Increase in vitamin d but not regulatory t cells following ultraviolet b phototherapy of patients with atopic dermatitis
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Increase in vitamin d but not regulatory t cells following ultraviolet b phototherapy of patients with atopic dermatitis. / Simonsen, Stine; Bonefeld, Charlotte Menné; Thyssen, Jacob Pontoppidan; Geisler, Carsten; Skov, Lone.
In: Acta Dermato-Venereologica, Vol. 99, No. 2, 2019, p. 139-145.Research output: Contribution to journal › Journal article › peer-review
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T1 - Increase in vitamin d but not regulatory t cells following ultraviolet b phototherapy of patients with atopic dermatitis
AU - Simonsen, Stine
AU - Bonefeld, Charlotte Menné
AU - Thyssen, Jacob Pontoppidan
AU - Geisler, Carsten
AU - Skov, Lone
PY - 2019
Y1 - 2019
N2 - This study investigated serum 25-hydroxyvitamin D (25(OH)D) concentrations and circulating regulatory T cells in patients with atopic dermatitis receiving narrow-band ultraviolet B (nbUVB) phototherapy. Thirty adult patients with atopic dermatitis were included. Blood samples were collected at baseline and at weeks 2 and 4 of nbUVB phototherapy. Skin biopsies were taken at baseline and at week 4. Serum 25(OH) D concentrations increased significantly following nbUVB phototherapy (estimate of change from baseline to week 2: 32.00 nmol/l, confidence interval (CI) 20.48–43.52, p < 0.0001, n = 25; and from baseline to week 4: 50.30 nmol/l, CI 37.28–63.33, p < 0.0001, n = 18). This increase was independent of the filaggrin gene loss-of-function mutation status. Flow cytometry showed no significant change in regulatory T cells or cytokine profiles of T cells in blood. Real-time quantitative PCR showed no change in skin cytokine levels. In conclusion, nbUVB phototherapy was associated with increased serum 25(OH)D concentrations, but not changes in circulating regulatory T cells in patients with atopic dermatitis.
AB - This study investigated serum 25-hydroxyvitamin D (25(OH)D) concentrations and circulating regulatory T cells in patients with atopic dermatitis receiving narrow-band ultraviolet B (nbUVB) phototherapy. Thirty adult patients with atopic dermatitis were included. Blood samples were collected at baseline and at weeks 2 and 4 of nbUVB phototherapy. Skin biopsies were taken at baseline and at week 4. Serum 25(OH) D concentrations increased significantly following nbUVB phototherapy (estimate of change from baseline to week 2: 32.00 nmol/l, confidence interval (CI) 20.48–43.52, p < 0.0001, n = 25; and from baseline to week 4: 50.30 nmol/l, CI 37.28–63.33, p < 0.0001, n = 18). This increase was independent of the filaggrin gene loss-of-function mutation status. Flow cytometry showed no significant change in regulatory T cells or cytokine profiles of T cells in blood. Real-time quantitative PCR showed no change in skin cytokine levels. In conclusion, nbUVB phototherapy was associated with increased serum 25(OH)D concentrations, but not changes in circulating regulatory T cells in patients with atopic dermatitis.
KW - 25-hydroxyvitamin D
KW - Atopic dermatitis
KW - Filaggrin
KW - NbUVB phototherapy
KW - Regulatory T cells
U2 - 10.2340/00015555-3050
DO - 10.2340/00015555-3050
M3 - Journal article
C2 - 30250963
AN - SCOPUS:85060957801
VL - 99
SP - 139
EP - 145
JO - Acta Dermato-Venereologica
JF - Acta Dermato-Venereologica
SN - 0001-5555
IS - 2
ER -
ID: 216872777