Increased numbers of interleukin-15-expressing cells in active ulcerative colitis

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OBJECTIVES: Interleukin-15 (IL-15) is a novel cytokine sharing many of the activities of IL-2. The goal of this study was to evaluate intracellular and serum IL-15 in ulcerative colitis (UC) and Crohn's disease (CD).

METHODS: Intracellular expression of IL-15 in peripheral blood mononuclear cells (PBMC) from UC patients, CD patients, and controls was studied using cell permeabilization and staining with monoclonal antibodies. Serum levels of IL-15 were detected using ELISA.

RESULTS: Percentage of IL-15 expressing PBMC was increased in UC patients and in five of six of CD patients with moderate and severe disease activity compared with controls. The number of IL-15 expressing cells in patients with active inflammatory bowel disease (IBD) declined within 2 wk of treatment. Serum IL-15 reached detectable levels in 62.5% of UC patients with moderate and severe disease activity but not in UC patients with slight disease activity or in remission, neither in CD patients nor in controls. In vitro lipopolysaccharide (LPS)-induced activation of PBMC from controls was associated with up-regulation of intracellular IL-15 expression (p < 0.01) and release of IL-15.

CONCLUSIONS: UC patients with moderate and severe disease activity have increased percentage of IL-15 expressing PBMC, which might be induced by in vivo cell activation and can lead to elevation of released IL-15 in serum. Increased IL-15 expression after in vitro LPS stimulation of control PBMC suggests a nonspecific production of this cytokine during the immunoinflammatory response.

Original languageEnglish
JournalThe American Journal of Gastroenterology
Issue number9
Pages (from-to)1789-94
Number of pages6
Publication statusPublished - Sep 1996

    Research areas

  • Adult, Case-Control Studies, Colitis, Ulcerative/immunology, Crohn Disease/immunology, Enzyme-Linked Immunosorbent Assay, Female, Gene Expression, Humans, Interleukin-15, Interleukins/metabolism, Leukocytes, Mononuclear/metabolism, Lymphocyte Activation, Male, Time Factors

ID: 218726876