Keratinocytes negatively regulate the N-cadherin levels of melanoma cells via contact-mediated calcium regulation

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Keratinocytes negatively regulate the N-cadherin levels of melanoma cells via contact-mediated calcium regulation. / Chung, Heesung; Jung, Hyejung; Jho, Eek-Hoon; Multhaupt, Hinke A. B.; Couchman, John R; Oh, Eok-Soo.

In: Biochemical and Biophysical Research Communications, Vol. 503, No. 2, 2018, p. 615-620.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Chung, H, Jung, H, Jho, E-H, Multhaupt, HAB, Couchman, JR & Oh, E-S 2018, 'Keratinocytes negatively regulate the N-cadherin levels of melanoma cells via contact-mediated calcium regulation', Biochemical and Biophysical Research Communications, vol. 503, no. 2, pp. 615-620. https://doi.org/10.1016/j.bbrc.2018.06.050

APA

Chung, H., Jung, H., Jho, E-H., Multhaupt, H. A. B., Couchman, J. R., & Oh, E-S. (2018). Keratinocytes negatively regulate the N-cadherin levels of melanoma cells via contact-mediated calcium regulation. Biochemical and Biophysical Research Communications, 503(2), 615-620. https://doi.org/10.1016/j.bbrc.2018.06.050

Vancouver

Chung H, Jung H, Jho E-H, Multhaupt HAB, Couchman JR, Oh E-S. Keratinocytes negatively regulate the N-cadherin levels of melanoma cells via contact-mediated calcium regulation. Biochemical and Biophysical Research Communications. 2018;503(2):615-620. https://doi.org/10.1016/j.bbrc.2018.06.050

Author

Chung, Heesung ; Jung, Hyejung ; Jho, Eek-Hoon ; Multhaupt, Hinke A. B. ; Couchman, John R ; Oh, Eok-Soo. / Keratinocytes negatively regulate the N-cadherin levels of melanoma cells via contact-mediated calcium regulation. In: Biochemical and Biophysical Research Communications. 2018 ; Vol. 503, No. 2. pp. 615-620.

Bibtex

@article{0732789adf194fe2add88c83526fcabc,
title = "Keratinocytes negatively regulate the N-cadherin levels of melanoma cells via contact-mediated calcium regulation",
abstract = "In human skin, melanocytes and their neighboring keratinocytes have a close functional interrelationship. Keratinocytes, which represent the prevalent cell type of human skin, regulate melanocytes through various mechanisms. Here, we use a keratinocyte and melanoma co-culture system to show for the first time that keratinocytes regulate the cell surface expression of N-cadherin through cell-cell contact. Compared to mono-cultured human melanoma A375 cells, which expressed high levels of N-cadherin, those co-cultured with the HaCaT human keratinocyte cell line showed reduced levels of N-cadherin. This reduction was most evident in areas of A375 cells that underwent cell-cell contact with the HaCaT cells, whereas HaCaT cell-derived extracellular matrix and conditioned medium both failed to reduce N-cadherin levels. The intracellular level of calcium in co-cultured A375 cells was lower than that in mono-cultured A375 cells, and treatment with a cell-permeant calcium chelator (BAPTA) reduced the N-cadherin level of mono-cultured A375 cells. Furthermore, co-culture with HaCaT cells reduced the expression levels of transient receptor potential cation channel (TRPC) 1, -3 and -6 in A375 cells, and siRNA-mediated multi-depletion of TRPC1, -3 and -6 reduced the N-cadherin level in these cells. Taken together, these data suggest that keratinocytes negatively regulate the N-cadherin levels of melanoma cells via cell-to-cell contact-mediated calcium regulation.",
author = "Heesung Chung and Hyejung Jung and Eek-Hoon Jho and Multhaupt, {Hinke A. B.} and Couchman, {John R} and Eok-Soo Oh",
note = "Copyright {\circledC} 2018 Elsevier Inc. All rights reserved.",
year = "2018",
doi = "10.1016/j.bbrc.2018.06.050",
language = "English",
volume = "503",
pages = "615--620",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Keratinocytes negatively regulate the N-cadherin levels of melanoma cells via contact-mediated calcium regulation

AU - Chung, Heesung

AU - Jung, Hyejung

AU - Jho, Eek-Hoon

AU - Multhaupt, Hinke A. B.

AU - Couchman, John R

AU - Oh, Eok-Soo

N1 - Copyright © 2018 Elsevier Inc. All rights reserved.

PY - 2018

Y1 - 2018

N2 - In human skin, melanocytes and their neighboring keratinocytes have a close functional interrelationship. Keratinocytes, which represent the prevalent cell type of human skin, regulate melanocytes through various mechanisms. Here, we use a keratinocyte and melanoma co-culture system to show for the first time that keratinocytes regulate the cell surface expression of N-cadherin through cell-cell contact. Compared to mono-cultured human melanoma A375 cells, which expressed high levels of N-cadherin, those co-cultured with the HaCaT human keratinocyte cell line showed reduced levels of N-cadherin. This reduction was most evident in areas of A375 cells that underwent cell-cell contact with the HaCaT cells, whereas HaCaT cell-derived extracellular matrix and conditioned medium both failed to reduce N-cadherin levels. The intracellular level of calcium in co-cultured A375 cells was lower than that in mono-cultured A375 cells, and treatment with a cell-permeant calcium chelator (BAPTA) reduced the N-cadherin level of mono-cultured A375 cells. Furthermore, co-culture with HaCaT cells reduced the expression levels of transient receptor potential cation channel (TRPC) 1, -3 and -6 in A375 cells, and siRNA-mediated multi-depletion of TRPC1, -3 and -6 reduced the N-cadherin level in these cells. Taken together, these data suggest that keratinocytes negatively regulate the N-cadherin levels of melanoma cells via cell-to-cell contact-mediated calcium regulation.

AB - In human skin, melanocytes and their neighboring keratinocytes have a close functional interrelationship. Keratinocytes, which represent the prevalent cell type of human skin, regulate melanocytes through various mechanisms. Here, we use a keratinocyte and melanoma co-culture system to show for the first time that keratinocytes regulate the cell surface expression of N-cadherin through cell-cell contact. Compared to mono-cultured human melanoma A375 cells, which expressed high levels of N-cadherin, those co-cultured with the HaCaT human keratinocyte cell line showed reduced levels of N-cadherin. This reduction was most evident in areas of A375 cells that underwent cell-cell contact with the HaCaT cells, whereas HaCaT cell-derived extracellular matrix and conditioned medium both failed to reduce N-cadherin levels. The intracellular level of calcium in co-cultured A375 cells was lower than that in mono-cultured A375 cells, and treatment with a cell-permeant calcium chelator (BAPTA) reduced the N-cadherin level of mono-cultured A375 cells. Furthermore, co-culture with HaCaT cells reduced the expression levels of transient receptor potential cation channel (TRPC) 1, -3 and -6 in A375 cells, and siRNA-mediated multi-depletion of TRPC1, -3 and -6 reduced the N-cadherin level in these cells. Taken together, these data suggest that keratinocytes negatively regulate the N-cadherin levels of melanoma cells via cell-to-cell contact-mediated calcium regulation.

U2 - 10.1016/j.bbrc.2018.06.050

DO - 10.1016/j.bbrc.2018.06.050

M3 - Journal article

C2 - 29902459

VL - 503

SP - 615

EP - 620

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

ID: 203671472