Hypobromous acid and bromamine production by neutrophils and modulation by superoxide
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Hypobromous acid and bromamine production by neutrophils and modulation by superoxide. / Chapman, Anna L P; Skaff, Ojia; Senthilmohan, Revathy; Kettle, Anthony J; Davies, Michael Jonathan.
In: Biochemical Journal, Vol. 417, No. 3, 01.02.2009, p. 773-81.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Hypobromous acid and bromamine production by neutrophils and modulation by superoxide
AU - Chapman, Anna L P
AU - Skaff, Ojia
AU - Senthilmohan, Revathy
AU - Kettle, Anthony J
AU - Davies, Michael Jonathan
PY - 2009/2/1
Y1 - 2009/2/1
N2 - MPO (myeloperoxidase) catalyses the oxidation of chloride, bromide and thiocyanate to their respective hypohalous acids. We have investigated the generation of HOBr by human neutrophils in the presence of physiological concentrations of chloride and bromide. HOBr was trapped with taurine and detected by monitoring the bromination of 4-HPAA (4-hydroxyphenylacetic acid). With 100 microM bromide and 140 mM chloride, neutrophils generated HOBr and it accounted for approx. 13% of the hypohalous acids they produced. Addition of SOD (superoxide dismutase) doubled the amount of HOBr detected. Therefore we investigated the reaction of superoxide radicals with a range of bromamines and bromamides and found that superoxide radicals stimulated the decomposition of these species, with this occurring in a time- and dose-dependent manner. The protection afforded by SOD against such decay demonstrates that these processes are superoxide-radical-dependent. These data are consistent with neutrophils generating HOBr at sites of infection and inflammation. Both HOBr and bromamines/bromamides have the potential to react with superoxide radicals to form additional radicals that may contribute to inflammatory tissue damage.
AB - MPO (myeloperoxidase) catalyses the oxidation of chloride, bromide and thiocyanate to their respective hypohalous acids. We have investigated the generation of HOBr by human neutrophils in the presence of physiological concentrations of chloride and bromide. HOBr was trapped with taurine and detected by monitoring the bromination of 4-HPAA (4-hydroxyphenylacetic acid). With 100 microM bromide and 140 mM chloride, neutrophils generated HOBr and it accounted for approx. 13% of the hypohalous acids they produced. Addition of SOD (superoxide dismutase) doubled the amount of HOBr detected. Therefore we investigated the reaction of superoxide radicals with a range of bromamines and bromamides and found that superoxide radicals stimulated the decomposition of these species, with this occurring in a time- and dose-dependent manner. The protection afforded by SOD against such decay demonstrates that these processes are superoxide-radical-dependent. These data are consistent with neutrophils generating HOBr at sites of infection and inflammation. Both HOBr and bromamines/bromamides have the potential to react with superoxide radicals to form additional radicals that may contribute to inflammatory tissue damage.
KW - Bromates
KW - Bromides
KW - Humans
KW - Neutrophils
KW - Phenylacetates
KW - Spectrometry, Mass, Electrospray Ionization
KW - Superoxide Dismutase
KW - Superoxides
KW - Taurine
U2 - 10.1042/BJ20071563
DO - 10.1042/BJ20071563
M3 - Journal article
C2 - 18851713
VL - 417
SP - 773
EP - 781
JO - Biochemical Journal
JF - Biochemical Journal
SN - 0264-6021
IS - 3
ER -
ID: 129670548