Large-Scale Phosphoproteomics Reveals Shp-2 Phosphatase-Dependent Regulators of Pdgf Receptor Signaling
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Large-Scale Phosphoproteomics Reveals Shp-2 Phosphatase-Dependent Regulators of Pdgf Receptor Signaling. / Batth, Tanveer S.; Papetti, Moreno; Pfeiffer, Anamarija; Tollenaere, Maxim A.X.; Francavilla, Chiara; Olsen, Jesper V.
In: Cell Reports, Vol. 22, No. 10, 2018, p. 2784-2796.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Large-Scale Phosphoproteomics Reveals Shp-2 Phosphatase-Dependent Regulators of Pdgf Receptor Signaling
AU - Batth, Tanveer S.
AU - Papetti, Moreno
AU - Pfeiffer, Anamarija
AU - Tollenaere, Maxim A.X.
AU - Francavilla, Chiara
AU - Olsen, Jesper V.
N1 - Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2018
Y1 - 2018
N2 - Despite its low cellular abundance, phosphotyrosine (pTyr) regulates numerous cell signaling pathways in health and disease. We applied comprehensive phosphoproteomics to unravel differential regulators of receptor tyrosine kinase (RTK)-initiated signaling networks upon activation by Pdgf-ββ, Fgf-2, or Igf-1 and identified more than 40,000 phosphorylation sites, including many phosphotyrosine sites without additional enrichment. The analysis revealed RTK-specific regulation of hundreds of pTyr sites on key signaling molecules. We found the tyrosine phosphatase Shp-2 to be the master regulator of Pdgfr pTyr signaling. Application of a recently introduced allosteric Shp-2 inhibitor revealed global regulation of the Pdgf-dependent tyrosine phosphoproteome, which significantly impaired cell migration. In addition, we present a list of hundreds of Shp-2-dependent targets and putative substrates, including Rasa1 and Cortactin with increased pTyr and Gab1 and Erk1/2 with decreased pTyr. Our study demonstrates that large-scale quantitative phosphoproteomics can precisely dissect tightly regulated kinase-phosphatase signaling networks.
AB - Despite its low cellular abundance, phosphotyrosine (pTyr) regulates numerous cell signaling pathways in health and disease. We applied comprehensive phosphoproteomics to unravel differential regulators of receptor tyrosine kinase (RTK)-initiated signaling networks upon activation by Pdgf-ββ, Fgf-2, or Igf-1 and identified more than 40,000 phosphorylation sites, including many phosphotyrosine sites without additional enrichment. The analysis revealed RTK-specific regulation of hundreds of pTyr sites on key signaling molecules. We found the tyrosine phosphatase Shp-2 to be the master regulator of Pdgfr pTyr signaling. Application of a recently introduced allosteric Shp-2 inhibitor revealed global regulation of the Pdgf-dependent tyrosine phosphoproteome, which significantly impaired cell migration. In addition, we present a list of hundreds of Shp-2-dependent targets and putative substrates, including Rasa1 and Cortactin with increased pTyr and Gab1 and Erk1/2 with decreased pTyr. Our study demonstrates that large-scale quantitative phosphoproteomics can precisely dissect tightly regulated kinase-phosphatase signaling networks.
KW - label-free quantitation
KW - mass spectrometry
KW - orbitrap
KW - PDGF
KW - phosphoproteomics
KW - Q exactive
KW - Shp-2
KW - SHP099
KW - TiO2
KW - tyrosine phosphorylation
U2 - 10.1016/j.celrep.2018.02.038
DO - 10.1016/j.celrep.2018.02.038
M3 - Journal article
C2 - 29514104
VL - 22
SP - 2784
EP - 2796
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 10
ER -
ID: 192400927