In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Research output: Contribution to journalJournal articleResearchpeer-review

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In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice. / Fang, Evandro F.; Palikaras, Konstantinos; Sun, Nuo; Fivenson, Elayne M.; Spangler, Ryan D.; Kerr, Jesse S.; Cordonnier, Stephanie A.; Hou, Yujun; Dombi, Eszter; Kassahun, Henok; Tavernarakis, Nektarios; Poulton, Joanna; Nilsen, Hilde; Bohr, Vilhelm A.

In: Journal of Visualized Experiments, No. 129, e56301, 2017.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Fang, EF, Palikaras, K, Sun, N, Fivenson, EM, Spangler, RD, Kerr, JS, Cordonnier, SA, Hou, Y, Dombi, E, Kassahun, H, Tavernarakis, N, Poulton, J, Nilsen, H & Bohr, VA 2017, 'In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice', Journal of Visualized Experiments, no. 129, e56301. https://doi.org/10.3791/56301

APA

Fang, E. F., Palikaras, K., Sun, N., Fivenson, E. M., Spangler, R. D., Kerr, J. S., ... Bohr, V. A. (2017). In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice. Journal of Visualized Experiments, (129), [e56301]. https://doi.org/10.3791/56301

Vancouver

Fang EF, Palikaras K, Sun N, Fivenson EM, Spangler RD, Kerr JS et al. In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice. Journal of Visualized Experiments. 2017;(129). e56301. https://doi.org/10.3791/56301

Author

Fang, Evandro F. ; Palikaras, Konstantinos ; Sun, Nuo ; Fivenson, Elayne M. ; Spangler, Ryan D. ; Kerr, Jesse S. ; Cordonnier, Stephanie A. ; Hou, Yujun ; Dombi, Eszter ; Kassahun, Henok ; Tavernarakis, Nektarios ; Poulton, Joanna ; Nilsen, Hilde ; Bohr, Vilhelm A. / In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice. In: Journal of Visualized Experiments. 2017 ; No. 129.

Bibtex

@article{23eb06911b6b4969b2ff1729b898706e,
title = "In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice",
abstract = "Mitochondria are the powerhouses of cells and produce cellular energy in the form of ATP. Mitochondrial dysfunction contributes to biological aging and a wide variety of disorders including metabolic diseases, premature aging syndromes, and neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). Maintenance of mitochondrial health depends on mitochondrial biogenesis and the efficient clearance of dysfunctional mitochondria through mitophagy. Experimental methods to accurately detect autophagy/mitophagy, especially in animal models, have been challenging to develop. Recent progress towards the understanding of the molecular mechanisms of mitophagy has enabled the development of novel mitophagy detection techniques. Here, we introduce several versatile techniques to monitor mitophagy in human cells, Caenorhabditis elegans (e.g., Rosella and DCT-1/ LGG-1 strains), and mice (mt-Keima). A combination of these mitophagy detection techniques, including cross-species evaluation, will improve the accuracy of mitophagy measurements and lead to a better understanding of the role of mitophagy in health and disease.",
author = "Fang, {Evandro F.} and Konstantinos Palikaras and Nuo Sun and Fivenson, {Elayne M.} and Spangler, {Ryan D.} and Kerr, {Jesse S.} and Cordonnier, {Stephanie A.} and Yujun Hou and Eszter Dombi and Henok Kassahun and Nektarios Tavernarakis and Joanna Poulton and Hilde Nilsen and Bohr, {Vilhelm A.}",
year = "2017",
doi = "10.3791/56301",
language = "English",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "Journal of Visualized Experiments",
number = "129",

}

RIS

TY - JOUR

T1 - In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

AU - Fang, Evandro F.

AU - Palikaras, Konstantinos

AU - Sun, Nuo

AU - Fivenson, Elayne M.

AU - Spangler, Ryan D.

AU - Kerr, Jesse S.

AU - Cordonnier, Stephanie A.

AU - Hou, Yujun

AU - Dombi, Eszter

AU - Kassahun, Henok

AU - Tavernarakis, Nektarios

AU - Poulton, Joanna

AU - Nilsen, Hilde

AU - Bohr, Vilhelm A.

PY - 2017

Y1 - 2017

N2 - Mitochondria are the powerhouses of cells and produce cellular energy in the form of ATP. Mitochondrial dysfunction contributes to biological aging and a wide variety of disorders including metabolic diseases, premature aging syndromes, and neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). Maintenance of mitochondrial health depends on mitochondrial biogenesis and the efficient clearance of dysfunctional mitochondria through mitophagy. Experimental methods to accurately detect autophagy/mitophagy, especially in animal models, have been challenging to develop. Recent progress towards the understanding of the molecular mechanisms of mitophagy has enabled the development of novel mitophagy detection techniques. Here, we introduce several versatile techniques to monitor mitophagy in human cells, Caenorhabditis elegans (e.g., Rosella and DCT-1/ LGG-1 strains), and mice (mt-Keima). A combination of these mitophagy detection techniques, including cross-species evaluation, will improve the accuracy of mitophagy measurements and lead to a better understanding of the role of mitophagy in health and disease.

AB - Mitochondria are the powerhouses of cells and produce cellular energy in the form of ATP. Mitochondrial dysfunction contributes to biological aging and a wide variety of disorders including metabolic diseases, premature aging syndromes, and neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). Maintenance of mitochondrial health depends on mitochondrial biogenesis and the efficient clearance of dysfunctional mitochondria through mitophagy. Experimental methods to accurately detect autophagy/mitophagy, especially in animal models, have been challenging to develop. Recent progress towards the understanding of the molecular mechanisms of mitophagy has enabled the development of novel mitophagy detection techniques. Here, we introduce several versatile techniques to monitor mitophagy in human cells, Caenorhabditis elegans (e.g., Rosella and DCT-1/ LGG-1 strains), and mice (mt-Keima). A combination of these mitophagy detection techniques, including cross-species evaluation, will improve the accuracy of mitophagy measurements and lead to a better understanding of the role of mitophagy in health and disease.

U2 - 10.3791/56301

DO - 10.3791/56301

M3 - Journal article

C2 - 29286376

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 129

M1 - e56301

ER -

ID: 197439466