Human anti-rhesus D IgG1 antibody produced in transgenic plants.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Human anti-rhesus D IgG1 antibody produced in transgenic plants. / Bouquin, Thomas; Thomsen, Mads; Nielsen, Leif Kofoed; Green, Trine Hefsgaard; Mundy, John; Dziegiel, Morten Hanefeld.

In: Transgenic Research, Vol. 11, No. 2, 2002, p. 115-22.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bouquin, T, Thomsen, M, Nielsen, LK, Green, TH, Mundy, J & Dziegiel, MH 2002, 'Human anti-rhesus D IgG1 antibody produced in transgenic plants.', Transgenic Research, vol. 11, no. 2, pp. 115-22. https://doi.org/10.1023/A:1015226418688

APA

Bouquin, T., Thomsen, M., Nielsen, L. K., Green, T. H., Mundy, J., & Dziegiel, M. H. (2002). Human anti-rhesus D IgG1 antibody produced in transgenic plants. Transgenic Research, 11(2), 115-22. https://doi.org/10.1023/A:1015226418688

Vancouver

Bouquin T, Thomsen M, Nielsen LK, Green TH, Mundy J, Dziegiel MH. Human anti-rhesus D IgG1 antibody produced in transgenic plants. Transgenic Research. 2002;11(2):115-22. https://doi.org/10.1023/A:1015226418688

Author

Bouquin, Thomas ; Thomsen, Mads ; Nielsen, Leif Kofoed ; Green, Trine Hefsgaard ; Mundy, John ; Dziegiel, Morten Hanefeld. / Human anti-rhesus D IgG1 antibody produced in transgenic plants. In: Transgenic Research. 2002 ; Vol. 11, No. 2. pp. 115-22.

Bibtex

@article{68276570954411dd86a6000ea68e967b,
title = "Human anti-rhesus D IgG1 antibody produced in transgenic plants.",
abstract = "Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.",
author = "Thomas Bouquin and Mads Thomsen and Nielsen, {Leif Kofoed} and Green, {Trine Hefsgaard} and John Mundy and Dziegiel, {Morten Hanefeld}",
note = "Keywords: Agglutination Tests; Arabidopsis; Blotting, Western; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Genetic Vectors; Humans; Immunoglobulin G; Monocytes; Plants, Genetically Modified; Recombinant Proteins; Respiratory Burst; Rh-Hr Blood-Group System",
year = "2002",
doi = "10.1023/A:1015226418688",
language = "English",
volume = "11",
pages = "115--22",
journal = "Transgenic Research",
issn = "0962-8819",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Human anti-rhesus D IgG1 antibody produced in transgenic plants.

AU - Bouquin, Thomas

AU - Thomsen, Mads

AU - Nielsen, Leif Kofoed

AU - Green, Trine Hefsgaard

AU - Mundy, John

AU - Dziegiel, Morten Hanefeld

N1 - Keywords: Agglutination Tests; Arabidopsis; Blotting, Western; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Genetic Vectors; Humans; Immunoglobulin G; Monocytes; Plants, Genetically Modified; Recombinant Proteins; Respiratory Burst; Rh-Hr Blood-Group System

PY - 2002

Y1 - 2002

N2 - Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.

AB - Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.

U2 - 10.1023/A:1015226418688

DO - 10.1023/A:1015226418688

M3 - Journal article

C2 - 12054345

VL - 11

SP - 115

EP - 122

JO - Transgenic Research

JF - Transgenic Research

SN - 0962-8819

IS - 2

ER -

ID: 6474713