Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy

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Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy. / Kaasgaard, Thomas; Mouritsen, Ole G.; Jorgensen, Kent.

In: FEBS Letters, Vol. 515, No. 1-3, 27.03.2002, p. 29-34.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kaasgaard, T, Mouritsen, OG & Jorgensen, K 2002, 'Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy', FEBS Letters, vol. 515, no. 1-3, pp. 29-34. https://doi.org/10.1016/S0014-5793(02)02391-8

APA

Kaasgaard, T., Mouritsen, O. G., & Jorgensen, K. (2002). Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy. FEBS Letters, 515(1-3), 29-34. https://doi.org/10.1016/S0014-5793(02)02391-8

Vancouver

Kaasgaard T, Mouritsen OG, Jorgensen K. Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy. FEBS Letters. 2002 Mar 27;515(1-3):29-34. https://doi.org/10.1016/S0014-5793(02)02391-8

Author

Kaasgaard, Thomas ; Mouritsen, Ole G. ; Jorgensen, Kent. / Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy. In: FEBS Letters. 2002 ; Vol. 515, No. 1-3. pp. 29-34.

Bibtex

@article{9790122eaed64b358b8f342e5cb76bdc,

title = "Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy",

abstract = "A novel experimental technique, based on atomic force microscopy (AFM), is proposed to visualize the lateral organization of membrane systems in the nanometer range. The technique involves the use of a ligand-receptor pair, biotin-avidin, which introduces a height variation on a solid-supported lipid bilayer membrane. This leads to a height amplification of the lateral membrane organization that is large enough to be clearly imaged by scanning AFM. The power of the technique is demonstrated for a binary dipalmitoylphosphocholine-diarachidoylphosphocholine lipid mixture which is shown to exhibit a distinct lateral lipid domain formation. The new and simple ligand-receptor-based AFM approach opens up new ways to investigate lipid membrane microstructure in the nanometer range as well as the lateral distribution of ligand-lipid and receptor-protein complexes in supported membrane systems.",

keywords = "Atomic force microscopy, Avidin, Biotin, Langmuir-Blodgett film, Lipid bilayer membrane, Lipid domain, Lipid membrane heterogeneity, Lipid mixture, Phosphatidylcholine",

author = "Thomas Kaasgaard and Mouritsen, {Ole G.} and Kent Jorgensen",

year = "2002",

month = mar,

day = "27",

doi = "10.1016/S0014-5793(02)02391-8",

language = "English",

volume = "515",

pages = "29--34",

journal = "F E B S Letters",

issn = "0014-5793",

publisher = "JohnWiley & Sons Ltd",

number = "1-3",

}

RIS

TY - JOUR

T1 - Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy

AU - Kaasgaard, Thomas

AU - Mouritsen, Ole G.

AU - Jorgensen, Kent

PY - 2002/3/27

Y1 - 2002/3/27

N2 - A novel experimental technique, based on atomic force microscopy (AFM), is proposed to visualize the lateral organization of membrane systems in the nanometer range. The technique involves the use of a ligand-receptor pair, biotin-avidin, which introduces a height variation on a solid-supported lipid bilayer membrane. This leads to a height amplification of the lateral membrane organization that is large enough to be clearly imaged by scanning AFM. The power of the technique is demonstrated for a binary dipalmitoylphosphocholine-diarachidoylphosphocholine lipid mixture which is shown to exhibit a distinct lateral lipid domain formation. The new and simple ligand-receptor-based AFM approach opens up new ways to investigate lipid membrane microstructure in the nanometer range as well as the lateral distribution of ligand-lipid and receptor-protein complexes in supported membrane systems.

AB - A novel experimental technique, based on atomic force microscopy (AFM), is proposed to visualize the lateral organization of membrane systems in the nanometer range. The technique involves the use of a ligand-receptor pair, biotin-avidin, which introduces a height variation on a solid-supported lipid bilayer membrane. This leads to a height amplification of the lateral membrane organization that is large enough to be clearly imaged by scanning AFM. The power of the technique is demonstrated for a binary dipalmitoylphosphocholine-diarachidoylphosphocholine lipid mixture which is shown to exhibit a distinct lateral lipid domain formation. The new and simple ligand-receptor-based AFM approach opens up new ways to investigate lipid membrane microstructure in the nanometer range as well as the lateral distribution of ligand-lipid and receptor-protein complexes in supported membrane systems.

KW - Atomic force microscopy

KW - Avidin

KW - Biotin

KW - Langmuir-Blodgett film

KW - Lipid bilayer membrane

KW - Lipid domain

KW - Lipid membrane heterogeneity

KW - Lipid mixture

KW - Phosphatidylcholine

UR - http://www.scopus.com/inward/record.url?scp=0037181461&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(02)02391-8

DO - 10.1016/S0014-5793(02)02391-8

M3 - Journal article

C2 - 11943189

AN - SCOPUS:0037181461

VL - 515

SP - 29

EP - 34

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 1-3

ER -

ID: 230986874