Human embryonic stem cells have enhanced repair of multiple forms of DNA damage

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Human embryonic stem cells have enhanced repair of multiple forms of DNA damage. / Maynard, Scott; Swistowska, Anna Maria; Lee, Jae Wan; Liu, Ying; Liu, Su-Ting; Da Cruz, Alexandre Bettencourt; Rao, Mahendra; de Souza-Pinto, Nadja C; Zeng, Xianmin; Bohr, Vilhelm A.

In: Stem Cells, Vol. 26, No. 9, 01.09.2008, p. 2266-74.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Maynard, S, Swistowska, AM, Lee, JW, Liu, Y, Liu, S-T, Da Cruz, AB, Rao, M, de Souza-Pinto, NC, Zeng, X & Bohr, VA 2008, 'Human embryonic stem cells have enhanced repair of multiple forms of DNA damage', Stem Cells, vol. 26, no. 9, pp. 2266-74. https://doi.org/10.1634/stemcells.2007-1041

APA

Maynard, S., Swistowska, A. M., Lee, J. W., Liu, Y., Liu, S-T., Da Cruz, A. B., Rao, M., de Souza-Pinto, N. C., Zeng, X., & Bohr, V. A. (2008). Human embryonic stem cells have enhanced repair of multiple forms of DNA damage. Stem Cells, 26(9), 2266-74. https://doi.org/10.1634/stemcells.2007-1041

Vancouver

Maynard S, Swistowska AM, Lee JW, Liu Y, Liu S-T, Da Cruz AB et al. Human embryonic stem cells have enhanced repair of multiple forms of DNA damage. Stem Cells. 2008 Sep 1;26(9):2266-74. https://doi.org/10.1634/stemcells.2007-1041

Author

Maynard, Scott ; Swistowska, Anna Maria ; Lee, Jae Wan ; Liu, Ying ; Liu, Su-Ting ; Da Cruz, Alexandre Bettencourt ; Rao, Mahendra ; de Souza-Pinto, Nadja C ; Zeng, Xianmin ; Bohr, Vilhelm A. / Human embryonic stem cells have enhanced repair of multiple forms of DNA damage. In: Stem Cells. 2008 ; Vol. 26, No. 9. pp. 2266-74.

Bibtex

@article{891aea7adea74a26aeb01f4c6a1ca4ba,

title = "Human embryonic stem cells have enhanced repair of multiple forms of DNA damage",

abstract = "Embryonic stem cells need to maintain genomic integrity so that they can retain the ability to differentiate into multiple cell types without propagating DNA errors. Previous studies have suggested that mechanisms of genome surveillance, including DNA repair, are superior in mouse embryonic stem cells compared with various differentiated murine cells. Using single-cell gel electrophoresis (comet assay) we found that human embryonic stem cells (BG01, I6) have more efficient repair of different types of DNA damage (generated from H2O2, UV-C, ionizing radiation, or psoralen) than human primary fibroblasts (WI-38, hs27) and, with the exception of UV-C damage, HeLa cells. Microarray gene expression analysis showed that mRNA levels of several DNA repair genes are elevated in human embryonic stem cells compared with their differentiated forms (embryoid bodies). These data suggest that genomic maintenance pathways are enhanced in human embryonic stem cells, relative to differentiated human cells.",

keywords = "Cell Differentiation, Comet Assay, DNA Damage, DNA Repair, Embryonic Stem Cells, Fibroblasts, Ficusin, Hela Cells, Humans, Hydrogen Peroxide, Oligonucleotide Array Sequence Analysis, Radiation, Ionizing, Ultraviolet Rays",

author = "Scott Maynard and Swistowska, {Anna Maria} and Lee, {Jae Wan} and Ying Liu and Su-Ting Liu and {Da Cruz}, {Alexandre Bettencourt} and Mahendra Rao and {de Souza-Pinto}, {Nadja C} and Xianmin Zeng and Bohr, {Vilhelm A}",

year = "2008",

month = sep,

day = "1",

doi = "10.1634/stemcells.2007-1041",

language = "English",

volume = "26",

pages = "2266--74",

journal = "Stem Cells",

issn = "1066-5099",

publisher = "AlphaMed Press, Inc.",

number = "9",

}

RIS

TY - JOUR

T1 - Human embryonic stem cells have enhanced repair of multiple forms of DNA damage

AU - Maynard, Scott

AU - Swistowska, Anna Maria

AU - Lee, Jae Wan

AU - Liu, Ying

AU - Liu, Su-Ting

AU - Da Cruz, Alexandre Bettencourt

AU - Rao, Mahendra

AU - de Souza-Pinto, Nadja C

AU - Zeng, Xianmin

AU - Bohr, Vilhelm A

PY - 2008/9/1

Y1 - 2008/9/1

N2 - Embryonic stem cells need to maintain genomic integrity so that they can retain the ability to differentiate into multiple cell types without propagating DNA errors. Previous studies have suggested that mechanisms of genome surveillance, including DNA repair, are superior in mouse embryonic stem cells compared with various differentiated murine cells. Using single-cell gel electrophoresis (comet assay) we found that human embryonic stem cells (BG01, I6) have more efficient repair of different types of DNA damage (generated from H2O2, UV-C, ionizing radiation, or psoralen) than human primary fibroblasts (WI-38, hs27) and, with the exception of UV-C damage, HeLa cells. Microarray gene expression analysis showed that mRNA levels of several DNA repair genes are elevated in human embryonic stem cells compared with their differentiated forms (embryoid bodies). These data suggest that genomic maintenance pathways are enhanced in human embryonic stem cells, relative to differentiated human cells.

AB - Embryonic stem cells need to maintain genomic integrity so that they can retain the ability to differentiate into multiple cell types without propagating DNA errors. Previous studies have suggested that mechanisms of genome surveillance, including DNA repair, are superior in mouse embryonic stem cells compared with various differentiated murine cells. Using single-cell gel electrophoresis (comet assay) we found that human embryonic stem cells (BG01, I6) have more efficient repair of different types of DNA damage (generated from H2O2, UV-C, ionizing radiation, or psoralen) than human primary fibroblasts (WI-38, hs27) and, with the exception of UV-C damage, HeLa cells. Microarray gene expression analysis showed that mRNA levels of several DNA repair genes are elevated in human embryonic stem cells compared with their differentiated forms (embryoid bodies). These data suggest that genomic maintenance pathways are enhanced in human embryonic stem cells, relative to differentiated human cells.

KW - Cell Differentiation

KW - Comet Assay

KW - DNA Damage

KW - DNA Repair

KW - Embryonic Stem Cells

KW - Fibroblasts

KW - Ficusin

KW - Hela Cells

KW - Humans

KW - Hydrogen Peroxide

KW - Oligonucleotide Array Sequence Analysis

KW - Radiation, Ionizing

KW - Ultraviolet Rays

U2 - 10.1634/stemcells.2007-1041

DO - 10.1634/stemcells.2007-1041

M3 - Journal article

C2 - 18566332

VL - 26

SP - 2266

EP - 2274

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

IS - 9

ER -

ID: 33566563