Impact of Pseudomonas H6 surfactant on all external life cycle stages of the fish parasitic ciliate Ichthyophthirius multifiliis
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Impact of Pseudomonas H6 surfactant on all external life cycle stages of the fish parasitic ciliate Ichthyophthirius multifiliis. / Al-Jubury, Azmi; Lu, C.; Kania, Per Walter; Jørgensen, Louise von Gersdorff; Liu, Y.; de Bruijn, I.; Raaijmakers, J.; Buchmann, Kurt.
In: Journal of Fish Diseases, Vol. 41, No. 7, 2018, p. 1147-1152.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Impact of Pseudomonas H6 surfactant on all external life cycle stages of the fish parasitic ciliate Ichthyophthirius multifiliis
AU - Al-Jubury, Azmi
AU - Lu, C.
AU - Kania, Per Walter
AU - Jørgensen, Louise von Gersdorff
AU - Liu, Y.
AU - de Bruijn, I.
AU - Raaijmakers, J.
AU - Buchmann, Kurt
PY - 2018
Y1 - 2018
N2 - A bacterial biosurfactant isolated from Pseudomonas (strain H6) has previously been shown to have a lethal effect on the oomycete Saprolegnia diclina infecting fish eggs. The present work demonstrates that the same biosurfactant has a strong in vitro antiparasitic effect on the fish pathogenic ciliate Ichthyophthirius multifiliis. Three life cycle stages (the infective theront stage, the tomont and the tomocyst containing tomites) were all susceptible to the surfactant. Theronts were the most sensitive showing 100% mortality in as low concentrations as 10 and 13 μg/ml within 30 min. Tomonts were the most resistant but were killed in concentrations of 100 μg/ml. Tomocysts, which generally are considered resistant to chemical and medical treatment, due to the surrounding protective cyst wall, were also sensitive. The surfactant, in concentrations of 10 and 13 μg/ml, penetrated the cyst wall and killed the enclosed tomites within 60 min. Rainbow trout fingerlings exposed to the biosurfactant showed no adverse immediate or late signs following several hours incubation in concentrations effective for killing the parasite. This bacterial surfactant may be further developed for application as an antiparasitic control agent in aquaculture.
AB - A bacterial biosurfactant isolated from Pseudomonas (strain H6) has previously been shown to have a lethal effect on the oomycete Saprolegnia diclina infecting fish eggs. The present work demonstrates that the same biosurfactant has a strong in vitro antiparasitic effect on the fish pathogenic ciliate Ichthyophthirius multifiliis. Three life cycle stages (the infective theront stage, the tomont and the tomocyst containing tomites) were all susceptible to the surfactant. Theronts were the most sensitive showing 100% mortality in as low concentrations as 10 and 13 μg/ml within 30 min. Tomonts were the most resistant but were killed in concentrations of 100 μg/ml. Tomocysts, which generally are considered resistant to chemical and medical treatment, due to the surrounding protective cyst wall, were also sensitive. The surfactant, in concentrations of 10 and 13 μg/ml, penetrated the cyst wall and killed the enclosed tomites within 60 min. Rainbow trout fingerlings exposed to the biosurfactant showed no adverse immediate or late signs following several hours incubation in concentrations effective for killing the parasite. This bacterial surfactant may be further developed for application as an antiparasitic control agent in aquaculture.
KW - biological control
KW - Ichthyophthirius multifiliis
KW - parasiticide
KW - Pseudomonas
KW - surfactant
U2 - 10.1111/jfd.12810
DO - 10.1111/jfd.12810
M3 - Journal article
C2 - 29671884
AN - SCOPUS:85048362462
VL - 41
SP - 1147
EP - 1152
JO - Journal of Fish Diseases
JF - Journal of Fish Diseases
SN - 0140-7775
IS - 7
ER -
ID: 201154193