESCs. At the chromatin level, this correlates with its recruitment to promoters of pluripotency genes. Strikingly, mutating an octamer-like motif in Panct1 RNA abrogates the strength of TOBF1 localization and recruitment to its targets. Taken together, our data reveal a tightly controlled spatial and temporal pattern of lncRNA-mediated gene regulation in a cell-cycle-dependent manner and suggest that lncRNAs might function as barcodes for identifying genomic addresses for maintaining cellular states.

AB - Long noncoding RNAs (lncRNAs) have been implicated in diverse biological processes, including embryonic stem cell (ESC) maintenance. However, their functional mechanisms remain largely undefined. Here, we show that the lncRNA Panct1 regulates the transient recruitment of a putative X-chromosome-encoded protein A830080D01Rik, hereafter referred to as transient octamer binding factor 1 (TOBF1), to genomic sites resembling the canonical Oct-Sox motif. TOBF1 physically interacts with Panct1 and exhibits a cell-cycle-specific punctate localization in ESCs. At the chromatin level, this correlates with its recruitment to promoters of pluripotency genes. Strikingly, mutating an octamer-like motif in Panct1 RNA abrogates the strength of TOBF1 localization and recruitment to its targets. Taken together, our data reveal a tightly controlled spatial and temporal pattern of lncRNA-mediated gene regulation in a cell-cycle-dependent manner and suggest that lncRNAs might function as barcodes for identifying genomic addresses for maintaining cellular states.

KW - Journal Article

U2 - 10.1016/j.celrep.2017.11.045

DO - 10.1016/j.celrep.2017.11.045

M3 - Journal article

C2 - 29241531

VL - 21

SP - 3012

EP - 3021

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 11

ER -

ID: 186863631