Leucine-based receptor sorting motifs are dependent on the spacing relative to the plasma membrane

Research output: Contribution to journalJournal articlepeer-review

Standard

Leucine-based receptor sorting motifs are dependent on the spacing relative to the plasma membrane. / Geisler, C; Dietrich, J; Nielsen, B L; Kastrup, J; Lauritsen, Jens Peter Holst; Odum, Niels; Christensen, M D.

In: Journal of Biological Chemistry, Vol. 273, No. 33, 1998, p. 21316-23.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Geisler, C, Dietrich, J, Nielsen, BL, Kastrup, J, Lauritsen, JPH, Odum, N & Christensen, MD 1998, 'Leucine-based receptor sorting motifs are dependent on the spacing relative to the plasma membrane', Journal of Biological Chemistry, vol. 273, no. 33, pp. 21316-23.

APA

Geisler, C., Dietrich, J., Nielsen, B. L., Kastrup, J., Lauritsen, J. P. H., Odum, N., & Christensen, M. D. (1998). Leucine-based receptor sorting motifs are dependent on the spacing relative to the plasma membrane. Journal of Biological Chemistry, 273(33), 21316-23.

Vancouver

Geisler C, Dietrich J, Nielsen BL, Kastrup J, Lauritsen JPH, Odum N et al. Leucine-based receptor sorting motifs are dependent on the spacing relative to the plasma membrane. Journal of Biological Chemistry. 1998;273(33):21316-23.

Author

Geisler, C ; Dietrich, J ; Nielsen, B L ; Kastrup, J ; Lauritsen, Jens Peter Holst ; Odum, Niels ; Christensen, M D. / Leucine-based receptor sorting motifs are dependent on the spacing relative to the plasma membrane. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 33. pp. 21316-23.

Bibtex

@article{06ba7130b0a411ddb538000ea68e967b,
title = "Leucine-based receptor sorting motifs are dependent on the spacing relative to the plasma membrane",
abstract = "Many integral membrane proteins contain leucine-based motifs within their cytoplasmic domains that mediate internalization and intracellular sorting. Two types of leucine-based motifs have been identified. One type is dependent on phosphorylation, whereas the other type, which includes an acidic amino acid, is constitutively active. In this study, we have investigated how the spacing relative to the plasma membrane affects the function of both types of leucine-based motifs. For phosphorylation-dependent leucine-based motifs, a minimal spacing of 7 residues between the plasma membrane and the phospho-acceptor was required for phosphorylation and thereby activation of the motifs. For constitutively active leucine-based motifs, a minimal spacing of 6 residues between the plasma membrane and the acidic residue was required for optimal activity of the motifs. In addition, we found that the acidic residue of leucine-based motifs must be located amino-terminal to the dileucine sequence for proper function of the motifs and that residues surrounding the motifs affect the activity of the motifs. Thus, our observations suggest that the position, the exact sequence, and surrounding residues are major determinants of the function of leucine-based receptor sorting motifs.",
author = "C Geisler and J Dietrich and Nielsen, {B L} and J Kastrup and Lauritsen, {Jens Peter Holst} and Niels Odum and Christensen, {M D}",
note = "Keywords: Amino Acid Sequence; Base Sequence; Cell Membrane; DNA Primers; Endocytosis; Humans; Jurkat Cells; Leucine; Molecular Sequence Data; Phosphorylation; Receptors, Antigen, T-Cell; Sequence Homology, Amino Acid",
year = "1998",
language = "English",
volume = "273",
pages = "21316--23",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "33",

}

RIS

TY - JOUR

T1 - Leucine-based receptor sorting motifs are dependent on the spacing relative to the plasma membrane

AU - Geisler, C

AU - Dietrich, J

AU - Nielsen, B L

AU - Kastrup, J

AU - Lauritsen, Jens Peter Holst

AU - Odum, Niels

AU - Christensen, M D

N1 - Keywords: Amino Acid Sequence; Base Sequence; Cell Membrane; DNA Primers; Endocytosis; Humans; Jurkat Cells; Leucine; Molecular Sequence Data; Phosphorylation; Receptors, Antigen, T-Cell; Sequence Homology, Amino Acid

PY - 1998

Y1 - 1998

N2 - Many integral membrane proteins contain leucine-based motifs within their cytoplasmic domains that mediate internalization and intracellular sorting. Two types of leucine-based motifs have been identified. One type is dependent on phosphorylation, whereas the other type, which includes an acidic amino acid, is constitutively active. In this study, we have investigated how the spacing relative to the plasma membrane affects the function of both types of leucine-based motifs. For phosphorylation-dependent leucine-based motifs, a minimal spacing of 7 residues between the plasma membrane and the phospho-acceptor was required for phosphorylation and thereby activation of the motifs. For constitutively active leucine-based motifs, a minimal spacing of 6 residues between the plasma membrane and the acidic residue was required for optimal activity of the motifs. In addition, we found that the acidic residue of leucine-based motifs must be located amino-terminal to the dileucine sequence for proper function of the motifs and that residues surrounding the motifs affect the activity of the motifs. Thus, our observations suggest that the position, the exact sequence, and surrounding residues are major determinants of the function of leucine-based receptor sorting motifs.

AB - Many integral membrane proteins contain leucine-based motifs within their cytoplasmic domains that mediate internalization and intracellular sorting. Two types of leucine-based motifs have been identified. One type is dependent on phosphorylation, whereas the other type, which includes an acidic amino acid, is constitutively active. In this study, we have investigated how the spacing relative to the plasma membrane affects the function of both types of leucine-based motifs. For phosphorylation-dependent leucine-based motifs, a minimal spacing of 7 residues between the plasma membrane and the phospho-acceptor was required for phosphorylation and thereby activation of the motifs. For constitutively active leucine-based motifs, a minimal spacing of 6 residues between the plasma membrane and the acidic residue was required for optimal activity of the motifs. In addition, we found that the acidic residue of leucine-based motifs must be located amino-terminal to the dileucine sequence for proper function of the motifs and that residues surrounding the motifs affect the activity of the motifs. Thus, our observations suggest that the position, the exact sequence, and surrounding residues are major determinants of the function of leucine-based receptor sorting motifs.

M3 - Journal article

C2 - 9694892

VL - 273

SP - 21316

EP - 21323

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 33

ER -

ID: 8545403