Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

  • H. Stålbrand
  • J. Lundqvist
  • A. Andersson
  • Hägglund, Per Mårten
  • L. Anderson
  • F. Tjerneld
  • A. Jacobs
  • A. Teleman
  • O. Dahlman
  • M. Palm
  • G. Zacchi

Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190° C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using 1H-NMR. The enzymatic hydrolysis of AcGGM was studied using β-mannanase and α-galactosidase.

Original languageEnglish
Title of host publicationHenicelluloses : Science and Technology
EditorsPaul Gatenholm, Maija Tenkanen
Number of pages13
Publication date1 Dec 2004
ISBN (Print)9780841238428
Publication statusPublished - 1 Dec 2004
SeriesA C S Symposium Series

ID: 240161930