Integrative analyses of probiotics, pathogenic infections and host immune response highlight the importance of gut microbiota in understanding disease recovery in rainbow trout (Oncorhynchus mykiss)
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Integrative analyses of probiotics, pathogenic infections and host immune response highlight the importance of gut microbiota in understanding disease recovery in rainbow trout (Oncorhynchus mykiss). / Rasmussen, Jacob Agerbo; Villumsen, Kasper Rømer; Jørgensen, Louise von Gersdorff; Forberg, Torunn; Zuo, Shaozhi; Kania, Per Walter; Buchmann, Kurt; Kristiansen, Karsten; Bojesen, Anders Miki; Limborg, Morten Tonsberg.
In: Journal of Applied Microbiology, Vol. 132, No. 4, 2022, p. 3201-3216.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Integrative analyses of probiotics, pathogenic infections and host immune response highlight the importance of gut microbiota in understanding disease recovery in rainbow trout (Oncorhynchus mykiss)
AU - Rasmussen, Jacob Agerbo
AU - Villumsen, Kasper Rømer
AU - Jørgensen, Louise von Gersdorff
AU - Forberg, Torunn
AU - Zuo, Shaozhi
AU - Kania, Per Walter
AU - Buchmann, Kurt
AU - Kristiansen, Karsten
AU - Bojesen, Anders Miki
AU - Limborg, Morten Tonsberg
PY - 2022
Y1 - 2022
N2 - Aims Given the pivotal role played by the gut microbiota in regulating the host immune system, great interest has arisen in the possibility of controlling fish health by modulating the gut microbiota. Hence, there is a need to better understand of the host-microbiota interactions after disease responses to optimize the use of probiotics to strengthen disease resilience and recovery. Methods and Results We tested the effects of a probiotic feed additive in rainbow trout and challenged the fish with the causative agent for enteric red mouth disease, Yersinia ruckeri. We evaluated the survival, host immune gene expression and the gut microbiota composition. Results revealed that provision of probiotics and exposure to Y. ruckeri induced immune gene expression in the host, which were associated with changes in the gut microbiota. Subsequently, infection with Y. ruckeri had very little effect on microbiota composition when probiotics were applied, indicating that probiotics increased stabilisation of the microbiota. Our analysis revealed potential biomarkers for monitoring infection status and fish health. Finally, we used modelling approaches to decipher interactions between gut bacteria and the host immune gene responses, indicating removal of endogenous bacteria elicited by non-specific immune responses. Conclusions We discuss the relevance of these results emphasizing the importance of host-microbiota interactions, including the protective potential of the gut microbiota in disease responses. Significance and Impact of the Study Our results highlight the functional consequences of probiotic-induced changes in the gut microbiota post infection and the resulting host immune response.
AB - Aims Given the pivotal role played by the gut microbiota in regulating the host immune system, great interest has arisen in the possibility of controlling fish health by modulating the gut microbiota. Hence, there is a need to better understand of the host-microbiota interactions after disease responses to optimize the use of probiotics to strengthen disease resilience and recovery. Methods and Results We tested the effects of a probiotic feed additive in rainbow trout and challenged the fish with the causative agent for enteric red mouth disease, Yersinia ruckeri. We evaluated the survival, host immune gene expression and the gut microbiota composition. Results revealed that provision of probiotics and exposure to Y. ruckeri induced immune gene expression in the host, which were associated with changes in the gut microbiota. Subsequently, infection with Y. ruckeri had very little effect on microbiota composition when probiotics were applied, indicating that probiotics increased stabilisation of the microbiota. Our analysis revealed potential biomarkers for monitoring infection status and fish health. Finally, we used modelling approaches to decipher interactions between gut bacteria and the host immune gene responses, indicating removal of endogenous bacteria elicited by non-specific immune responses. Conclusions We discuss the relevance of these results emphasizing the importance of host-microbiota interactions, including the protective potential of the gut microbiota in disease responses. Significance and Impact of the Study Our results highlight the functional consequences of probiotic-induced changes in the gut microbiota post infection and the resulting host immune response.
KW - aquaculture
KW - biomarker predictions
KW - host-microbiota interactions
KW - infection
KW - intestinal microbiology
KW - modelling
KW - probiotics
KW - rainbow trout
KW - Yersinia ruckeri
KW - SALMON SALMO-SALAR
KW - YERSINIA-RUCKERI
KW - VIBRIO-ANGUILLARUM
KW - GENE-EXPRESSION
KW - PCR DATA
KW - COMMUNITIES
U2 - 10.1111/jam.15433
DO - 10.1111/jam.15433
M3 - Journal article
C2 - 35032344
VL - 132
SP - 3201
EP - 3216
JO - Proceedings of the Society for Applied Bacteriology
JF - Proceedings of the Society for Applied Bacteriology
SN - 0266-8254
IS - 4
ER -
ID: 297953277