Hypoxia-induced metastasis model in embryonic zebrafish
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Hypoxia-induced metastasis model in embryonic zebrafish. / Rouhi, Pegah; Jensen, Lasse D.; Cao, Ziquan; Hosaka, Kayoko; Lanne, Toste; Wahlberg, Eric; Steffensen, John Fleng; Cao, Yihai.
In: Nature Protocols (Print), Vol. 5, No. 12, 01.12.2010, p. 1911-1918.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Hypoxia-induced metastasis model in embryonic zebrafish
AU - Rouhi, Pegah
AU - Jensen, Lasse D.
AU - Cao, Ziquan
AU - Hosaka, Kayoko
AU - Lanne, Toste
AU - Wahlberg, Eric
AU - Steffensen, John Fleng
AU - Cao, Yihai
PY - 2010/12/1
Y1 - 2010/12/1
N2 - Hypoxia facilitates tumor invasion and metastasis by promoting neovascularization and co-option of tumor cells in the peritumoral vasculature, leading to dissemination of tumor cells into the circulation. However, until recently, animal models and imaging technology did not enable monitoring of the early events of tumor cell invasion and dissemination in living animals. We recently developed a zebrafish metastasis model to dissect the detailed events of hypoxia-induced tumor cell invasion and metastasis in association with angiogenesis at the single-cell level. In this model, fluorescent DiI-labeled human or mouse tumor cells are implanted into the perivitelline cavity of 48-h-old zebrafish embryos, which are subsequently placed in hypoxic water for 3 d. Tumor cell invasion, metastasis and pathological angiogenesis are detected under fluorescent microscopy in the living fish. The average experimental time for this model is 7 d. Our protocol offers a remarkable opportunity to study molecular mechanisms of hypoxia-induced cancer metastasis.
AB - Hypoxia facilitates tumor invasion and metastasis by promoting neovascularization and co-option of tumor cells in the peritumoral vasculature, leading to dissemination of tumor cells into the circulation. However, until recently, animal models and imaging technology did not enable monitoring of the early events of tumor cell invasion and dissemination in living animals. We recently developed a zebrafish metastasis model to dissect the detailed events of hypoxia-induced tumor cell invasion and metastasis in association with angiogenesis at the single-cell level. In this model, fluorescent DiI-labeled human or mouse tumor cells are implanted into the perivitelline cavity of 48-h-old zebrafish embryos, which are subsequently placed in hypoxic water for 3 d. Tumor cell invasion, metastasis and pathological angiogenesis are detected under fluorescent microscopy in the living fish. The average experimental time for this model is 7 d. Our protocol offers a remarkable opportunity to study molecular mechanisms of hypoxia-induced cancer metastasis.
U2 - 10.1038/nprot.2010.150
DO - 10.1038/nprot.2010.150
M3 - Journal article
C2 - 21127485
VL - 5
SP - 1911
EP - 1918
JO - Nature Protocols
JF - Nature Protocols
SN - 1754-2189
IS - 12
ER -
ID: 34358922