Human recombinant antibodies against Plasmodium falciparum merozoite surface protein 3 cloned from peripheral blood leukocytes of individuals with immunity to malaria demonstrate antiparasitic properties
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Human recombinant antibodies against Plasmodium falciparum merozoite surface protein 3 cloned from peripheral blood leukocytes of individuals with immunity to malaria demonstrate antiparasitic properties. / Lundquist, Rasmus; Nielsen, Leif Kofoed; Jafarshad, Ali; Soesoe, Daw; Christensen, Lars Harder; Druilhe, Pierre; Dziegiel, Morten Hanefeld.
In: Infection and Immunity, Vol. 74, No. 6, 06.2006, p. 3222-31.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Human recombinant antibodies against Plasmodium falciparum merozoite surface protein 3 cloned from peripheral blood leukocytes of individuals with immunity to malaria demonstrate antiparasitic properties
AU - Lundquist, Rasmus
AU - Nielsen, Leif Kofoed
AU - Jafarshad, Ali
AU - Soesoe, Daw
AU - Christensen, Lars Harder
AU - Druilhe, Pierre
AU - Dziegiel, Morten Hanefeld
PY - 2006/6
Y1 - 2006/6
N2 - Immunoglobulins from individuals with immunity to malaria have a strong antiparasitic effect when transferred to Plasmodium falciparum malaria infected patients. One prominent target of antiparasitic antibodies is the merozoite surface antigen 3 (MSP-3). We have investigated the antibody response against MSP-3 residues 194 to 257 (MSP-3(194-257)) on the molecular level. mRNA from peripheral blood leukocytes from clinically immune individuals was used as a source of Fab (fragment antibody) genes. A Fab-phage display library was made, and three distinct antibodies designated RAM1, RAM2, and RAM3 were isolated by panning. Immunoglobulin G1 (IgG1) and IgG3 full-length antibodies have been produced in CHO cells. Reactivity with the native parasite protein was demonstrated by immunofluorescence microscopy, flow cytometry, and immunoblotting. Furthermore, the antiparasitic effect of RAM1 has been tested in vitro in an antibody-dependent cellular inhibition (ADCI) assay. Both the IgG1 and the IgG3 versions of the antibody show an inhibitory effect on parasite growth.
AB - Immunoglobulins from individuals with immunity to malaria have a strong antiparasitic effect when transferred to Plasmodium falciparum malaria infected patients. One prominent target of antiparasitic antibodies is the merozoite surface antigen 3 (MSP-3). We have investigated the antibody response against MSP-3 residues 194 to 257 (MSP-3(194-257)) on the molecular level. mRNA from peripheral blood leukocytes from clinically immune individuals was used as a source of Fab (fragment antibody) genes. A Fab-phage display library was made, and three distinct antibodies designated RAM1, RAM2, and RAM3 were isolated by panning. Immunoglobulin G1 (IgG1) and IgG3 full-length antibodies have been produced in CHO cells. Reactivity with the native parasite protein was demonstrated by immunofluorescence microscopy, flow cytometry, and immunoblotting. Furthermore, the antiparasitic effect of RAM1 has been tested in vitro in an antibody-dependent cellular inhibition (ADCI) assay. Both the IgG1 and the IgG3 versions of the antibody show an inhibitory effect on parasite growth.
KW - Adult
KW - Amino Acid Sequence
KW - Antibodies, Protozoan
KW - Antigens, Protozoan
KW - Blotting, Western
KW - Enzyme-Linked Immunosorbent Assay
KW - Epitope Mapping
KW - Flow Cytometry
KW - Humans
KW - Immunoglobulin Fab Fragments
KW - Immunoglobulin G
KW - Leukocytes
KW - Malaria
KW - Microscopy, Fluorescence
KW - Molecular Sequence Data
KW - Peptide Library
KW - Protozoan Proteins
KW - Recombinant Proteins
U2 - 10.1128/IAI.00928-05
DO - 10.1128/IAI.00928-05
M3 - Journal article
C2 - 16714549
VL - 74
SP - 3222
EP - 3231
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 6
ER -
ID: 47555907