In vivo activity of cationic immune stimulating complexes (PLUSCOMs)

Research output: Contribution to journal › Journal article › peer-review

Standard

In vivo activity of cationic immune stimulating complexes (PLUSCOMs). / McBurney, Warren T; Lendemans, Dirk G; Myschik, Julia; Hennessy, Tania; Rades, Thomas; Hook, Sarah.

In: Vaccine, Vol. 26, No. 35, 2008, p. 4549-56.

Research output: Contribution to journal › Journal article › peer-review

Harvard

McBurney, WT, Lendemans, DG, Myschik, J, Hennessy, T, Rades, T & Hook, S 2008, 'In vivo activity of cationic immune stimulating complexes (PLUSCOMs)', Vaccine, vol. 26, no. 35, pp. 4549-56. https://doi.org/10.1016/j.vaccine.2008.06.024

APA

McBurney, W. T., Lendemans, D. G., Myschik, J., Hennessy, T., Rades, T., & Hook, S. (2008). In vivo activity of cationic immune stimulating complexes (PLUSCOMs). Vaccine, 26(35), 4549-56. https://doi.org/10.1016/j.vaccine.2008.06.024

Vancouver

McBurney WT, Lendemans DG, Myschik J, Hennessy T, Rades T, Hook S. In vivo activity of cationic immune stimulating complexes (PLUSCOMs). Vaccine. 2008;26(35):4549-56. https://doi.org/10.1016/j.vaccine.2008.06.024

Author

McBurney, Warren T ; Lendemans, Dirk G ; Myschik, Julia ; Hennessy, Tania ; Rades, Thomas ; Hook, Sarah. / In vivo activity of cationic immune stimulating complexes (PLUSCOMs). In: Vaccine. 2008 ; Vol. 26, No. 35. pp. 4549-56.

Bibtex

@article{7b776bd79bda4278a95b7f4515c77726,
title = "In vivo activity of cationic immune stimulating complexes (PLUSCOMs)",
abstract = "A particulate vaccine delivery system consisting of cationic ISCOM derivatives (PLUSCOMs) was compared to classic anionic ISCOMs with regard to antigen attachment and ability to elicit in vivo T cell responses against a model protein antigen (ovalbumin [OVA]). ISCOMs did not incorporate hydrophilic OVA whilst OVA readily adsorbed onto PLUSCOMs with increasing adsorption at higher protein concentrations. The zeta-potential of PLUSCOMs significantly decreased with increasing protein load, suggesting neutralization of the cationic charge upon absorption of the anionic OVA. Antigen-specific CD8 T cell responses were demonstrated in mice vaccinated with either PLUSCOMs or ISCOMs. Ex vivo restimulation of harvested T cells demonstrated that cells isolated from PLUSCOM and ISCOM vaccinated mice responded to the secondary OVA challenge more efficiently than mice vaccinated with OVA in solution. Restimulated cells from the mice vaccinated with particulate vaccines produced significantly more INF-gamma. Therefore PLUSCOMs are as effective as classic ISCOMs in inducing antigen-specific CD8 T cell responses and have advantages with regard to the incorporation of purified anionic antigens.",
author = "McBurney, {Warren T} and Lendemans, {Dirk G} and Julia Myschik and Tania Hennessy and Thomas Rades and Sarah Hook",
year = "2008",
doi = "10.1016/j.vaccine.2008.06.024",
language = "English",
volume = "26",
pages = "4549--56",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier",
number = "35",

}

RIS

TY - JOUR

T1 - In vivo activity of cationic immune stimulating complexes (PLUSCOMs)

AU - McBurney, Warren T

AU - Lendemans, Dirk G

AU - Myschik, Julia

AU - Hennessy, Tania

AU - Rades, Thomas

AU - Hook, Sarah

PY - 2008

Y1 - 2008

N2 - A particulate vaccine delivery system consisting of cationic ISCOM derivatives (PLUSCOMs) was compared to classic anionic ISCOMs with regard to antigen attachment and ability to elicit in vivo T cell responses against a model protein antigen (ovalbumin [OVA]). ISCOMs did not incorporate hydrophilic OVA whilst OVA readily adsorbed onto PLUSCOMs with increasing adsorption at higher protein concentrations. The zeta-potential of PLUSCOMs significantly decreased with increasing protein load, suggesting neutralization of the cationic charge upon absorption of the anionic OVA. Antigen-specific CD8 T cell responses were demonstrated in mice vaccinated with either PLUSCOMs or ISCOMs. Ex vivo restimulation of harvested T cells demonstrated that cells isolated from PLUSCOM and ISCOM vaccinated mice responded to the secondary OVA challenge more efficiently than mice vaccinated with OVA in solution. Restimulated cells from the mice vaccinated with particulate vaccines produced significantly more INF-gamma. Therefore PLUSCOMs are as effective as classic ISCOMs in inducing antigen-specific CD8 T cell responses and have advantages with regard to the incorporation of purified anionic antigens.

AB - A particulate vaccine delivery system consisting of cationic ISCOM derivatives (PLUSCOMs) was compared to classic anionic ISCOMs with regard to antigen attachment and ability to elicit in vivo T cell responses against a model protein antigen (ovalbumin [OVA]). ISCOMs did not incorporate hydrophilic OVA whilst OVA readily adsorbed onto PLUSCOMs with increasing adsorption at higher protein concentrations. The zeta-potential of PLUSCOMs significantly decreased with increasing protein load, suggesting neutralization of the cationic charge upon absorption of the anionic OVA. Antigen-specific CD8 T cell responses were demonstrated in mice vaccinated with either PLUSCOMs or ISCOMs. Ex vivo restimulation of harvested T cells demonstrated that cells isolated from PLUSCOM and ISCOM vaccinated mice responded to the secondary OVA challenge more efficiently than mice vaccinated with OVA in solution. Restimulated cells from the mice vaccinated with particulate vaccines produced significantly more INF-gamma. Therefore PLUSCOMs are as effective as classic ISCOMs in inducing antigen-specific CD8 T cell responses and have advantages with regard to the incorporation of purified anionic antigens.

U2 - 10.1016/j.vaccine.2008.06.024

DO - 10.1016/j.vaccine.2008.06.024

M3 - Journal article

C2 - 18585421

VL - 26

SP - 4549

EP - 4556

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 35

ER -

ID: 40353340