Investigation of factors affecting the stability of lysozyme spray dried from ethanol-water solutions

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Standard

Investigation of factors affecting the stability of lysozyme spray dried from ethanol-water solutions. / Ji, Shuying; Thulstrup, Peter Waaben; Mu, Huiling; Hansen, Steen Honoré; van de Weert, Marco; Rantanen, Jukka; Yang, Mingshi.

In: International Journal of Pharmaceutics, Vol. 534, No. 1-2, 2017, p. 263-271.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ji, S, Thulstrup, PW, Mu, H, Hansen, SH, van de Weert, M, Rantanen, J & Yang, M 2017, 'Investigation of factors affecting the stability of lysozyme spray dried from ethanol-water solutions', International Journal of Pharmaceutics, vol. 534, no. 1-2, pp. 263-271. https://doi.org/10.1016/j.ijpharm.2017.10.021

APA

Ji, S., Thulstrup, P. W., Mu, H., Hansen, S. H., van de Weert, M., Rantanen, J., & Yang, M. (2017). Investigation of factors affecting the stability of lysozyme spray dried from ethanol-water solutions. International Journal of Pharmaceutics, 534(1-2), 263-271. https://doi.org/10.1016/j.ijpharm.2017.10.021

Vancouver

Ji S, Thulstrup PW, Mu H, Hansen SH, van de Weert M, Rantanen J et al. Investigation of factors affecting the stability of lysozyme spray dried from ethanol-water solutions. International Journal of Pharmaceutics. 2017;534(1-2):263-271. https://doi.org/10.1016/j.ijpharm.2017.10.021

Author

Ji, Shuying ; Thulstrup, Peter Waaben ; Mu, Huiling ; Hansen, Steen Honoré ; van de Weert, Marco ; Rantanen, Jukka ; Yang, Mingshi. / Investigation of factors affecting the stability of lysozyme spray dried from ethanol-water solutions. In: International Journal of Pharmaceutics. 2017 ; Vol. 534, No. 1-2. pp. 263-271.

Bibtex

@article{1b8888d8568740ac81f8a736307bcccd,

title = "Investigation of factors affecting the stability of lysozyme spray dried from ethanol-water solutions",

abstract = "Formulation composition and processing conditions can be adjusted to enhance the structural integrity as well as the bioactivity of proteins in the spray drying process. In this study, lysozyme was chosen as a model pharmaceutical protein to study these aspects when spray drying from water-ethanol mixtures. The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated. The results showed that the bioactivities of spray dried lysozyme with these additives were about 5-10% higher than that without additives. The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying. Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process. This suggests that the bioactivity of lysozyme could be preserved adequately by optimization of both the formulation composition and process conditions even when spray drying from a water-ethanol mixture.",

keywords = "Journal Article",

author = "Shuying Ji and Thulstrup, {Peter Waaben} and Huiling Mu and Hansen, {Steen Honor{\'e}} and {van de Weert}, Marco and Jukka Rantanen and Mingshi Yang",

year = "2017",

doi = "10.1016/j.ijpharm.2017.10.021",

language = "English",

volume = "534",

pages = "263--271",

journal = "International Journal of Pharmaceutics",

issn = "0378-5173",

publisher = "Elsevier",

number = "1-2",

}

RIS

TY - JOUR

T1 - Investigation of factors affecting the stability of lysozyme spray dried from ethanol-water solutions

AU - Ji, Shuying

AU - Thulstrup, Peter Waaben

AU - Mu, Huiling

AU - Hansen, Steen Honoré

AU - van de Weert, Marco

AU - Rantanen, Jukka

AU - Yang, Mingshi

PY - 2017

Y1 - 2017

N2 - Formulation composition and processing conditions can be adjusted to enhance the structural integrity as well as the bioactivity of proteins in the spray drying process. In this study, lysozyme was chosen as a model pharmaceutical protein to study these aspects when spray drying from water-ethanol mixtures. The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated. The results showed that the bioactivities of spray dried lysozyme with these additives were about 5-10% higher than that without additives. The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying. Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process. This suggests that the bioactivity of lysozyme could be preserved adequately by optimization of both the formulation composition and process conditions even when spray drying from a water-ethanol mixture.

AB - Formulation composition and processing conditions can be adjusted to enhance the structural integrity as well as the bioactivity of proteins in the spray drying process. In this study, lysozyme was chosen as a model pharmaceutical protein to study these aspects when spray drying from water-ethanol mixtures. The effect of formulation additives (trehalose, Tween 20 and phosphate-buffered saline) and processing conditions (inlet temperature and storage time of lysozyme in the feed solution before the spray drying process) on the protein bioactivity was investigated. The results showed that the bioactivities of spray dried lysozyme with these additives were about 5-10% higher than that without additives. The bioactivity of the spray dried lysozyme was found to increase with a decrease in the inlet temperature from 130°C to 80°C, with similar findings when shortening the storage time of the feed solutions prior to spray drying. Fourier Transform Infrared (FTIR) and Circular Dichroism (CD) results showed that the native structures of lysozyme were largely restored upon reconstitution of the spray dried powder in water after the spray drying process. This suggests that the bioactivity of lysozyme could be preserved adequately by optimization of both the formulation composition and process conditions even when spray drying from a water-ethanol mixture.

KW - Journal Article

U2 - 10.1016/j.ijpharm.2017.10.021

DO - 10.1016/j.ijpharm.2017.10.021

M3 - Journal article

C2 - 29031849

VL - 534

SP - 263

EP - 271

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 1-2

ER -

ID: 185402969