Isolation and characterisation of novel phages infecting Lactobacillus plantarum and proposal of a new genus, "Silenusvirus"
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Isolation and characterisation of novel phages infecting Lactobacillus plantarum and proposal of a new genus, "Silenusvirus". / Kyrkou, Ifigeneia; Carstens, Alexander Byth; Ellegaard-Jensen, Lea; Kot, Witold; Zervas, Athanasios; Djurhuus, Amaru Miranda; Neve, Horst; Franz, Charles M. A. P.; Hansen, Martin; Hansen, Lars Hestbjerg.
In: Scientific Reports, Vol. 10, No. 1, 8763, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Isolation and characterisation of novel phages infecting Lactobacillus plantarum and proposal of a new genus, "Silenusvirus"
AU - Kyrkou, Ifigeneia
AU - Carstens, Alexander Byth
AU - Ellegaard-Jensen, Lea
AU - Kot, Witold
AU - Zervas, Athanasios
AU - Djurhuus, Amaru Miranda
AU - Neve, Horst
AU - Franz, Charles M. A. P.
AU - Hansen, Martin
AU - Hansen, Lars Hestbjerg
PY - 2020
Y1 - 2020
N2 - Bacteria of Lactobacillus sp. are very useful to humans. However, the biology and genomic diversity of their (bacterio)phage enemies remains understudied. Knowledge on Lactobacillus phage diversity should broaden to develop efficient phage control strategies. To this end, organic waste samples were screened for phages against two wine-related Lactobacillus plantarum strains. Isolates were shotgun sequenced and compared against the phage database and each other by phylogenetics and comparative genomics. The new isolates had only three distant relatives from the database, but displayed a high overall degree of genomic similarity amongst them. The latter allowed for the use of one isolate as a representative to conduct transmission electron microscopy and structural protein sequencing, and to study phage adsorption and growth kinetics. The microscopy and proteomics tests confirmed the observed diversity of the new isolates and supported their classification to the family Siphoviridae and the proposal of the new phage genus "Silenusvirus".
AB - Bacteria of Lactobacillus sp. are very useful to humans. However, the biology and genomic diversity of their (bacterio)phage enemies remains understudied. Knowledge on Lactobacillus phage diversity should broaden to develop efficient phage control strategies. To this end, organic waste samples were screened for phages against two wine-related Lactobacillus plantarum strains. Isolates were shotgun sequenced and compared against the phage database and each other by phylogenetics and comparative genomics. The new isolates had only three distant relatives from the database, but displayed a high overall degree of genomic similarity amongst them. The latter allowed for the use of one isolate as a representative to conduct transmission electron microscopy and structural protein sequencing, and to study phage adsorption and growth kinetics. The microscopy and proteomics tests confirmed the observed diversity of the new isolates and supported their classification to the family Siphoviridae and the proposal of the new phage genus "Silenusvirus".
KW - PROTEIN
KW - BACTERIOPHAGES
KW - CLASSIFICATION
KW - FRAMESHIFT
KW - SEQUENCE
U2 - 10.1038/s41598-020-65366-6
DO - 10.1038/s41598-020-65366-6
M3 - Journal article
C2 - 32472049
VL - 10
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 8763
ER -
ID: 249486899