Light-driven oxidation of polysaccharides by photosynthetic pigments and a metalloenzyme

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Light-driven oxidation of polysaccharides by photosynthetic pigments and a metalloenzyme. / Cannella, David; Möllers, K. Benedikt; Frigaard, Niels-Ulrik; Jensen, Poul Erik; Bjerrum, Morten Jannik; Johansen, Katja Salomon; Felby, Claus.

In: Nature Communications, Vol. 7, 11134, 2016.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Cannella, D, Möllers, KB, Frigaard, N-U, Jensen, PE, Bjerrum, MJ, Johansen, KS & Felby, C 2016, 'Light-driven oxidation of polysaccharides by photosynthetic pigments and a metalloenzyme', Nature Communications, vol. 7, 11134. https://doi.org/10.1038/ncomms11134

APA

Cannella, D., Möllers, K. B., Frigaard, N-U., Jensen, P. E., Bjerrum, M. J., Johansen, K. S., & Felby, C. (2016). Light-driven oxidation of polysaccharides by photosynthetic pigments and a metalloenzyme. Nature Communications, 7, [11134]. https://doi.org/10.1038/ncomms11134

Vancouver

Cannella D, Möllers KB, Frigaard N-U, Jensen PE, Bjerrum MJ, Johansen KS et al. Light-driven oxidation of polysaccharides by photosynthetic pigments and a metalloenzyme. Nature Communications. 2016;7. 11134. https://doi.org/10.1038/ncomms11134

Author

Cannella, David ; Möllers, K. Benedikt ; Frigaard, Niels-Ulrik ; Jensen, Poul Erik ; Bjerrum, Morten Jannik ; Johansen, Katja Salomon ; Felby, Claus. / Light-driven oxidation of polysaccharides by photosynthetic pigments and a metalloenzyme. In: Nature Communications. 2016 ; Vol. 7.

Bibtex

@article{44c9e9ad4d9d4f8ab567a1cc90af8b8f,
title = "Light-driven oxidation of polysaccharides by photosynthetic pigments and a metalloenzyme",
abstract = "Oxidative processes are essential for the degradation of plant biomass. A class of powerful and widely distributed oxidative enzymes, the lytic polysaccharide monooxygenases (LPMOs), oxidize the most recalcitrant polysaccharides and require extracellular electron donors. Here we investigated the effect of using excited photosynthetic pigments as electron donors. LPMOs combined with pigments and reducing agents were exposed to light, which resulted in a never before seen 100-fold increase in catalytic activity. In addition, LPMO substrate specificity was broadened to include both cellulose and hemicellulose. LPMO enzymes and pigment derivatives common in the environment of plant-degrading organisms thus form a highly reactive and stable light-driven system increasing the turnover rate and versatility of LPMOs. This light-driven system may find applications in biotechnology and chemical processing.",
author = "David Cannella and M{\"o}llers, {K. Benedikt} and Niels-Ulrik Frigaard and Jensen, {Poul Erik} and Bjerrum, {Morten Jannik} and Johansen, {Katja Salomon} and Claus Felby",
year = "2016",
doi = "10.1038/ncomms11134",
language = "English",
volume = "7",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Light-driven oxidation of polysaccharides by photosynthetic pigments and a metalloenzyme

AU - Cannella, David

AU - Möllers, K. Benedikt

AU - Frigaard, Niels-Ulrik

AU - Jensen, Poul Erik

AU - Bjerrum, Morten Jannik

AU - Johansen, Katja Salomon

AU - Felby, Claus

PY - 2016

Y1 - 2016

N2 - Oxidative processes are essential for the degradation of plant biomass. A class of powerful and widely distributed oxidative enzymes, the lytic polysaccharide monooxygenases (LPMOs), oxidize the most recalcitrant polysaccharides and require extracellular electron donors. Here we investigated the effect of using excited photosynthetic pigments as electron donors. LPMOs combined with pigments and reducing agents were exposed to light, which resulted in a never before seen 100-fold increase in catalytic activity. In addition, LPMO substrate specificity was broadened to include both cellulose and hemicellulose. LPMO enzymes and pigment derivatives common in the environment of plant-degrading organisms thus form a highly reactive and stable light-driven system increasing the turnover rate and versatility of LPMOs. This light-driven system may find applications in biotechnology and chemical processing.

AB - Oxidative processes are essential for the degradation of plant biomass. A class of powerful and widely distributed oxidative enzymes, the lytic polysaccharide monooxygenases (LPMOs), oxidize the most recalcitrant polysaccharides and require extracellular electron donors. Here we investigated the effect of using excited photosynthetic pigments as electron donors. LPMOs combined with pigments and reducing agents were exposed to light, which resulted in a never before seen 100-fold increase in catalytic activity. In addition, LPMO substrate specificity was broadened to include both cellulose and hemicellulose. LPMO enzymes and pigment derivatives common in the environment of plant-degrading organisms thus form a highly reactive and stable light-driven system increasing the turnover rate and versatility of LPMOs. This light-driven system may find applications in biotechnology and chemical processing.

U2 - 10.1038/ncomms11134

DO - 10.1038/ncomms11134

M3 - Journal article

C2 - 27041218

VL - 7

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 11134

ER -

ID: 160191479