Hippo signaling, actin polymerization, and follicle activation in fragmented human ovarian cortex
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Hippo signaling, actin polymerization, and follicle activation in fragmented human ovarian cortex. / Lunding, Stine A.; Andersen, Anders N.; Hardardottir, Lilja; Olesen, Hanna; Kristensen, Stine G.; Andersen, Claus Y.; Pors, Susanne E.
In: Molecular Reproduction and Development, Vol. 87, No. 6, 2020, p. 711-719.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Hippo signaling, actin polymerization, and follicle activation in fragmented human ovarian cortex
AU - Lunding, Stine A.
AU - Andersen, Anders N.
AU - Hardardottir, Lilja
AU - Olesen, Hanna
AU - Kristensen, Stine G.
AU - Andersen, Claus Y.
AU - Pors, Susanne E.
PY - 2020
Y1 - 2020
N2 - The Hippo pathway has been associated with regulation of early follicle growth. Studies of murine ovaries suggest that changes in the actin cytoskeleton, caused by fragmentation, result in inhibition of the Hippo pathway, and in turn, may activate follicle growth. In humans, the connections between fragmentation, the actin cytoskeleton, and follicle activation are yet to be confirmed. In this study, we investigated the impact in vitro fragmentation of a human ovarian cortex on (a) actin polymerization, (b) components of the Hippo pathway, and (c) follicle growth in vivo. The results showed that the ratio between globular and filamentous actin remained unchanged at all timepoints (0, 10, 30, 60, 120, and 240 min) following tissue fragmentation. Neither was the Hippo pathway effector protein YES-associated protein upregulated nor was gene expression of the downstream growth factors CCN2, CCN3, or CCN5 increased at any timepoint in the fragmented cortex. Furthermore, the number of growing follicles was similar in fragmented and intact cortex pieces after 6 weeks' xenotransplantation. However, the total number of surviving follicles was considerably lower in the fragmented cortex compared with intact tissue, suggesting detrimental effects of fragmentation on tissue grafting. These results indicate that fragmentation is likely to be ineffective to activate follicle growth in the human ovarian cortex.
AB - The Hippo pathway has been associated with regulation of early follicle growth. Studies of murine ovaries suggest that changes in the actin cytoskeleton, caused by fragmentation, result in inhibition of the Hippo pathway, and in turn, may activate follicle growth. In humans, the connections between fragmentation, the actin cytoskeleton, and follicle activation are yet to be confirmed. In this study, we investigated the impact in vitro fragmentation of a human ovarian cortex on (a) actin polymerization, (b) components of the Hippo pathway, and (c) follicle growth in vivo. The results showed that the ratio between globular and filamentous actin remained unchanged at all timepoints (0, 10, 30, 60, 120, and 240 min) following tissue fragmentation. Neither was the Hippo pathway effector protein YES-associated protein upregulated nor was gene expression of the downstream growth factors CCN2, CCN3, or CCN5 increased at any timepoint in the fragmented cortex. Furthermore, the number of growing follicles was similar in fragmented and intact cortex pieces after 6 weeks' xenotransplantation. However, the total number of surviving follicles was considerably lower in the fragmented cortex compared with intact tissue, suggesting detrimental effects of fragmentation on tissue grafting. These results indicate that fragmentation is likely to be ineffective to activate follicle growth in the human ovarian cortex.
KW - actin
KW - female infertility
KW - follicle activation
KW - Hippo pathway
KW - ovarian cortex
U2 - 10.1002/mrd.23353
DO - 10.1002/mrd.23353
M3 - Journal article
C2 - 32506789
AN - SCOPUS:85086044603
VL - 87
SP - 711
EP - 719
JO - Molecular Reproduction and Development
JF - Molecular Reproduction and Development
SN - 1040-452X
IS - 6
ER -
ID: 253404197