Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Standard

Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies). / Stålbrand, H.; Lundqvist, J.; Andersson, A.; Hägglund, P.; Anderson, L.; Tjerneld, F.; Jacobs, A.; Teleman, A.; Dahlman, O.; Palm, M.; Zacchi, G.

Henicelluloses: Science and Technology. ed. / Paul Gatenholm; Maija Tenkanen. 2004. p. 66-78 (A C S Symposium Series, Vol. 864).

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Harvard

Stålbrand, H, Lundqvist, J, Andersson, A, Hägglund, P, Anderson, L, Tjerneld, F, Jacobs, A, Teleman, A, Dahlman, O, Palm, M & Zacchi, G 2004, Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies). in P Gatenholm & M Tenkanen (eds), Henicelluloses: Science and Technology. A C S Symposium Series, vol. 864, pp. 66-78.

APA

Stålbrand, H., Lundqvist, J., Andersson, A., Hägglund, P., Anderson, L., Tjerneld, F., Jacobs, A., Teleman, A., Dahlman, O., Palm, M., & Zacchi, G. (2004). Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies). In P. Gatenholm, & M. Tenkanen (Eds.), Henicelluloses: Science and Technology (pp. 66-78). A C S Symposium Series Vol. 864

Vancouver

Stålbrand H, Lundqvist J, Andersson A, Hägglund P, Anderson L, Tjerneld F et al. Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies). In Gatenholm P, Tenkanen M, editors, Henicelluloses: Science and Technology. 2004. p. 66-78. (A C S Symposium Series, Vol. 864).

Author

Stålbrand, H. ; Lundqvist, J. ; Andersson, A. ; Hägglund, P. ; Anderson, L. ; Tjerneld, F. ; Jacobs, A. ; Teleman, A. ; Dahlman, O. ; Palm, M. ; Zacchi, G. / Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies). Henicelluloses: Science and Technology. editor / Paul Gatenholm ; Maija Tenkanen. 2004. pp. 66-78 (A C S Symposium Series, Vol. 864).

Bibtex

@inproceedings{d3b3c46e5e0647818dc6959bb3decf2f,
title = "Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)",
abstract = "Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190° C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using 1H-NMR. The enzymatic hydrolysis of AcGGM was studied using β-mannanase and α-galactosidase.",
author = "H. St{\aa}lbrand and J. Lundqvist and A. Andersson and P. H{\"a}gglund and L. Anderson and F. Tjerneld and A. Jacobs and A. Teleman and O. Dahlman and M. Palm and G. Zacchi",
year = "2004",
month = dec,
day = "1",
language = "English",
isbn = "9780841238428",
series = "A C S Symposium Series",
publisher = "ACS Publications",
pages = "66--78",
editor = "Paul Gatenholm and Maija Tenkanen",
booktitle = "Henicelluloses",

}

RIS

TY - GEN

T1 - Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)

AU - Stålbrand, H.

AU - Lundqvist, J.

AU - Andersson, A.

AU - Hägglund, P.

AU - Anderson, L.

AU - Tjerneld, F.

AU - Jacobs, A.

AU - Teleman, A.

AU - Dahlman, O.

AU - Palm, M.

AU - Zacchi, G.

PY - 2004/12/1

Y1 - 2004/12/1

N2 - Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190° C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using 1H-NMR. The enzymatic hydrolysis of AcGGM was studied using β-mannanase and α-galactosidase.

AB - Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190° C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using 1H-NMR. The enzymatic hydrolysis of AcGGM was studied using β-mannanase and α-galactosidase.

UR - http://www.scopus.com/inward/record.url?scp=38849099768&partnerID=8YFLogxK

M3 - Article in proceedings

AN - SCOPUS:38849099768

SN - 9780841238428

T3 - A C S Symposium Series

SP - 66

EP - 78

BT - Henicelluloses

A2 - Gatenholm, Paul

A2 - Tenkanen, Maija

ER -

ID: 240161930