In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice
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In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice. / Fang, Evandro F.; Palikaras, Konstantinos; Sun, Nuo; Fivenson, Elayne M.; Spangler, Ryan D.; Kerr, Jesse S.; Cordonnier, Stephanie A.; Hou, Yujun; Dombi, Eszter; Kassahun, Henok; Tavernarakis, Nektarios; Poulton, Joanna; Nilsen, Hilde; Bohr, Vilhelm A.
In: Journal of Visualized Experiments, No. 129, e56301, 2017.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice
AU - Fang, Evandro F.
AU - Palikaras, Konstantinos
AU - Sun, Nuo
AU - Fivenson, Elayne M.
AU - Spangler, Ryan D.
AU - Kerr, Jesse S.
AU - Cordonnier, Stephanie A.
AU - Hou, Yujun
AU - Dombi, Eszter
AU - Kassahun, Henok
AU - Tavernarakis, Nektarios
AU - Poulton, Joanna
AU - Nilsen, Hilde
AU - Bohr, Vilhelm A.
PY - 2017
Y1 - 2017
N2 - Mitochondria are the powerhouses of cells and produce cellular energy in the form of ATP. Mitochondrial dysfunction contributes to biological aging and a wide variety of disorders including metabolic diseases, premature aging syndromes, and neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). Maintenance of mitochondrial health depends on mitochondrial biogenesis and the efficient clearance of dysfunctional mitochondria through mitophagy. Experimental methods to accurately detect autophagy/mitophagy, especially in animal models, have been challenging to develop. Recent progress towards the understanding of the molecular mechanisms of mitophagy has enabled the development of novel mitophagy detection techniques. Here, we introduce several versatile techniques to monitor mitophagy in human cells, Caenorhabditis elegans (e.g., Rosella and DCT-1/ LGG-1 strains), and mice (mt-Keima). A combination of these mitophagy detection techniques, including cross-species evaluation, will improve the accuracy of mitophagy measurements and lead to a better understanding of the role of mitophagy in health and disease.
AB - Mitochondria are the powerhouses of cells and produce cellular energy in the form of ATP. Mitochondrial dysfunction contributes to biological aging and a wide variety of disorders including metabolic diseases, premature aging syndromes, and neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). Maintenance of mitochondrial health depends on mitochondrial biogenesis and the efficient clearance of dysfunctional mitochondria through mitophagy. Experimental methods to accurately detect autophagy/mitophagy, especially in animal models, have been challenging to develop. Recent progress towards the understanding of the molecular mechanisms of mitophagy has enabled the development of novel mitophagy detection techniques. Here, we introduce several versatile techniques to monitor mitophagy in human cells, Caenorhabditis elegans (e.g., Rosella and DCT-1/ LGG-1 strains), and mice (mt-Keima). A combination of these mitophagy detection techniques, including cross-species evaluation, will improve the accuracy of mitophagy measurements and lead to a better understanding of the role of mitophagy in health and disease.
U2 - 10.3791/56301
DO - 10.3791/56301
M3 - Journal article
C2 - 29286376
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
SN - 1940-087X
IS - 129
M1 - e56301
ER -
ID: 197439466