LNA-antisense rivals siRNA for gene silencing

Research output: Contribution to journalJournal articlepeer-review

Locked nucleic acid (LNA) is a class of nucleic acid analogs possessing unprecedented binding affinity toward complementary DNA and RNA while obeying the Watson-Crick base-pairing rules. For efficient gene silencing in vitro and in vivo, fully modified or chimeric LNA oligonucleotides have been applied. LNA oligonucleotides are commercially available, can be transfected using standard techniques, are non-toxic, lead to increased target accessibility, can be designed to activate RNase H, and function in steric block approaches. LNA-Antisense, including gapmer LNA containing a central DNA or phosphorothioate-DNA segment flanked by LNA gaps, rivals siRNA as the technology of choice for target validation and therapeutic applications.
Original languageEnglish
JournalCurrent Opinion in Drug Discovery & Development
Volume7
Issue number2
Pages (from-to)188-94
Number of pages7
ISSN1367-6733
Publication statusPublished - Mar 2004
Externally publishedYes

    Research areas

  • Animals, Drug Design, Gene Silencing, Genetic Therapy, Humans, Oligonucleotides, Oligonucleotides, Antisense, RNA, Small Interfering, Ribonuclease H

ID: 59323974