Increased numbers of interleukin-15-expressing cells in active ulcerative colitis
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Increased numbers of interleukin-15-expressing cells in active ulcerative colitis. / Kirman, I; Nielsen, O H.
In: The American Journal of Gastroenterology, Vol. 91, No. 9, 09.1996, p. 1789-94.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Increased numbers of interleukin-15-expressing cells in active ulcerative colitis
AU - Kirman, I
AU - Nielsen, O H
PY - 1996/9
Y1 - 1996/9
N2 - OBJECTIVES: Interleukin-15 (IL-15) is a novel cytokine sharing many of the activities of IL-2. The goal of this study was to evaluate intracellular and serum IL-15 in ulcerative colitis (UC) and Crohn's disease (CD).METHODS: Intracellular expression of IL-15 in peripheral blood mononuclear cells (PBMC) from UC patients, CD patients, and controls was studied using cell permeabilization and staining with monoclonal antibodies. Serum levels of IL-15 were detected using ELISA.RESULTS: Percentage of IL-15 expressing PBMC was increased in UC patients and in five of six of CD patients with moderate and severe disease activity compared with controls. The number of IL-15 expressing cells in patients with active inflammatory bowel disease (IBD) declined within 2 wk of treatment. Serum IL-15 reached detectable levels in 62.5% of UC patients with moderate and severe disease activity but not in UC patients with slight disease activity or in remission, neither in CD patients nor in controls. In vitro lipopolysaccharide (LPS)-induced activation of PBMC from controls was associated with up-regulation of intracellular IL-15 expression (p < 0.01) and release of IL-15.CONCLUSIONS: UC patients with moderate and severe disease activity have increased percentage of IL-15 expressing PBMC, which might be induced by in vivo cell activation and can lead to elevation of released IL-15 in serum. Increased IL-15 expression after in vitro LPS stimulation of control PBMC suggests a nonspecific production of this cytokine during the immunoinflammatory response.
AB - OBJECTIVES: Interleukin-15 (IL-15) is a novel cytokine sharing many of the activities of IL-2. The goal of this study was to evaluate intracellular and serum IL-15 in ulcerative colitis (UC) and Crohn's disease (CD).METHODS: Intracellular expression of IL-15 in peripheral blood mononuclear cells (PBMC) from UC patients, CD patients, and controls was studied using cell permeabilization and staining with monoclonal antibodies. Serum levels of IL-15 were detected using ELISA.RESULTS: Percentage of IL-15 expressing PBMC was increased in UC patients and in five of six of CD patients with moderate and severe disease activity compared with controls. The number of IL-15 expressing cells in patients with active inflammatory bowel disease (IBD) declined within 2 wk of treatment. Serum IL-15 reached detectable levels in 62.5% of UC patients with moderate and severe disease activity but not in UC patients with slight disease activity or in remission, neither in CD patients nor in controls. In vitro lipopolysaccharide (LPS)-induced activation of PBMC from controls was associated with up-regulation of intracellular IL-15 expression (p < 0.01) and release of IL-15.CONCLUSIONS: UC patients with moderate and severe disease activity have increased percentage of IL-15 expressing PBMC, which might be induced by in vivo cell activation and can lead to elevation of released IL-15 in serum. Increased IL-15 expression after in vitro LPS stimulation of control PBMC suggests a nonspecific production of this cytokine during the immunoinflammatory response.
KW - Adult
KW - Case-Control Studies
KW - Colitis, Ulcerative/immunology
KW - Crohn Disease/immunology
KW - Enzyme-Linked Immunosorbent Assay
KW - Female
KW - Gene Expression
KW - Humans
KW - Interleukin-15
KW - Interleukins/metabolism
KW - Leukocytes, Mononuclear/metabolism
KW - Lymphocyte Activation
KW - Male
KW - Time Factors
M3 - Journal article
C2 - 8792700
VL - 91
SP - 1789
EP - 1794
JO - The American Journal of Gastroenterology
JF - The American Journal of Gastroenterology
SN - 0002-9270
IS - 9
ER -
ID: 218726876