In Vitro Palmitate Treatment of Myotubes from Postmenopausal Women Leads to Ceramide Accumulation, Inflammation and Affected Insulin Signaling

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In Vitro Palmitate Treatment of Myotubes from Postmenopausal Women Leads to Ceramide Accumulation, Inflammation and Affected Insulin Signaling. / Abildgaard, Julie; Henstridge, Darren C; Pedersen, Anette Tønnes; Langley, Katherine G; Scheele, Camilla; Pedersen, Bente Klarlund; Lindegaard, Birgitte.

In: PLOS ONE, Vol. 9, No. 7, e101555, 2014, p. 1-11.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Abildgaard, J, Henstridge, DC, Pedersen, AT, Langley, KG, Scheele, C, Pedersen, BK & Lindegaard, B 2014, 'In Vitro Palmitate Treatment of Myotubes from Postmenopausal Women Leads to Ceramide Accumulation, Inflammation and Affected Insulin Signaling', PLOS ONE, vol. 9, no. 7, e101555, pp. 1-11. https://doi.org/10.1371/journal.pone.0101555

APA

Abildgaard, J., Henstridge, D. C., Pedersen, A. T., Langley, K. G., Scheele, C., Pedersen, B. K., & Lindegaard, B. (2014). In Vitro Palmitate Treatment of Myotubes from Postmenopausal Women Leads to Ceramide Accumulation, Inflammation and Affected Insulin Signaling. PLOS ONE, 9(7), 1-11. [e101555]. https://doi.org/10.1371/journal.pone.0101555

Vancouver

Abildgaard J, Henstridge DC, Pedersen AT, Langley KG, Scheele C, Pedersen BK et al. In Vitro Palmitate Treatment of Myotubes from Postmenopausal Women Leads to Ceramide Accumulation, Inflammation and Affected Insulin Signaling. PLOS ONE. 2014;9(7):1-11. e101555. https://doi.org/10.1371/journal.pone.0101555

Author

Abildgaard, Julie ; Henstridge, Darren C ; Pedersen, Anette Tønnes ; Langley, Katherine G ; Scheele, Camilla ; Pedersen, Bente Klarlund ; Lindegaard, Birgitte. / In Vitro Palmitate Treatment of Myotubes from Postmenopausal Women Leads to Ceramide Accumulation, Inflammation and Affected Insulin Signaling. In: PLOS ONE. 2014 ; Vol. 9, No. 7. pp. 1-11.

Bibtex

@article{7cda4ce251fd4b9c8c3a242d3b6da416,
title = "In Vitro Palmitate Treatment of Myotubes from Postmenopausal Women Leads to Ceramide Accumulation, Inflammation and Affected Insulin Signaling",
abstract = "Menopause is associated with an increased incidence of insulin resistance and metabolic diseases. In a chronic palmitate treatment model, we investigated the role of skeletal muscle fatty acid exposure in relation to the metabolic deterioration observed with menopause. Human skeletal muscle satellite cells were isolated from premenopausal (n = 6) and postmenopausal (n = 5) women. In an in vitro model, the myotubes were treated with palmitate (300 µM) for one-, two- or three days during differentiation. Effects on lipid accumulation, inflammation and insulin signaling were studied. Palmitate treatment led to a 108{\%} (CI 95{\%}: 50{\%}; 267{\%}) increase in intramyocellular ceramide in the myotubes from the postmenopausal women (post-myotubes) compared with a 26{\%} (CI 95{\%}: -57{\%}; 96{\%}) increase in myotubes from the premenopausal women (pre-myotubes), (p<0.05). Furthermore, post-myotubes had a 22{\%} (CI 95{\%}: 4{\%}; 34{\%}) increase in pJNK (p = 0.04) and a 114{\%} (CI 95{\%}: 50{\%}; 177{\%}) increase in Hsp70 protein expression (p = 0.03) after three days of palmitate treatment, compared with pre-myotubes, in which no increase in either pJNK (-12{\%} (CI 95: -26{\%}; 2{\%})) or Hsp70 (7{\%} (CI 95: -78{\%}; 91{\%})) was detected. Furthermore, post-myotubes showed a blunted insulin stimulated phosphorylation of AS160 in response to chronic palmitate treatment compared with pre-myotubes (p = 0.02). The increased intramyocellular ceramide content in the post-myotubes was associated with a significantly higher mRNA expression of Serine Palmitoyltransferase1 (SPT1) after one day of palmitate treatment (p = 0.03) in post-myotubes compared with pre-myotubes. Our findings indicate that post-myotubes are more prone to develop lipid accumulation and defective insulin signaling following chronic saturated fatty acid exposure as compared to pre-myotubes.",
keywords = "Adipose Tissue, Ceramides, Female, HSP70 Heat-Shock Proteins, Humans, Insulin, Insulin Resistance, JNK Mitogen-Activated Protein Kinases, Lipid Metabolism, Middle Aged, Muscle Fibers, Skeletal, Oxidative Stress, Palmitates, Phosphoproteins, Postmenopause, Signal Transduction",
author = "Julie Abildgaard and Henstridge, {Darren C} and Pedersen, {Anette T{\o}nnes} and Langley, {Katherine G} and Camilla Scheele and Pedersen, {Bente Klarlund} and Birgitte Lindegaard",
year = "2014",
doi = "10.1371/journal.pone.0101555",
language = "English",
volume = "9",
pages = "1--11",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "7",

