Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. / Balasubramaniam, Lakshmi; Doostmohammadi, Amin; Saw, Thuan Beng; Narayana, Gautham Hari Narayana Sankara; Mueller, Romain; Dang, Tien; Thomas, Minnah; Gupta, Shafali; Sonam, Surabhi; Yap, Alpha S.; Toyama, Yusuke; Mege, Rene-Marc; Yeomans, Julia M.; Ladoux, Benoit.

In: Nature Materials, Vol. 20, No. 8, 18.02.2021, p. 1156-1166.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Balasubramaniam, L, Doostmohammadi, A, Saw, TB, Narayana, GHNS, Mueller, R, Dang, T, Thomas, M, Gupta, S, Sonam, S, Yap, AS, Toyama, Y, Mege, R-M, Yeomans, JM & Ladoux, B 2021, 'Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers', Nature Materials, vol. 20, no. 8, pp. 1156-1166. https://doi.org/10.1038/s41563-021-00919-2

APA

Balasubramaniam, L., Doostmohammadi, A., Saw, T. B., Narayana, G. H. N. S., Mueller, R., Dang, T., Thomas, M., Gupta, S., Sonam, S., Yap, A. S., Toyama, Y., Mege, R-M., Yeomans, J. M., & Ladoux, B. (2021). Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. Nature Materials, 20(8), 1156-1166. https://doi.org/10.1038/s41563-021-00919-2

Vancouver

Balasubramaniam L, Doostmohammadi A, Saw TB, Narayana GHNS, Mueller R, Dang T et al. Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. Nature Materials. 2021 Feb 18;20(8):1156-1166. https://doi.org/10.1038/s41563-021-00919-2

Author

Balasubramaniam, Lakshmi ; Doostmohammadi, Amin ; Saw, Thuan Beng ; Narayana, Gautham Hari Narayana Sankara ; Mueller, Romain ; Dang, Tien ; Thomas, Minnah ; Gupta, Shafali ; Sonam, Surabhi ; Yap, Alpha S. ; Toyama, Yusuke ; Mege, Rene-Marc ; Yeomans, Julia M. ; Ladoux, Benoit. / Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. In: Nature Materials. 2021 ; Vol. 20, No. 8. pp. 1156-1166.

Bibtex

@article{b7e95959c73544e897f9127db65ab570,
title = "Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers",
abstract = "Actomyosin machinery endows cells with contractility at a single-cell level. However, within a monolayer, cells can be contractile or extensile based on the direction of pushing or pulling forces exerted by their neighbours or on the substrate. It has been shown that a monolayer of fibroblasts behaves as a contractile system while epithelial or neural progentior monolayers behave as an extensile system. Through a combination of cell culture experiments and in silico modelling, we reveal the mechanism behind this switch in extensile to contractile as the weakening of intercellular contacts. This switch promotes the build-up of tension at the cell-substrate interface through an increase in actin stress fibres and traction forces. This is accompanied by mechanotransductive changes in vinculin and YAP activation. We further show that contractile and extensile differences in cell activity sort cells in mixtures, uncovering a generic mechanism for pattern formation during cell competition, and morphogenesis.It is now revealed, using cell cultures and in silico models, that weakening intercellular contacts is a fundamental process essential for switching from extensile to contractile tissue behaviour.",
author = "Lakshmi Balasubramaniam and Amin Doostmohammadi and Saw, {Thuan Beng} and Narayana, {Gautham Hari Narayana Sankara} and Romain Mueller and Tien Dang and Minnah Thomas and Shafali Gupta and Surabhi Sonam and Yap, {Alpha S.} and Yusuke Toyama and Rene-Marc Mege and Yeomans, {Julia M.} and Benoit Ladoux",
note = "Correction; https://doi.org/10.1038/s41563-021-00919-2",
year = "2021",
month = feb,
day = "18",
doi = "10.1038/s41563-021-00919-2",
language = "English",
volume = "20",
pages = "1156--1166",
journal = "Nature Materials",
issn = "1476-1122",
publisher = "nature publishing group",
number = "8",

}

RIS

TY - JOUR

T1 - Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers

AU - Balasubramaniam, Lakshmi

AU - Doostmohammadi, Amin

AU - Saw, Thuan Beng

AU - Narayana, Gautham Hari Narayana Sankara

AU - Mueller, Romain

AU - Dang, Tien

AU - Thomas, Minnah

AU - Gupta, Shafali

AU - Sonam, Surabhi

AU - Yap, Alpha S.

AU - Toyama, Yusuke

AU - Mege, Rene-Marc

AU - Yeomans, Julia M.

AU - Ladoux, Benoit

N1 - Correction; https://doi.org/10.1038/s41563-021-00919-2

PY - 2021/2/18

Y1 - 2021/2/18

N2 - Actomyosin machinery endows cells with contractility at a single-cell level. However, within a monolayer, cells can be contractile or extensile based on the direction of pushing or pulling forces exerted by their neighbours or on the substrate. It has been shown that a monolayer of fibroblasts behaves as a contractile system while epithelial or neural progentior monolayers behave as an extensile system. Through a combination of cell culture experiments and in silico modelling, we reveal the mechanism behind this switch in extensile to contractile as the weakening of intercellular contacts. This switch promotes the build-up of tension at the cell-substrate interface through an increase in actin stress fibres and traction forces. This is accompanied by mechanotransductive changes in vinculin and YAP activation. We further show that contractile and extensile differences in cell activity sort cells in mixtures, uncovering a generic mechanism for pattern formation during cell competition, and morphogenesis.It is now revealed, using cell cultures and in silico models, that weakening intercellular contacts is a fundamental process essential for switching from extensile to contractile tissue behaviour.

AB - Actomyosin machinery endows cells with contractility at a single-cell level. However, within a monolayer, cells can be contractile or extensile based on the direction of pushing or pulling forces exerted by their neighbours or on the substrate. It has been shown that a monolayer of fibroblasts behaves as a contractile system while epithelial or neural progentior monolayers behave as an extensile system. Through a combination of cell culture experiments and in silico modelling, we reveal the mechanism behind this switch in extensile to contractile as the weakening of intercellular contacts. This switch promotes the build-up of tension at the cell-substrate interface through an increase in actin stress fibres and traction forces. This is accompanied by mechanotransductive changes in vinculin and YAP activation. We further show that contractile and extensile differences in cell activity sort cells in mixtures, uncovering a generic mechanism for pattern formation during cell competition, and morphogenesis.It is now revealed, using cell cultures and in silico models, that weakening intercellular contacts is a fundamental process essential for switching from extensile to contractile tissue behaviour.

U2 - 10.1038/s41563-021-00919-2

DO - 10.1038/s41563-021-00919-2

M3 - Journal article

C2 - 33603188

VL - 20

SP - 1156

EP - 1166

JO - Nature Materials

JF - Nature Materials

SN - 1476-1122

IS - 8

ER -

ID: 259106696