Increased numbers of orexin/hypocretin neurons in a genetic rat depression model

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Increased numbers of orexin/hypocretin neurons in a genetic rat depression model. / Mikrouli, Elli; Wörtwein, Gitta; Soylu, Rana; Mathé, Aleksander A; Petersén, Åsa.

In: Neuropeptides (Sheffield), Vol. 45, No. 6, 2011, p. 401-6.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Mikrouli, E, Wörtwein, G, Soylu, R, Mathé, AA & Petersén, Å 2011, 'Increased numbers of orexin/hypocretin neurons in a genetic rat depression model', Neuropeptides (Sheffield), vol. 45, no. 6, pp. 401-6. https://doi.org/10.1016/j.npep.2011.07.010

APA

Mikrouli, E., Wörtwein, G., Soylu, R., Mathé, A. A., & Petersén, Å. (2011). Increased numbers of orexin/hypocretin neurons in a genetic rat depression model. Neuropeptides (Sheffield), 45(6), 401-6. https://doi.org/10.1016/j.npep.2011.07.010

Vancouver

Mikrouli E, Wörtwein G, Soylu R, Mathé AA, Petersén Å. Increased numbers of orexin/hypocretin neurons in a genetic rat depression model. Neuropeptides (Sheffield). 2011;45(6):401-6. https://doi.org/10.1016/j.npep.2011.07.010

Author

Mikrouli, Elli ; Wörtwein, Gitta ; Soylu, Rana ; Mathé, Aleksander A ; Petersén, Åsa. / Increased numbers of orexin/hypocretin neurons in a genetic rat depression model. In: Neuropeptides (Sheffield). 2011 ; Vol. 45, No. 6. pp. 401-6.

Bibtex

@article{d2161fc1930043b88b5628c3f1ac301e,
title = "Increased numbers of orexin/hypocretin neurons in a genetic rat depression model",
abstract = "The Flinders Sensitive Line (FSL) rat is a genetic animal model of depression that displays characteristics similar to those of depressed patients including lower body weight, decreased appetite and reduced REM sleep latency. Hypothalamic neuropeptides such as orexin/hypocretin, melanin-concentrating hormone (MCH) and cocaine and amphetamine regulated transcript (CART), that are involved in the regulation of both energy metabolism and sleep, have recently been implicated also in depression. We therefore hypothesized that alterations in these neuropeptide systems may play a role in the development of the FSL phenotype with both depressive like behavior, metabolic abnormalities and sleep disturbances. In this study, we first confirmed that the FSL rats displayed increased immobility in the Porsolt forced swim test compared to their control strain, the Flinders Resistant Line (FRL), which is indicative of depressive-like behavior. We then examined the number of orexin-, MCH- and CART-immunopositive neurons in the hypothalamus using stereological analyses. We found that the total number of orexin-positive neurons was higher in the hypothalamus of female FSL rats compared to female FRL rats, whereas no changes in the MCH or CART populations could be detected between the strains. Chronic treatment with the selective serotonin reuptake inhibitor (SSRI) escitalopram reduced immobility only in the FRL rats where it also increased the number of MCH positive neurons compared to untreated rats. These findings support the view that orexin may be involved in depression and strengthen the notion that the {"}depressed{"} brain responds differently to pharmacological interventions than the normal brain.",
author = "Elli Mikrouli and Gitta W{\"o}rtwein and Rana Soylu and Math{\'e}, {Aleksander A} and {\AA}sa Peters{\'e}n",
note = "Copyright {\textcopyright} 2011 Elsevier Ltd. All rights reserved.",
year = "2011",
doi = "10.1016/j.npep.2011.07.010",
language = "English",
volume = "45",
pages = "401--6",
journal = "Neuropeptides (Sheffield)",
issn = "0142-8233",
number = "6",

}

RIS

TY - JOUR

T1 - Increased numbers of orexin/hypocretin neurons in a genetic rat depression model

AU - Mikrouli, Elli

AU - Wörtwein, Gitta

AU - Soylu, Rana

AU - Mathé, Aleksander A

AU - Petersén, Åsa

N1 - Copyright © 2011 Elsevier Ltd. All rights reserved.

PY - 2011

Y1 - 2011

N2 - The Flinders Sensitive Line (FSL) rat is a genetic animal model of depression that displays characteristics similar to those of depressed patients including lower body weight, decreased appetite and reduced REM sleep latency. Hypothalamic neuropeptides such as orexin/hypocretin, melanin-concentrating hormone (MCH) and cocaine and amphetamine regulated transcript (CART), that are involved in the regulation of both energy metabolism and sleep, have recently been implicated also in depression. We therefore hypothesized that alterations in these neuropeptide systems may play a role in the development of the FSL phenotype with both depressive like behavior, metabolic abnormalities and sleep disturbances. In this study, we first confirmed that the FSL rats displayed increased immobility in the Porsolt forced swim test compared to their control strain, the Flinders Resistant Line (FRL), which is indicative of depressive-like behavior. We then examined the number of orexin-, MCH- and CART-immunopositive neurons in the hypothalamus using stereological analyses. We found that the total number of orexin-positive neurons was higher in the hypothalamus of female FSL rats compared to female FRL rats, whereas no changes in the MCH or CART populations could be detected between the strains. Chronic treatment with the selective serotonin reuptake inhibitor (SSRI) escitalopram reduced immobility only in the FRL rats where it also increased the number of MCH positive neurons compared to untreated rats. These findings support the view that orexin may be involved in depression and strengthen the notion that the "depressed" brain responds differently to pharmacological interventions than the normal brain.

AB - The Flinders Sensitive Line (FSL) rat is a genetic animal model of depression that displays characteristics similar to those of depressed patients including lower body weight, decreased appetite and reduced REM sleep latency. Hypothalamic neuropeptides such as orexin/hypocretin, melanin-concentrating hormone (MCH) and cocaine and amphetamine regulated transcript (CART), that are involved in the regulation of both energy metabolism and sleep, have recently been implicated also in depression. We therefore hypothesized that alterations in these neuropeptide systems may play a role in the development of the FSL phenotype with both depressive like behavior, metabolic abnormalities and sleep disturbances. In this study, we first confirmed that the FSL rats displayed increased immobility in the Porsolt forced swim test compared to their control strain, the Flinders Resistant Line (FRL), which is indicative of depressive-like behavior. We then examined the number of orexin-, MCH- and CART-immunopositive neurons in the hypothalamus using stereological analyses. We found that the total number of orexin-positive neurons was higher in the hypothalamus of female FSL rats compared to female FRL rats, whereas no changes in the MCH or CART populations could be detected between the strains. Chronic treatment with the selective serotonin reuptake inhibitor (SSRI) escitalopram reduced immobility only in the FRL rats where it also increased the number of MCH positive neurons compared to untreated rats. These findings support the view that orexin may be involved in depression and strengthen the notion that the "depressed" brain responds differently to pharmacological interventions than the normal brain.

U2 - 10.1016/j.npep.2011.07.010

DO - 10.1016/j.npep.2011.07.010

M3 - Journal article

C2 - 21871662

VL - 45

SP - 401

EP - 406

JO - Neuropeptides (Sheffield)

JF - Neuropeptides (Sheffield)

SN - 0142-8233

IS - 6

ER -

ID: 37581811