}

RIS

TY - JOUR

T1 - In Vitro Palmitate Treatment of Myotubes from Postmenopausal Women Leads to Ceramide Accumulation, Inflammation and Affected Insulin Signaling

AU - Abildgaard, Julie

AU - Henstridge, Darren C

AU - Pedersen, Anette Tønnes

AU - Langley, Katherine G

AU - Scheele, Camilla

AU - Pedersen, Bente Klarlund

AU - Lindegaard, Birgitte

PY - 2014

Y1 - 2014

N2 - Menopause is associated with an increased incidence of insulin resistance and metabolic diseases. In a chronic palmitate treatment model, we investigated the role of skeletal muscle fatty acid exposure in relation to the metabolic deterioration observed with menopause. Human skeletal muscle satellite cells were isolated from premenopausal (n = 6) and postmenopausal (n = 5) women. In an in vitro model, the myotubes were treated with palmitate (300 µM) for one-, two- or three days during differentiation. Effects on lipid accumulation, inflammation and insulin signaling were studied. Palmitate treatment led to a 108% (CI 95%: 50%; 267%) increase in intramyocellular ceramide in the myotubes from the postmenopausal women (post-myotubes) compared with a 26% (CI 95%: -57%; 96%) increase in myotubes from the premenopausal women (pre-myotubes), (p<0.05). Furthermore, post-myotubes had a 22% (CI 95%: 4%; 34%) increase in pJNK (p = 0.04) and a 114% (CI 95%: 50%; 177%) increase in Hsp70 protein expression (p = 0.03) after three days of palmitate treatment, compared with pre-myotubes, in which no increase in either pJNK (-12% (CI 95: -26%; 2%)) or Hsp70 (7% (CI 95: -78%; 91%)) was detected. Furthermore, post-myotubes showed a blunted insulin stimulated phosphorylation of AS160 in response to chronic palmitate treatment compared with pre-myotubes (p = 0.02). The increased intramyocellular ceramide content in the post-myotubes was associated with a significantly higher mRNA expression of Serine Palmitoyltransferase1 (SPT1) after one day of palmitate treatment (p = 0.03) in post-myotubes compared with pre-myotubes. Our findings indicate that post-myotubes are more prone to develop lipid accumulation and defective insulin signaling following chronic saturated fatty acid exposure as compared to pre-myotubes.

AB - Menopause is associated with an increased incidence of insulin resistance and metabolic diseases. In a chronic palmitate treatment model, we investigated the role of skeletal muscle fatty acid exposure in relation to the metabolic deterioration observed with menopause. Human skeletal muscle satellite cells were isolated from premenopausal (n = 6) and postmenopausal (n = 5) women. In an in vitro model, the myotubes were treated with palmitate (300 µM) for one-, two- or three days during differentiation. Effects on lipid accumulation, inflammation and insulin signaling were studied. Palmitate treatment led to a 108% (CI 95%: 50%; 267%) increase in intramyocellular ceramide in the myotubes from the postmenopausal women (post-myotubes) compared with a 26% (CI 95%: -57%; 96%) increase in myotubes from the premenopausal women (pre-myotubes), (p<0.05). Furthermore, post-myotubes had a 22% (CI 95%: 4%; 34%) increase in pJNK (p = 0.04) and a 114% (CI 95%: 50%; 177%) increase in Hsp70 protein expression (p = 0.03) after three days of palmitate treatment, compared with pre-myotubes, in which no increase in either pJNK (-12% (CI 95: -26%; 2%)) or Hsp70 (7% (CI 95: -78%; 91%)) was detected. Furthermore, post-myotubes showed a blunted insulin stimulated phosphorylation of AS160 in response to chronic palmitate treatment compared with pre-myotubes (p = 0.02). The increased intramyocellular ceramide content in the post-myotubes was associated with a significantly higher mRNA expression of Serine Palmitoyltransferase1 (SPT1) after one day of palmitate treatment (p = 0.03) in post-myotubes compared with pre-myotubes. Our findings indicate that post-myotubes are more prone to develop lipid accumulation and defective insulin signaling following chronic saturated fatty acid exposure as compared to pre-myotubes.

KW - Adipose Tissue

KW - Ceramides

KW - Female

KW - HSP70 Heat-Shock Proteins

KW - Humans

KW - Insulin

KW - Insulin Resistance

KW - JNK Mitogen-Activated Protein Kinases

KW - Lipid Metabolism

KW - Middle Aged

KW - Muscle Fibers, Skeletal

KW - Oxidative Stress

KW - Palmitates

KW - Phosphoproteins

KW - Postmenopause

KW - Signal Transduction

U2 - 10.1371/journal.pone.0101555

DO - 10.1371/journal.pone.0101555

M3 - Journal article

C2 - 25000528

VL - 9

SP - 1

EP - 11

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 7

M1 - e101555

ER -

ID: 138138867