In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

CRISPR/Cas9 technology allows facile modification of the genome in virtually any desired way through the use of easily designed plasmid constructs that express a gRNA targeting a genomic site-of-interest and Cas9. Hydrodynamic tail vein injection, on the other hand, is a simple method to deliver "naked" plasmid DNA to 5-40% of the hepatocytes of the liver of adult mice. Here, we describe how these two techniques can be combined to create a workflow for fast, easy, and cost-efficient in vivo genome editing of the adult mouse liver. Using this method, large cohorts of mice with genetically modified livers can be established within 3 weeks to generate models for gene function in normal physiology and diseases of the liver.

Original languageEnglish
Title of host publicationCRISPR Gene Editing : Methods and Protocols
EditorsYonglun Luo
Number of pages13
Place of PublicationNew York, NY
PublisherHumana Press
Publication date2019
Article number20
ISBN (Print)978-1-4939-9169-3
ISBN (Electronic)978-1-4939-9170-9
Publication statusPublished - 2019
SeriesMethods in molecular biology (Clifton, N.J.)

    Research areas

  • Animals, CRISPR-Cas Systems/genetics, Gene Editing, Hepatocytes/metabolism, Liver/metabolism, Mice, Plasmids/genetics, RNA, Guide/genetics

ID: 231415213