Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity
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Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity. / Fenton, Thomas M.; Jørgensen, Peter B.; Niss, Kristoffer; Rubin, Samuel J.S.; Mörbe, Urs M.; Riis, Lene B.; Da Silva, Clément; Plumb, Adam; Vandamme, Julien; Jakobsen, Henrik L.; Brunak, Søren; Habtezion, Aida; Nielsen, Ole H.; Johansson-Lindbom, Bengt; Agace, William W.
In: Immunity, Vol. 52, No. 3, 2020, p. 557-570, e1-e6.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity
AU - Fenton, Thomas M.
AU - Jørgensen, Peter B.
AU - Niss, Kristoffer
AU - Rubin, Samuel J.S.
AU - Mörbe, Urs M.
AU - Riis, Lene B.
AU - Da Silva, Clément
AU - Plumb, Adam
AU - Vandamme, Julien
AU - Jakobsen, Henrik L.
AU - Brunak, Søren
AU - Habtezion, Aida
AU - Nielsen, Ole H.
AU - Johansson-Lindbom, Bengt
AU - Agace, William W.
PY - 2020
Y1 - 2020
N2 - The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1+ high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal adaptive immune responses are initiated in an anatomically restricted manner. Our findings position ILFs as key inductive hubs for regional immunity in the human intestine, and the methods presented will allow future assessment of these compartments in health and disease.
AB - The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1+ high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal adaptive immune responses are initiated in an anatomically restricted manner. Our findings position ILFs as key inductive hubs for regional immunity in the human intestine, and the methods presented will allow future assessment of these compartments in health and disease.
KW - colon
KW - human intestinal regional mucosal follicles
KW - IgA
KW - iIeum
KW - ILF
KW - PP
U2 - 10.1016/j.immuni.2020.02.001
DO - 10.1016/j.immuni.2020.02.001
M3 - Journal article
C2 - 32160523
AN - SCOPUS:85081279962
VL - 52
SP - 557-570, e1-e6
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 3
ER -
ID: 237999668