Identification of minimal sequences of the Rhopalosiphum padi virus 5' untranslated region required for internal initiation of protein synthesis in mammalian, plant and insect translation systems
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Identification of minimal sequences of the Rhopalosiphum padi virus 5' untranslated region required for internal initiation of protein synthesis in mammalian, plant and insect translation systems. / Groppelli, Elisabetta; Belsham, Graham J; Roberts, Lisa O.
In: The Journal of general virology, Vol. 88, No. Pt 5, 05.2007, p. 1583-1588.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Identification of minimal sequences of the Rhopalosiphum padi virus 5' untranslated region required for internal initiation of protein synthesis in mammalian, plant and insect translation systems
AU - Groppelli, Elisabetta
AU - Belsham, Graham J
AU - Roberts, Lisa O
PY - 2007/5
Y1 - 2007/5
N2 - Rhopalosiphum padi virus (RhPV) is a member of the family Dicistroviridae. The genomes of viruses in this family contain two open reading frames, each preceded by distinct internal ribosome entry site (IRES) elements. The RhPV 5' IRES is functional in mammalian, insect and plant translation systems and can form 48S initiation complexes in vitro with just the mammalian initiation factors eIF2, eIF3 and eIF1. Large regions of the 5' untranslated region (UTR) can be deleted without affecting initiation-complex formation. The minimal sequences required for directing internal initiation in mammalian (rabbit reticulocyte lysate), plant (wheatgerm extract) and insect (Sf21 cells) translation systems have now been defined. A fragment (nt 426-579) from the 3' portion of the 5' UTR can direct translation in each of these translation systems. In addition, a distinct region (nt 300-429) is also active. Thus, unstructured regions within the 5' UTR seem to be critical for IRES function.
AB - Rhopalosiphum padi virus (RhPV) is a member of the family Dicistroviridae. The genomes of viruses in this family contain two open reading frames, each preceded by distinct internal ribosome entry site (IRES) elements. The RhPV 5' IRES is functional in mammalian, insect and plant translation systems and can form 48S initiation complexes in vitro with just the mammalian initiation factors eIF2, eIF3 and eIF1. Large regions of the 5' untranslated region (UTR) can be deleted without affecting initiation-complex formation. The minimal sequences required for directing internal initiation in mammalian (rabbit reticulocyte lysate), plant (wheatgerm extract) and insect (Sf21 cells) translation systems have now been defined. A fragment (nt 426-579) from the 3' portion of the 5' UTR can direct translation in each of these translation systems. In addition, a distinct region (nt 300-429) is also active. Thus, unstructured regions within the 5' UTR seem to be critical for IRES function.
KW - 5' Untranslated Regions/genetics
KW - Animals
KW - Aphids/virology
KW - DNA, Viral/genetics
KW - Insect Viruses/genetics
KW - Mammals/virology
KW - Peptide Chain Termination, Translational
KW - Plants/virology
KW - Plasmids
KW - Protein Biosynthesis
KW - Viral Proteins/biosynthesis
U2 - 10.1099/vir.0.82682-0
DO - 10.1099/vir.0.82682-0
M3 - Journal article
C2 - 17412990
VL - 88
SP - 1583
EP - 1588
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - Pt 5
ER -
ID: 257918753